Wang resin

Recombinant human HDAC8 from Sf9 cells was purchased from BPS Bioscience (cat#50008). HDAC inhibitors (PCI-34051, SAHA, Panobinostat, and Mocetinostat) were purchased from Selleckchem.com (cat#S2012, S1047, S1030, S1122, respectively). Solvents for peptide synthesis were purchased from Fisher unless otherwise indicated. Wang resin, Fmoc-protected amino acids, and derivatives were purchased from Chem-Impex.

Fmoc-protected amino acids for peptide synthesis were purchased from Advanced ChemTech (Louisville, KY), NovaBiochem (La Jolla, CA), or Aapptec (Louisville, KY). Rink amide resin (100–200 mesh, 0.3–0.6 meq/g), Wang resin (100–200 mesh, 1.0–1.4 meq/g), O-benzotriazole-N,N,N’,N’-tetramethyluronium hexafluorophosphate (HATU), and 1-hydroxybenzotriazole hydrate (HOBt) were purchased from Chem-Impex (Wood Dale, IL). Fluorescein succinimidyl ester (FAM), 5-(and-6)-carboxynaphthofluorescein succinimidyl ester (NF), and human serum were purchased from Sigma Aldrich (St. Louis, MO). Cell culture media, fetal bovine serum, penicillin-streptomycin, Dulbecco’s phosphate-buffered saline (DPBS) (2.67 mM potassium chloride, 1.47 mM potassium phosphate monobasic, 137 mM sodium chloride, 8.06 mM sodium phosphate dibasic), and 0.25% trypsin-EDTA were purchased from Invitrogen (Carlsbad, CA). Cell proliferation kit (MTT) was purchased from Roche (Indianapolis, IN). H358 and HCT116 were purchased from ATCC (Manassas, VA). CFBE410- was purchased from EMD Millipore (Burlington, MA). All solvents and other chemical reagents were obtained from Sigma-Aldrich, VWR (West Chester, PA), or Fisher Scientific and were used without further purification unless noted otherwise.