Polyclonal anti ha y 11

All the primary antibodies were used at a 1:1000 dilution, and secondary antibodies were diluted at 1:3000 dilution in 5% nonfat milk for immunoblotting assay. Anti-BAP1 (sc-28383), anti-N-Myc (sc-53993) and Polyclonal anti-HA (Y-11) were purchased from Santa Cruz Biotechnology. Anti-His (2365 S) antibodies were purchased from Cell Signaling. Monoclonal anti-HA antibody (MMS-101P) was purchased from Covance. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-Tubulin antibody (T-5168), anti-Vinculin antibody (V-4505), anti-Flag agarose beads (A-2220), anti-HA agarose beads (A-2095), peroxidase-conjugated anti-mouse secondary antibody (A-4416) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914) were purchased from Sigma.

Anti-BRAF (9433, 1:2000), anti-CRAF (9422, 1:2000), anti-pT202/pY204-ERK1/2 (4370, 1:3000), anti-ERK1/2 (4695, 1:3000), anti-pS217/pS221-MEK1/2 (9154, 1:3000), anti-MEK1/2 (9122, 1:3000), anti-SAPK/JNK (9252, 1:2000), anti-pT183/pY185-SAPK/JNK (4668, 1:2000), anti-c-Jun (9165, 1:2000), anti-JunB (3753, 1:2000), anti-ubiquitin (3933, 1:1000), anti-RAS (3339, 1:2000), anti-p-S259-CRAF (9421, 1:2000), anti-K48-linkage ubiquitin (D9D5, 1:2000), Human TNFα-neutralizing (D1B4) mAb (7321), and anti-GST (2622, 1:2000) antibodies were purchased from Cell Signaling Technology. Anti-FLIP (H-150, 1:2000), anti-pS63-c-Jun (KM-1, 1:2000), anti-p-S621-Raf-1 (E-1, 1:2000), anti-c-Myc (9E10, 1:2000), and polyclonal anti-HA (Y-11, 1:2000) antibodies were purchased from Santa Cruz. Anti-tubulin (T-5168, 1:2000) and anti-vinculin (V-4505, 1:2000) antibodies were purchased from Bethyl Labs. Polyclonal anti-Flag antibody (F-2425, 1:2000), monoclonal anti-Flag (F-3165, 1:2000) antibody, anti-Flag agarose beads (A-2220), and anti-HA agarose beads (A-2095), as well as peroxidase-conjugated anti-mouse secondary antibody (A-4416, 1:3000) and peroxidase-conjugated anti-rabbit secondary antibody (A-4914, 1:3000) were purchased from Sigma. Anti-K27-linkage ubiquitin (ab181537, 1:2000) was purchased from Abcam. Monoclonal anti-HA antibody (MMS-101P, 1:2000) was purchased from Covance.