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Cb17 prkdcscid j mice

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The CB17-Prkdcscid/J mice are a strain of immunocompromised mice that lack functional B and T cells. They are commonly used as a model system for studying the immune system and evaluating the effects of treatments on immune responses.

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Lab products found in correlation

Cb17 prkdcscid j mice, by Jackson ImmunoResearch (2 mentions) Matrigel, by BD (2 mentions)

2 protocols using cb17 prkdcscid j mice

1

Xenograft of Kidney Organoids and Teratomas

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Kidney organoid cultures (400,000 cells) were dissociated with accutase, pelleted, resuspended in Advanced RPMI and injected into the kidneys of P0 NOD.CB17-Prkdcscid/J mice (Jackson Labs). The mice were sutured and killed 3 weeks later and the kidneys were sectioned. To form teratomas ∼2,000,000 undifferentiated hPSCs were dissociated, resuspended in 50 μl cold Matrigel (BD), and injected dorsally into 8-week-old NOD.CB17-Prkdcscid/J mice. Growths were collected 8–10 weeks later. Male and female animals were used. Experiments were performed in compliance with ethical regulations and ARRIVE guidelines and in accordance with protocols approved by the Harvard Medical Area Standing Committee on Animals.
Freedman B.S., Brooks C.R., Lam A.Q., Fu H., Morizane R., Agrawal V., Saad A.F., Li M.K., Hughes M.R., Werff R.V., Peters D.T., Lu J., Baccei A., Siedlecki A.M., Valerius M.T., Musunuru K., McNagny K.M., Steinman T.I., Zhou J., Lerou P.H, & Bonventre J.V. (2015). Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nature Communications, 6, 8715.
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2

Kidney Organoids and Teratoma Formation

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Kidney organoid cultures (400,000 cells) were dissociated with Accutase, pelleted, resuspended in Advanced RPMI, and injected into the kidneys of P0 NOD.CB17-Prkdcscid/J mice (Jackson Labs). The mice were sutured and sacrificed three weeks later and the kidneys were sectioned. To form teratomas, ∼ 2,000,000 undifferentiated hPSCs were dissociated, resuspended in 50 µl cold Matrigel (BD), and injected dorsally into 8-week old NOD.CB17-Prkdcscid/J mice. Growths were harvested 8–10 weeks later. Male and female animals were utilized. Experiments were performed in compliance with ethical regulations and ARRIVE guidelines and in accordance with protocols approved by the Harvard Medical Area Standing Committee on Animals.
Freedman B.S., Brooks C.R., Lam A.Q., Fu H., Morizane R., Agrawal V., Saad A.F., Li M.K., Hughes M.R., Werff R.V., Peters D.T., Lu J., Baccei A., Siedlecki A.M., Valerius M.T., Musunuru K., McNagny K.M., Steinman T.I., Zhou J., Lerou P.H, & Bonventre J.V. (2015). Modelling kidney disease with CRISPR-mutant kidney organoids derived from human pluripotent epiblast spheroids. Nature Communications, 6, 8715.
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