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Eia 3954

Manufactured by DRG International
Sourced in United States

The EIA-3954 is a laboratory equipment product. It is designed to perform Enzyme Immunoassay (EIA) tests. The core function of the EIA-3954 is to facilitate the detection and measurement of specific analytes in samples through immunological reactions.

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3 protocols using eia 3954

1

Cardiovascular Risk Factors and Lab Data

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We collected several demographics, cardiovascular risk factors, and laboratory data for all the participants. The demographics included age, gender, and body mass index (BMI). The cardiovascular risk factors included smoking, drinking, hypertension, diabetes mellitus (DM), prior myocardial infarction, and prior stroke, all of which came from the medical records of the participants. Diabetes mellitus was diagnosed according to the criteria by the American Diabetes Association (24 (link)). Triglycerides, cholesterol, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), albumin, creatinine, creatine kinase isoenzymes MB (CKMB), and pro-B-type natriuretic peptide (pro-BNP) were assayed using the Cobas Integra automated chemistry analyzer (Roche Cobas Integra 400 Plus, Roche Diagnostics, United States). High-sensitivity CRP was tested using an enzyme-linked immunosorbent assay according to the instructions of the manufacturer (EIA-3954, DRG International Inc., Springfield Township, United States). Blood samples were drawn from the patients within 24 h of admission. All of these laboratory tests were implemented using standard methods. Echocardiographs were performed on admission by experienced cardiologists.
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2

Cardiac Function and Inflammation Markers

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The QT interval was measured from the start of the Q wave to the end T wave in a normal beat. The RR is the time interval between two consecutive R waves, measured in seconds. Heart rate was calculated from average RR intervals of the beat within 10 seconds.
The QT interval and RR interval were measured by using Vernier Caliper, and the corrected QT interval (QTc) was calculated by using Bazettʼs formula [29] ; QTc (ms) = QT (ms) ⁄ √RR(s). The QTc is considered prolonged when it is longer than 440 ms [30, 31] .
Analysis of serum hs-CRP level was done by using the ELISA kit (EIA-3954, DRG International Inc., USA). Risk groups of CVDs have been defined by the cut-off points of hs-CRP level as <1.0 mg/l (low risk), and >1.0 mg/l (high risk).
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3

Biomarkers in Acute Coronary Syndrome

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Peripheral blood was sampled from patients within 24 h of admission prior to stent implantation. Triglycerides (TG), low-density lipoprotein cholesterol (LDL), high-sensitivity C-reactive protein (hsCRP), creatinine, Hemoglobin A1c (HbA1c), and pro-B-type natriuretic peptide (pro-BNP) were assayed using the Cobas Integra automated chemistry analyzer (Roche Cobas Integra 400 Plus, Roche Diagnostics, USA). High-sensitivity C-reactive protein was assessed using enzyme-linked immunosorbent assay according to the manufacturer's instructions (EIA-3954, DRG International Inc., Springfield Township, USA). Hemoglobin (HGB), MCV, and platelet counts were measured using the Sysmex KX 4000i haematology analyzer (Sysmex Corporation, Kobe, Japan) by standard biochemical techniques. The patients were further divided into 3 groups according to tertiles of MCV, and the reference MCV interval of our laboratory was 82-100 fL. Echocardiographs were performed on admission by experienced echo cardiologists, and systolic function was expressed as the ejection fraction (EF), which was calculated using Simpson's method.
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