Lung tissues were fixed in 10% buffered formalin solution (pH7.2) for overnight, and serially sectioned to 15 μm at 20ºC. Immunohistochemistry was performed as described.33 (link) Immunoreactivity scores were analyzed using Image Scope V 10.0 software from Aperio Technologies (Vista, CA). The size and number of the metastases were also quantified and counted using Image Scope V 10.0 software (Aperio Technologies, Vista, CA).
Imagescope v 10
Manufactured by Leica
Sourced in United States
ImageScope V 10.0 is a digital pathology software solution designed for viewing and analyzing digital microscope slides. It provides basic functionality for navigating, annotating, and managing digital slide images.
Automatically generated - may contain errors
Lab products found in correlation
7 protocols using imagescope v 10
1
Quantitative Analysis of Lung Metastases
2
Melanoma Tissue Microarray Immunohistochemistry
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Human ME207, ME208 and ME1004b tissue microarrays (TMA) contain nevus, primary melanomas and lymph metastatic melanomas (US Biomax, Rockville, MD). TMA slides were stained with anti-PTEN, anti-Endpd5 anti-IGF1R and ATF6 antibodies and substrate DAB or AEC or permanent red (DAKO Cytomation, Carpinteria, CA). TMA slides were scanned by ImageScope and reviewed by three pathologists. Immunoreactivity scores were analyzed by three pathologists using ImageScope V 10.0 software (Aperio Technologies, Vista, CA). Immunostaining intensities were scored strong as 3; moderate as 2; week as 1 and negative as 0. Frequency of labeled tumor cells was scored 100% as 5, 66-99% as 4, 33-65% as 3, 10 -32% as 2, <10% as 1, no cell as 0. IHC scores equal intensity plus frequency.
3
Immunohistochemical Analysis of Tumor Tissues
4
Lung Tissue Immunohistochemistry and Metastasis Quantification
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Lung tissues were fixed in 10% buffered formalin solution (pH 7.2) for 16 h, and/or frozen in OCT compound and serially sectioned to 15 μm at 20°C. Immunohistochemistry was performed as described.69 (link) Immunoreactivity scores were analyzed using ImageScope V 10.0 software from Aperio Technologies (Vista, CA). The size of metastases was quantified by ImageJ software and analyzed using Statgraphics software.
5
Quantification of Myocardial Fibrosis
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After CMR imaging, all hearts were resected and fixed in 4% formaldehyde, and sliced in sections approximately 2-mm thick right at the midway between the apex and base of the LV on the short-axis plane. The sections were embedded in paraffin and were sliced further into 5-µm-thick sections. Afterwards, the specimens were stained with H&E and Masson’s trichrome staining. Histologic analysis was performed by a pathologist H.S.S. with 13 years of experience in thoracic pathology blinded to disease duration and CMR findings. H&E-stained sections were used for the overall examination. Masson’s trichrome-stained sections were digitally scanned with high-power magnification (400X) (ImageScope v10.0; Aperio Technologies, Vista, California, USA), and the pixel numbers of the myocardial tissue and collagen were counted using a macro written in ImageJ (National Institute of Health, Bethesda, Maryland, USA) based on the thresholding algorithms [22 (link), 23 (link)]. Collagen density for the RV was calculated as the percentage of pixel numbers of collagen to the myocardial tissue within a defined ROI, which was the same on T1 maps located at the middle one third level of the RV free wall.
6
Histological Analysis of Oviduct Pathology
7
Histological Analysis of Oviduct Pathology
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Oviduct pathology was analyzed 49 days post infection. Tissues were harvested, paraffin-embedded, and sectioned as described previously [19 (link)]. Sections were prepared and stained with either hematoxylin and eosin (H&E) or gomori trichrome. Slides were scanned at 20x and 40x magnification by the UCLA Translational Pathology Core Laboratory (TPCL) for analysis using ImageScope v 10.2 (Aperio Technologies). Sections stained with H&E were assessed for oviduct dilation, acute inflammation, and chronic inflammation. The diameter of each oviduct lumen was analyzed and measured by ImageScope, 6 mice per group were measured from H&E stained section collected transversally at the ovary to oviduct transition. Oviduct acute and chronic inflammation was evaluated semi-quantitatively with a score of 0 to 4, where 0 represents no inflammation and 4 represents high inflammation. Sections stained with gomori trichrome were assessed for oviduct fibrosis. The level of fibrosis was determined Sections stained with trichrome were evaluated using semi-quantitative scoring from 1 to 4 (1+ ≤ 25% light blue oviducts; 2+ ≥ 25% light blue oviducts; 3+ ≤ 25% dark blue oviducts; and 4+ ≥ 25% dark blue oviducts) [20 (link)–22 (link)].
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