c-MYC and centromere 8 copy number status of MCF-10A cell populations was determined by dual hybridisation with a Vysis LSI SpectrumOrange c-MYC Probe (Abbott Molecular, Maidenhead, UK; cat. no.: 05J545-011) and a CEP8 SpectrumGreen Probe (Abbott Molecular; cat. no.: 06J37-018) as recommended by the suppliers. c-MYC (orange), centromere 8 (aqua) and IGH (green) copy number status of HuMECs was determined using the Abbott IGH/MYC/CEP8 Tri-colour Dual Fusion Translocation Probe (Abbott Molecular). Slides were heated to 72 °C for 5 min and then incubated for 24 h at 37 °C in a humidified hybridisation chamber (HYBrite; Abbott Molecular). After hybridisation, slides were counterstained with 4′,6-diamidino-2-phenylindole (Vector Laboratories, Peterborough, UK). FISH was scored with an Olympus BX-61 fluorescence microscope (Olympus, Southend-on-Sea, UK) with a ×100 oil objective. Images were analysed using the CytoVision 7.2 SPOT counting system (Leica Microsystems, Gateshead, UK). A minimum of 100 (for MCF-10A) or 70 (for HuMECs) nuclei were scored per test by two independent analysts.
Igh myc cep8 tri colour dual fusion translocation probe
Manufactured by Abbott
The Abbott IGH/MYC/CEP8 Tri-colour Dual Fusion Translocation Probe is a laboratory tool designed to detect chromosomal abnormalities related to certain types of cancer. It utilizes fluorescence in situ hybridization (FISH) technology to visualize the presence and location of specific genetic sequences within cells.
Automatically generated - may contain errors
Lab products found in correlation
Hybrite, by Abbott (2 mentions)
Cytovision 7.2 spot counting system, by Leica (2 mentions)
Vysis lsi spectrumorange c myc probe, by Abbott (2 mentions)
Cep8 spectrumgreen probe, by Abbott (2 mentions)
4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (2 mentions)
Bx61 fluorescence microscope, by Olympus (2 mentions)
2 protocols using igh myc cep8 tri colour dual fusion translocation probe
1
Fluorescent In Situ Hybridization Assay for c-MYC and Centromere 8 in MCF-10A and HuMECs
2
Fluorescent in situ Hybridization Analysis of c-MYC and Centromere 8 in Breast Cell Lines
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c-MYC and centromere 8 copy number status of MCF-10A cell populations was determined by dual hybridisation with a Vysis LSI SpectrumOrange c-MYC Probe (Abbott Molecular, Maidenhead, UK; cat. no.: 05J545-011) and a CEP8 SpectrumGreen Probe (Abbott Molecular; cat. no.: 06J37-018) as recommended by the suppliers. c-MYC (orange), centromere 8 (aqua) and IGH (green) copy number status of HuMECs was determined using the Abbott IGH/MYC/CEP8 Tri-colour Dual Fusion Translocation Probe (Abbott Molecular). Slides were heated to 72 °C for 5 min and then incubated for 24 h at 37 °C in a humidified hybridisation chamber (HYBrite; Abbott Molecular). After hybridisation, slides were counterstained with 4′,6-diamidino-2-phenylindole (Vector Laboratories, Peterborough, UK). FISH was scored with an Olympus BX-61 fluorescence microscope (Olympus, Southend-on-Sea, UK) with a x100 oil objective. Images were analysed using the CytoVision 7.2 SPOT counting system (Leica Microsystems, Gateshead, UK). A minimum of 100 (for MCF-10A) or 70 (for HuMECs) nuclei were scored per test by two independent analysts.
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