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Anti il 12 23 p40 neutralizing antibody

Manufactured by Thermo Fisher Scientific
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The Anti-IL-12/23 p40 neutralizing antibody is a laboratory reagent designed to neutralize the p40 subunit shared by interleukin-12 (IL-12) and interleukin-23 (IL-23). This antibody can be used in various in vitro research applications to study the biological functions of IL-12 and IL-23.

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Lab products found in correlation

Human tgf β1, by R&D Systems (3 mentions) Mouse il 2, by R&D Systems (3 mentions) Anti il 4 neutralizing antibody, by Thermo Fisher Scientific (3 mentions) Anti ifnγ neutralizing antibody, by Thermo Fisher Scientific (3 mentions) Soluble anti cd28, by Thermo Fisher Scientific (3 mentions) Anti cd3, by Thermo Fisher Scientific (3 mentions) Mouse il 6, by R&D Systems (3 mentions) Naive cd4 t cells isolation kit, by Miltenyi Biotec (2 mentions) Anti il 12 23 p40 neutralizing antibody, by R&D Systems (2 mentions) Mouse il 27, by R&D Systems (2 mentions) Recombinant dll4, by R&D Systems (2 mentions) Na ve cd4 t cells isolation kit, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Anti-IL-23 antibody Anti-IL-12 IL-23 IL-12

3 protocols using anti il 12 23 p40 neutralizing antibody

1

Differentiation of Naive T Cells into Th Subsets

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CD4+ CD25−CD62LhiCD44lo naive T cells were enriched from spleen using the naive CD4 T cells isolation kit (Miltenyi Biotec) with more than 92% purity. Naive T cells were then plated and cultured in 24-well plates. Naive T cells (106/0.5 mL) were stimulated with plate-bound anti-CD3 (2.5 μg/mL; eBioscience), soluble anti-CD28 (3 μg/mL; eBioscience), and plate-bound recombinant DLL4 (1.65 μg/mL, R&D). In addition, recombinant cytokines and neutralizing antibodies were added to skew toward different Th cells in vitro. For Th1: mouse IL-12 (10 ng/mL), anti-IL-4 neutralizing antibody (10 μg/mL; eBioscience); for Th2: mouse IL-4 (10 ng/mL; R&D System), anti-IFNγ neutralizing antibody (10 μg/mL; eBioscience), anti-IL-12/23 p40 neutralizing antibody (10 μg/mL); for Th17 cells: mouse IL-6 (10 ng/mL; R&D System), human TGF-β1 (2 ng/mL; R&D System), anti-IFNγ neutralizing antibody (10 μg/mL; eBioscience), anti-IL-4 neutralizing antibody (10 μg/mL; eBioscience), and anti-IL-12/23 p40 neutralizing antibody (10 μg/mL; eBioscience) were added; for IL-27-inducing TR1, mouse IL-27 (20 ng/mL, R&D) were added; to skew toward in vitro-iTreg cells (iTreg), human TGFβ1 (2 ng/mL; R&D System) and mouse IL-2 (10 ng/mL; R&D System) were added at the same time.
Ting H.A., de Almeida Nagata D., Rasky A.J., Malinczak C.A., Maillard I.P., Schaller M.A, & Lukacs N.W. (2018). Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection. Mucosal immunology, 11(5), 1524-1536.
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2

Differentiation of Naive T Cells into Th Subsets

Check if the same lab product or an alternative is used in the 5 most similar protocols
CD4+ CD25−CD62LhiCD44lo naive T cells were enriched from spleen using the naive CD4 T cells isolation kit (Miltenyi Biotec) with more than 92% purity. Naive T cells were then plated and cultured in 24-well plates. Naive T cells (106/0.5 mL) were stimulated with plate-bound anti-CD3 (2.5 μg/mL; eBioscience), soluble anti-CD28 (3 μg/mL; eBioscience), and plate-bound recombinant DLL4 (1.65 μg/mL, R&D). In addition, recombinant cytokines and neutralizing antibodies were added to skew toward different Th cells in vitro. For Th1: mouse IL-12 (10 ng/mL), anti-IL-4 neutralizing antibody (10 μg/mL; eBioscience); for Th2: mouse IL-4 (10 ng/mL; R&D System), anti-IFNγ neutralizing antibody (10 μg/mL; eBioscience), anti-IL-12/23 p40 neutralizing antibody (10 μg/mL); for Th17 cells: mouse IL-6 (10 ng/mL; R&D System), human TGF-β1 (2 ng/mL; R&D System), anti-IFNγ neutralizing antibody (10 μg/mL; eBioscience), anti-IL-4 neutralizing antibody (10 μg/mL; eBioscience), and anti-IL-12/23 p40 neutralizing antibody (10 μg/mL; eBioscience) were added; for IL-27-inducing TR1, mouse IL-27 (20 ng/mL, R&D) were added; to skew toward in vitro-iTreg cells (iTreg), human TGFβ1 (2 ng/mL; R&D System) and mouse IL-2 (10 ng/mL; R&D System) were added at the same time.
Ting H.A., de Almeida Nagata D., Rasky A.J., Malinczak C.A., Maillard I.P., Schaller M.A, & Lukacs N.W. (2018). Notch ligand Delta-like 4 induces epigenetic regulation of Treg cell differentiation and function in viral infection. Mucosal immunology, 11(5), 1524-1536.
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3

Isolation and Induction of Naïve T Cell Subsets

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CD4+CD25−CD62LhiCD44lo naïve T cells were enriched from spleen using the naïve CD4 T cells isolation kit (Miltenyi Biotec) with more than 92% purity. Naïve T cells were then plated and cultured in 24-well plates. 106 / 0.5 mL of naïve T cells were stimulated with plate-bound anti-CD3 (2.5 μg/mL; eBioscience), soluble anti-CD28 (3 μg/mL; eBioscience), and plate-bound recombinant Dll4 (1.65 μg/mL or the dose mentioned; R&D); to skew toward in vitro-induced Treg cells (iTreg), human TGFβ1 (2 ng/mL; R&D System) and mouse IL-2 (10 ng/mL; R&D System) were added at the same time; to re-stimulate toward in vitro-induced Th17 cells (Th17), mouse IL-6 (10 ng/mL; R&D System), human TGFβ1 (2 ng/mL; R&D System), anti-IFNγ neutralizing antibody (10 μg/mL; eBioscience), anti-IL-4 neutralizing antibody (10 μg/mL; eBioscience), and anti-IL-12/23 p40 neutralizing antibody (10 μg/mL; eBioscience) were added at 5 × 105/ 0.2 mL of viable iTreg culture or 105/ 0.2 mL sorted eGFP+ iTreg if mentioned.
Ting H.A., Schaller M.A., de Almeida Nagata D.E., Rasky A.J., Maillard I.P, & Lukacs N.W. (2017). Notch ligand Delta-like 4 promotes regulatory T cell identity in pulmonary viral infection. Journal of immunology (Baltimore, Md. : 1950), 198(4), 1492-1502.
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