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2 n morpholino ethanesulfonic acid mes monohydrate

Manufactured by Merck Group
Sourced in United States

2-(N-morpholino) ethanesulfonic acid (MES monohydrate) is a buffer compound commonly used in biological research and laboratory applications. It maintains a stable pH range and is often utilized in cell culture, protein purification, and other biochemical procedures.

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3 protocols using 2 n morpholino ethanesulfonic acid mes monohydrate

1

Targeted Delivery of Doxorubicin using PLGA-RGD Nanoparticles

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PLGA-COOH (molecular weight 12,000 Da) was obtained from Daigang Biomaterial Co. (Jinan, People's Republic of China). DOX was purchased from MACKLIN Biochemical Technology CO (Shanghai, People's Republic of China). RGD (RGDfk-FITC, c(RGDfk)) was purchased from GL Biochem Ltd (Shanghai, People's Republic of China). PFH, polyvinyl alcohol (PVA, molecular weight 26 kDa), 2-(N-morpholino) ethanesulfonic acid (MES monohydrate), N-(3-dimethyl-aminopropyl)-N’-ethylcarbodiimide hydrochloride (EDC), 1,1ʹ-dioctadecyl-3,3,3ʹ,3ʹ-tetramethylindo-tricarbocyanine iodide (DiR), 4ʹ,6-diamidino-2-phenylindole (DAPI), and N-hydroxysuccinimide (NHS) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). High glucose dulbecco’s modified eagle medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Co. (Carlsbad, CA, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Molecular Technology (Shanghai, People's Republic of China). Trichloromethane and isopropanol were purchased from Chongqing Chuandong Chemicals (Chongqing, People's Republic of China). All other reagents used in this work were of analytical grade and were used as received.
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2

Synthesis and Characterization of Carboxylic Acid Derivatives

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3-pyridinemethanol, potassium tert-butoxide, toluene, dichloromethane, ethylmalonic acid, 2,2-dimethylmalonic acid, methylsuccinic acid, glutaric acid, n-adipic acid, n-suberic acid, n-sebacic acid, n-octanoylglycine, n-decanoylglycine, glycine methylester hydrochloride, 2-ethylhexanoic acid, 2-ethyloctanoic acid, 4-ethyloctanoic acid, tetrahydrofurane, carbonyldiimidazole, 2-mercapto-ethanol, 2-(N-morpholino) ethanesulfonic acid (MES) monohydrate, and coenzyme A were from Sigma-Aldrich (now Merck). ethylmalonic acid (methyl-d3) was from Cambridge Isotope Laboratories. Methanol, acetonitrile, isopropanol (MS-grade), formic acid, and ammonium formate (all MS-grade) were from Biosolve. n-Hexane was from VWR chemicals. Ethylmalonyl-CoA was from CoALA Biosciences. Hydrochloric acid (37%) was from Merck. 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDAC) was from Thermo Fisher Scientific.
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3

PSMA-Targeted Paclitaxel-Loaded Nanomedicine

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PLGA–COOH (50: 50, MW = 12.3 kDa) was purchased from Jinan Daigang Biomaterial Co. (Shandong, China). Paclitaxel was purchased from Meilun Bio (Dalian, China). Perfluoropentane (PFP), polyvinyl alcohol (PVA), IR780, N-(3-dimethyl-aminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), 2-(N-morpholino) ethanesulfonic acid (MES monohydrate) and 1,1′-dioctadecyl-3,3,3′,3′ tetramethylindocarbocyanine perchlorate (DiI), and 4′,6-diamidino-2-phenylindole (DAPI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). PSMA monoclonal antibody was purchased from Cell Signaling Technology (USA). Goat anti-human IgG Fc (FITC) was purchased from Abcam (Shanghai, China). Cell Counting Kit-8 (CCK-8) was obtained from Dojindo Molecular Technology (Shanghai, China). RPMI-1640 mediumand fetal bovine serum (FBS) were purchased from Gibco (USA). All other reagents used in this work were of analytical grade and were used as received.
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