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Ffa assay kit

Manufactured by Abcam
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Sourced in United States, United Kingdom

The FFA assay kit is a laboratory equipment product designed to measure free fatty acid (FFA) levels in biological samples. It provides a quantitative analysis of FFAs using a colorimetric detection method.

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Lab products found in correlation

Safire microplate reader, by Tecan (1 mentions) Extracellular oxygen consumption assay kit, by Abcam (1 mentions) Onetouch ultra2, by LifeScan (1 mentions)

Close spelling variants of this product

FFA quantification assay kit

5 protocols using ffa assay kit

1

Measuring Hepatocyte β-Oxidation Capacity

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Hepatocytes were isolated and cultured as described previously (Caldez et al., 2018 (link)). β-oxidation capabilities of isolated hepatocytes were then measured using the FFA Assay Kit (ab217602, Abcam) in combination with the Extracellular Oxygen Consumption Assay Kit (ab197243, Abcam) following manufacturer’s protocol. Signals were read using a TECAN Safire microplate reader with parameters as indicated in the assay protocol.
Ow J.R., Caldez M.J., Zafer G., Foo J.C., Li H.Y., Ghosh S., Wollmann H., Cazenave-Gassiot A., Ong C.B., Wenk M.R., Han W., Choi H, & Kaldis P. (2020). Remodeling of whole-body lipid metabolism and a diabetic-like phenotype caused by loss of CDK1 and hepatocyte division. eLife, 9, e63835.
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2

Atorvastatin and GGPP Metabolic Profiling

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Atorvastatin was purchased from Gödecke/Parke-Davis (Freiburg, Germany). GGPP was obtained from GlpBio (Montclair, NJ, USA). GGPP ELISA kit was from Shanghai Win-win Biotechnology (Shanghai, China). The FFA assay kit was purchased from Abcam (Cambridge, MA, USA).
Shu X., Wu J., Zhang T., Ma X., Du Z., Xu J., You J., Wang L., Chen N., Luo M, & Wu J. (2022). Statin-Induced Geranylgeranyl Pyrophosphate Depletion Promotes PCSK9–Dependent Adipose Insulin Resistance. Nutrients, 14(24), 5314.
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3

Intracellular FFA Quantification

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Changes in intracellular FFA levels were determined using an FFA Assay kit (Abcam). Briefly, cells were homogenized in a chloroform/Triton-X-100 solution and centrifuged (16,000× g, 5 min). The organic phase was collected, air dried (50 °C, 30 min), and vacuum dried (30 min) prior to dissolving in the fatty acid assay buffer solution and the addition of acetyl-coA synthase reagent. A 50 µL aliquot was removed, and absorbance was recorded on a microplate reader at (570 nm).
Attal N., Marrero E., Thompson K.J, & McKillop I.H. (2022). Role of AMPK-SREBP Signaling in Regulating Fatty Acid Binding-4 (FABP4) Expression following Ethanol Metabolism. Biology, 11(11), 1613.
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4

Metabolic Biomarkers Quantification

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Terminal serum samples were collected and blood glucose levels measured using OnetouchUltra2 (Lifescan, Milpitas, United States), and insulin levels assayed in serum using ELISA from Mercodia (Uppsala, Sweden). Serum concentration of free fatty acid (FFA) was measured using FFA assay kit (#ab65341) from Abcam (Cambridge, United Kingdom).
Fryklund C., Morén B., Shah S., Grossi M., Degerman E., Matthaeus C, & Stenkula K.G. (2021). EH Domain-Containing 2 Deficiency Restricts Adipose Tissue Expansion and Impairs Lipolysis in Primary Inguinal Adipocytes. Frontiers in Physiology, 12, 740666.
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5

Glucagon Secretion in Insulin-Induced Hypoglycemia

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Glucagon secretion was assessed in 10 weeks old male and female mice subjected to insulin-induced hypolgycemia (IIH). Mice were fasted for 6 h and blood was collected 1 h after i.p. saline or insulin (0.8 U/kg) injection into micro-centrifuge tubes containing aprotinin-A/EDTA. Inadequate i.p. injection or health problem were used as exclusion criteria. Plasma glucagon concentrations were assessed by ELISA (Mercodia, Uppsala, Sweden). Nicotinic acid (NA, 32 mg/kg, i.p.) injection was performed in 5 h-fasted males between 11 and 15 weeks of age. Free fatty acids (FFA) concentration in plasma was assessed using FFA Assay kit (abcam, #ab65341). Hyperinsulinemic-hypoglycemic clamps were performed in 15–16 weeks-old mice, with a 33% glucose solution being infused for 150 min through either the portal or femoral vein at an insulin injection rate of 18 mU/kg/min45 (link), after 5 h of fasting.
Strembitska A., Labouèbe G., Picard A., Berney X.P., Tarussio D., Jan M, & Thorens B. (2022). Lipid biosynthesis enzyme Agpat5 in AgRP-neurons is required for insulin-induced hypoglycemia sensing and glucagon secretion. Nature Communications, 13, 5761.
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