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Sars cov 2 wuhan hu 1 envelope protein

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The SARS-CoV-2/Wuhan-Hu-1 envelope (E) protein is a structural protein of the SARS-CoV-2 virus. It plays a role in the assembly and release of viral particles.

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Lab products found in correlation

E coli bl21 de3 cells, by Thermo Fisher Scientific (3 mentions) Champion pet sumo plasmid, by Thermo Fisher Scientific (3 mentions) Sanger sequencing, by Genewiz (3 mentions)

3 protocols using sars cov 2 wuhan hu 1 envelope protein

1

Expressing SARS-CoV-2 Envelope Protein

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The gene encoding the full-length SARS-CoV-2/Wuhan-Hu-1 envelope (E) protein (NCBI reference sequence YP_009724392.1, residues 1–75) was purchased from Genewiz. The gene encoding the TM domain (residues 8–38, ETGTLIVNSVLLFLAFVVFLLVTLAILTALR) was isolated using PCR and cloned into a Champion pET-SUMO plasmid (Invitrogen). The plasmid was transfected into E. coli BL21 (DE3) cells (Invitrogen) to express the SUMO-ETM fusion protein containing an N-terminal His6 tag (Fig. S1a). The construct’s DNA sequence was verified by Sanger sequencing (Genewiz).
Mandala V.S., McKay M.J., Shcherbakov A.A., Dregni A.J., Kolocouris A, & Hong M. (2020). Structure and Drug Binding of the SARS-CoV-2 Envelope Protein in Phospholipid Bilayers. Research Square.
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2

SARS-CoV-2 Envelope Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
The gene encoding full-length SARS-CoV-2/Wuhan-Hu-1 envelope (E) protein (NCBI reference sequence YP_009724392.1, residues 1–75) was purchased from Genewiz. The gene encoding the TM domain (residues 8–38, ETGTLIVNSVLLFLAFVVFLLVTLAILTALR) was isolated using PCR and cloned into a Champion pET-SUMO plasmid (Invitrogen). The plasmid was transfected into E. coli BL21 (DE3) cells (Invitrogen) to express the SUMO-ETM fusion protein containing an N-terminal His6 tag (Extended Data Fig. 1a). The construct’s DNA sequence was verified by Sanger sequencing (Genewiz).
Mandala V.S., McKay M.J., Shcherbakov A.A., Dregni A.J., Kolocouris A, & Hong M. (2020). Structure and drug binding of the SARS-CoV-2 envelope protein transmembrane domain in lipid bilayers. Nature structural & molecular biology, 27(12), 1202-1208.
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3

SARS-CoV-2 Envelope Protein Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
The gene encoding full-length SARS-CoV-2/Wuhan-Hu-1 envelope (E) protein (NCBI reference sequence YP_009724392.1, residues 1–75) was purchased from Genewiz. The gene encoding the TM domain (residues 8–38, ETGTLIVNSVLLFLAFVVFLLVTLAILTALR) was isolated using PCR and cloned into a Champion pET-SUMO plasmid (Invitrogen). The plasmid was transfected into E. coli BL21 (DE3) cells (Invitrogen) to express the SUMO-ETM fusion protein containing an N-terminal His6 tag (Extended Data Fig. 1a). The construct’s DNA sequence was verified by Sanger sequencing (Genewiz).
Mandala V.S., McKay M.J., Shcherbakov A.A., Dregni A.J., Kolocouris A, & Hong M. (2020). Structure and drug binding of the SARS-CoV-2 envelope protein transmembrane domain in lipid bilayers. Nature structural & molecular biology, 27(12), 1202-1208.
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