Uv 550

The UV and IR spectra A were obtained using Jasco UV-550 and Perkin-Elmer model LE599 spectrometer, respectively. A Bruker DRX 500 or 700 MHz spectrometer were used for the analysis of NMR signals using methanol-d₄ as solvents. ESIMS data were measured on VG Autospec Ultima Mass spectrometers.
A RAW264.7 mouse macrophage cell line was obtained from Korean Cell Line Bank (No. 40071, Seoul, Korea), and a HaCaT human keratinocyte cell line was obtained from Cell Lines Service GmbH (No. 300493, Eppelheim, Germany). Griess reagent was purchased from Sigma Aldrich Co. (St. Louis, MO, USA). A TaqMan probe was obtained from Applied Biosystems (Foster City, CA, USA). To confirm the change in protein level, anti-IκB-α, anti-phospho-IκB-α, anti-ERK1/2, anti-phospho-ERK1/2, anti-JNK, anti-phospho-JNK and anti-GAPDH were obtained from Invitrogen (Carlsbad, CA, USA) and anti-p38 MAPK, anti-phospho-p38 MAPK, anti-STAT3 and anti-phospho-STAT3 were purchased from Cell Signaling (Danvers, MA, USA).

A JASCO DIP-1000 polarimeter was used for the measurement of optical rotations. A JASCO UV-550 and Perkin-Elmer model LE599 spectrometer were used respectively, for the measurement of UV and IR spectra. NMR spectra were recorded on a Bruker DRX 400, 500 or 700 MHz spectrometer using methanol-d₄ or acetone-d₆ as solvents. ESIMS data was obtained on VG Autospec Ultima mass spectrometers.