Dry cells of each biological replicate were transferred into digestion tubes (Gerhardt, UK), supplemented with 5ml of concentrated nitric acid (HNO3), and then incubated for at least 24h at room temperature with vortexing at 6h and 24h. Samples were placed on a water bath at 100 °C for 2h. After cooling, the final volume of each sample was adjusted to 10ml with distilled water and vortexed. Contents of sodium ions were measured by flame atomic absorption spectrometry (AAnalyst200, Perkin Elmer) in an air acetylene flame (Institute of Applied Geosciences, Aquatic Geochemistry, Karlsruhe Institute of Technology). Blank samples were prepared by adding 5ml of concentrated nitric acid to an empty digestion vessel and processed as described above.
Air acetylene flame
Manufactured by PerkinElmer
The air acetylene flame is a type of laboratory equipment used for atomic absorption spectroscopy. It is designed to provide a stable, high-temperature flame source that can be used to atomize and excite samples, allowing for the detection and quantification of various elements within the sample.
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Lab products found in correlation
2 protocols using air acetylene flame
1
Sodium Content Determination by AAS
2
Soil Fe Oxide Extraction and Analysis
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We measured soil Fe concentrations using a dithionite-citrate-bicarbonate (DCB) extraction to estimate "total extractable" Fe oxides (FeDCB) following the method by Darke and Walbridge (1994) (link). A separate extraction with acid ammonium oxalate (AAO) was used to estimate "poorly crystalline" Fe species (FeAAO; Coward et al. 2018; (link)Darke and Walbridge 1994; (link)Hall et al. 2018) (link). For both extractions, the supernatant was filtered through a 0.45 μm nylon filter (Tisch Scientific) and analyzed on an atomic absorption (AA) spectrometer on an air-acetylene flame (Perkin Elmer, Waltham, MA).
We also conducted inorganic Fe extractions on the mineral horizons to measure C associated with Fe concentrations that were unchanged across the transect; thus Al was not explored further (mean ± standard error: 2.8 ± 0.3 mg Al g -1 soil). As such, we use "organo-mineral associations" to refer to the relationship between C and Fe.
Extractions were conducted on a 0.5 g subsample of dried and ground soil. Extractants were filtered to 0.45 μm using a nylon filter (Tisch Scientific, North Bend, OH) and stored at 4 °C until analysis of organic C concentration with a TOC/TN Analyzer (Shimadzu Corporation, Kyoto, Japan; Sugimura and Suzuki 1988) . Fe concentrations were measured with AA spectrometry as above.
We also conducted inorganic Fe extractions on the mineral horizons to measure C associated with Fe concentrations that were unchanged across the transect; thus Al was not explored further (mean ± standard error: 2.8 ± 0.3 mg Al g -1 soil). As such, we use "organo-mineral associations" to refer to the relationship between C and Fe.
Extractions were conducted on a 0.5 g subsample of dried and ground soil. Extractants were filtered to 0.45 μm using a nylon filter (Tisch Scientific, North Bend, OH) and stored at 4 °C until analysis of organic C concentration with a TOC/TN Analyzer (Shimadzu Corporation, Kyoto, Japan; Sugimura and Suzuki 1988) . Fe concentrations were measured with AA spectrometry as above.
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