Gallic acid

Manufactured by Sisco Research Laboratories

Sourced in India

Gallic acid is a naturally occurring organic compound that is commonly used in the production of laboratory equipment. It is a trihydroxybenzoic acid with the chemical formula C6H2(OH)3COOH. Gallic acid is a white to light yellow crystalline solid that is soluble in water, alcohol, and ether.

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7 protocols using gallic acid

Quantifying Phenolic Compounds in S. pulcherrima

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The phenolic contents in the S. pulcherrima leaf extract were estimated using the Folin-Ciocalteu reagent. Two hundred microliters of the leaf extract at different concentrations (160 and 320 μg/ml prepared in distilled water) were mixed with 2% sodium carbonate (2 ml) and 10% Folin-Coicalteu reagent (2.5 ml). The reaction mixture was then incubated at 45°C in a water bath for 20 min. After the incubation, the absorbance was measured at 765 nm. All experiments were performed in triplicate, and gallic acid (Sisco Research Laboratories, India) was used as a standard.

Akhtar N., Mannan M.A., Pandey D., Sarkar A., Sharma H., Kumar M, & Ghosh A. (2023). Potent antifungal properties of gallic acid in Sarcochlamys pulcherrima against Candida auris. BioTechnologia, 104(2), 105-119.

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Physicochemical Analysis of Fresh Poniol Fruit

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Fresh Poniol (Flacourtia jangomas) fruit was obtained from the local market of Udalguri district, Assam, India, and stored at 4 °C for further use. Distilled water was used for washing of the fruit. All the chemicals and reagents were of analytical grade. The ethanol (99.9%) was supplied by Changshu Hongsheng Fine Chemicals Co., Ltd. (Changshu, China). Folin & Ciocalteu’s phenol reagent (2.0 N) and gallic acid (98%) were supplied by Sisco Research Laboratories Pvt. Ltd. (Taloja, Maharashtra, India). Sucrose (AR grade), sodium carbonate anhydrous (99.50–100.50%), and 2,2-Diphenyl-1-picrylhydrazyl or DPPH (85.0%) were supplied by Himedia Laboratories Pvt. Ltd. (LBS Marg, Mumbai, India). Ascorbic acid (99.0%) was purchased from Merck Life Science Pvt. Ltd. (Vikroli East, Mumbai, India). Deionized water was used to prepare all the chemical solutions.

Ali N.A., Dash K.K., Pandey V.K., Tripathi A., Mukarram S.A., Harsányi E, & Kovács B. (2023). Extraction and Encapsulation of Phytocompounds of Poniol Fruit via Co-Crystallization: Physicochemical Properties and Characterization. Molecules, 28(12), 4764.

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Antioxidant Evaluation of Natural Compounds

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2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′- azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS), 6-hydroxy-2,5,7,8-tetramethyl chroman-2-carboxylic acid (Trolox), Folin–Ciocalteu reagent, gallic acid, sodium carbonate, aluminum chloride, potassium acetate, quercetin, acetic acid, hexane, metaphosphoric acid, and 2,6-dichlorophenol indophenol sodium salt (all chemicals of AR grade) were procured from Sisco Research Laboratories Pvt. Ltd. (SRL), Mumbai, India. Throughout the experiment, deionized water that had been filtered through a 0.22 μm filter was used.

Saini R., Kaur S., Aggarwal P, & Dhiman A. (2023). The influence of conventional and novel blanching methods on potato granules, phytochemicals, and thermal properties of colored varieties. Frontiers in Nutrition, 10, 1178797.

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Enzymatic Assays for Oxidative Stress

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Gallic acid, 2,3,5-Triphenyl tetrazolium chloride (TTC), 5,5′-dithiobis-(2- nitrobenzoic acid) (DTNB), NADH, Phenazine methosulphate, were purchased from Sisco research laboratories Pvt. ltd. Sodium lauryl sulphate, Glacial acetic acid, dipotassium hydrogen phosphate, Tris buffer were purchased from Fisher scientific. Trichloro acetic acid, Sodium pyro phosphate, H₂O₂ were purchased from S D Fine-Chem Limited. N-butanol, Pottassium dihydrogen phosphate were purchased from Merck Life Science Pvt Ltd. All other chemicals were of the highest purity commercially available.

Praveen Kumar P., D. M., Siva Sankar Reddy L., Dastagiri Reddy Y., Somasekhar G., Sirisha N.V., Nagaraju K., Shouib M.S, & Rizwaan A.S. (2021). A new cerebral ischemic injury model in rats, preventive effect of gallic acid and in silico approaches. Saudi Journal of Biological Sciences, 28(9), 5204-5213.

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Antioxidant Capacity Determination Protocol

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Folin-Ciocalteu reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K₃Fe (CN)₆), trichloroacetic acid (TCA), ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.

Eshwarappa R.S., Iyer R.S., Subbaramaiah S.R., Richard S.A, & Dhananjaya B.L. (2014). Antioxidant activity of Syzygium cumini leaf gall extracts. BioImpacts : BI, 4(2), 101-107.

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Antioxidant Capacity Evaluation Protocol

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Folin-Ciocalteu (FC) reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, Gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K₃Fe (CN)₆), trichloroacetic acid (TCA) and ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.

Eshwarappa R.S., Iyer S., Subaramaihha S.R., Richard S.A, & Dhananjaya B.L. (2015). Antioxidant activities of ficus glomerata (moraceae) leaf gall extracts. Pharmacognosy Research, 7(1), 114-120.

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Antioxidant Activity Assay Protocol

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Folin-Ciocalteu reagent and quercetin were obtained from Qualigens, Mumbai, India. Ascorbic acid, Gallic acid, quercetin, L-Ascorbic acid, potassium thiocyanate, ethylene diamine tetra acetic acid (EDTA), 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, thiobarbituric acid (TBA), sodium nitroprusside, N-(1-Naphthyl) ethylenediamine dihydrochloride, potassium hexacyanoferrate (K 3 Fe (CN) 6 ), trichloroacetic acid (TCA), ferric chloride were procured from SRL Chemicals, India. All the other reagents and solvents were of analytical grade.

Eshwarappa R.S., Lakshmikantha R.Y., Subaramaihha S.R., Subbaiah S.G., Surendranath A.R, & Dhananjaya B.L. (2015). Antioxidant activity of insect gall extracts of Pistacia integerrima. Acta scientiarum polonorum. Technologia alimentaria, 14(4).

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