Doxycycline

The GC cell lines (MGC803, MKN45, HGC27, SGC7901, and AGS), normal gastric cells GES1, and HEK293T were obtained from the China Infrastructure of Cell Line Resources (Beijing, China). The GC cells were cultured in RPMI-1640 medium while the GES1 and HEK293T were cultured in DMEM medium. All the media were complemented with 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA), 100 units/mL penicillin, and 100 μg/mL streptomycin (Living, Beijing, China). All the cells were maintained in a humidified incubator containing 5% CO₂ at 37 °C. The cobalt chloride (#232696) and glycine (#V900144) were purchased from Sigma-Aldrich (St. Louis, MO, USA). The formic acid was purchased from Thermo Fisher Scientific (#85178, Waltham, MA, USA). The doxycycline was purchased from Topscience (#T1687, Shanghai, China).

A2780 and COV504 cells were purchased from the European Collection of Cell Cultures (Salisbury, UK). OVCAR8 cells were obtained from the National Cancer Institute (USA), KURAMOCHI cells were obtained from the Japanese Collection of Research Bioresources Cell Bank (Tokyo, Japan), and DOV13 cells were purchased from BioVector (BioVector NTCC). All other human cell lines were from the American Type Culture Collection. Short Tandem Repeat (STR) authentication of cell lines was done by the authors. Mouse ovarian carcinoma cell line ID8 was purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). The B16-OVA cell line (C57BL/6 mouse melanoma) was constructed by overexpression of OVA in B16 cells, and B3Z hybridoma cells were a gift from Nilabh Shastri (University of California, Berkeley, California, USA) (59 (link)). Cell lines were cultured in DMEM or RPMI 1640 medium supplemented with 10% FBS, 1% penicillin-streptomycin (Gibco), and maintained at 37°C in a humidified 5% CO₂ atmosphere and routinely tested negative for mycoplasma. Carboplatin were purchased from Selleck Chemicals. Doxycycline and verteporfin were from TopScience, respectively. Mouse IFN-β was obtained from R&D Systems. Stock solutions were diluted and stored according to the manufacturer’s protocols.