previously reported [8 (link)] and seeded on collagen-coated 12-well plates (Becton Dickinson and Company) at a density of 2 × 105 cells in Williams’ Medium E (Thermo-Fisher Scientific, Waltham, MA) supplemented with 5% FBS and penicillin/streptomycin/amphotericin B solution (Gemini Bio-products). The cells were harvested and subjected to qRT-PCR for mRNA quantification.
Penicillin streptomycin amphotericin b solution
Manufactured by Gemini Bio
Penicillin/streptomycin/amphotericin B solution is a commonly used antibiotic and antimycotic solution for cell culture applications. It contains the antibiotics penicillin and streptomycin, as well as the antifungal agent amphotericin B. This solution is designed to prevent bacterial and fungal contamination in cell culture media.
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Lab products found in correlation
2 protocols using penicillin streptomycin amphotericin b solution
1
Quantifying mRNA Expression in Cells
2
Isolation and Culture of Primary Murine Hepatocytes
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