Sinigrin

Manufactured by Phytoplan

Sourced in Germany

Sinigrin is a chemical compound found in various plant species, such as mustard and broccoli. It is a glucosinolate, a type of secondary metabolite produced by plants. Sinigrin serves as a defense mechanism against herbivores and pathogens.

Automatically generated - may contain errors

Lab products found in correlation

Acetic acid, by Avantor (2 mentions) Allyl isothiocyanate, by Merck Group (2 mentions) Gluconasturtiin, by Phytoplan (2 mentions) Glucobrassicin, by Phytoplan (2 mentions) Bradford reagent, by Merck Group (1 mentions) Acetonitrile, by Avantor (1 mentions) Bovine serum albumine (bsa), by Merck Group (1 mentions) Hydrochloric acid (hcl), by Reanal (1 mentions) Phenethyl isothiocyanate, by Merck Group (1 mentions) Sodium hydroxide (naoh), by Reanal (1 mentions) Boric acid, by Reanal (1 mentions) Sodium deoxycholate, by Carl Roth (1 mentions) Ascorbic acid, by Avantor (1 mentions) Cysteamine, by Merck Group (1 mentions) Ammonium acetate, by Avantor (1 mentions) Gluconapin, by Phytoplan (1 mentions) Glucobrassicanapin, by Phytoplan (1 mentions) Progoitrin, by Phytoplan (1 mentions) Glucotropaeolin, by Phytoplan (1 mentions) Glucoerucin, by Phytoplan (1 mentions)

4 protocols using sinigrin

Quantification of Glucosinolates and Isothiocyanates

Check if the same lab product or an alternative is used in the 5 most similar protocols

For all applications, bidistilled water was used. The used chemicals listed below were all of at least analytical grade. Boric acid, monosodium phosphate monohydrate, ascorbic acid, sodium hydroxide and hydrochloric acid were obtained from Reanal (Budapest, Hungary). Acetic acid, Ncyclohexyl-ethanesulphonic acid (CHES), acetonitrile, mercaptoAcetic acid (thioglycolic acid) were bought from VWR Hungary (Budapest, Hungary). Sodium deoxycholate was from Carl-Roth (Karlsruhe, Germany). Sinigrin and gluconasturtiin were from Phytoplan (Heidelberg, Germany). Allyl isothiocyanate, phenethyl isothiocyanate, bovine serum albumine and Bradford reagent were from Sigma-Aldrich (St.Louis, MO, USA).

Gonda S., Kiss-Szikszai A., Szűcs Z., Nguyen N.M, & Vasas G. (2016). Myrosinase Compatible Simultaneous Determination of Glucosinolates and Allyl Isothiocyanate by Capillary Electrophoresis Micellar Electrokinetic Chromatography (CE-MEKC). Phytochemical analysis : PCA, 27(3-4).

+ Open protocol

+ Expand

Allyl Isothiocyanate Analytical Methods

Check if the same lab product or an alternative is used in the 5 most similar protocols

All reagents were of analytical purity. Allyl isothiocyanate and cysteamine were from Sigma-Aldrich (St. Louis, MO, USA). Congo red, bromocresol green and methyl red were from Reanal (Budapest, Hungary). Sinigrin was from Phytoplan (Heidelberg, Germany). Buffer components (disodium phosphate, ammonia, acetic acid, ammonium acetate) and ascorbic acid were from VWR (Debrecen, Hungary). As water, type I water (18.2 MΩ cm⁻¹) was used, which was produced by a Human Zeneer Power I water purification system (Human corporation, Seoul, Republic of Korea).

Gonda S., Szűcs Z., Plaszkó T., Cziáky Z., Kiss-Szikszai A., Vasas G, & M-Hamvas M. (2018). A Simple Method for On-Gel Detection of Myrosinase Activity. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(9), 2204.

+ Open protocol

+ Expand

Quantification of Glucosinolates in Plant Extracts

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Glucosinolates were analysed in the same extract as the EEP and NEPP. For the identification and quantification of aliphatic and indolic glucosinolates the HPLC methods described above for the analysis of (poly)phenols were followed [54 (link),55 ]. However, chromatograms were registered at 227 nm, using sinigrin and glucobrassicin (Phytoplan, Heidelberg, Germany) as standards for aliphatic and indolic glucosinolates, respectively. The content was expressed as mg/100 g d.w.

Núñez-Gómez V., González-Barrio R., Baenas N., Moreno D.A, & Periago M.J. (2022). Dietary-Fibre-Rich Fractions Isolated from Broccoli Stalks as a Potential Functional Ingredient with Phenolic Compounds and Glucosinolates. International Journal of Molecular Sciences, 23(21), 13309.

+ Open protocol

+ Expand

Analytical-Grade Chemical Extraction and Glucosinolate Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

All the chemicals used for extraction as well as analyses in this experiment were analytical-grade products from Fisher Scientific Korea Ltd. (Seoul, Republic of Korea) and Sigma-Aldrich (St. Louis, MO, USA). In addition, ten commercially available standard glucosinolates (sinigrin, progoitrin, gluconasturtiin, glucobarbarin, glucobrassicin, glucobrassicanapin, glucoberteroin, glucoerucin, gluconapin, and glucotro-paeolin) were obtained from Phytoplan Diehm & Neuberger GmbH (Heidelberg, Germany) and have a purity of ≥97%.

Kim S.H., Lee G.A., Subramanian P, & Hahn B.S. (2023). Quantification and Diversity Analyses of Major Glucosinolates in Conserved Chinese Cabbage (Brassica rapa L. ssp. pekinensis) Germplasms. Foods, 12(6), 1243.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!