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Mab4343

Manufactured by Merck Group
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MAB4343 is a laboratory equipment product manufactured by Merck Group. It is designed for use in various scientific research and analysis applications. The core function of MAB4343 is to perform specific tasks related to the handling and processing of samples or materials in a laboratory setting. However, a detailed description of its intended use or capabilities cannot be provided in an unbiased and factual manner within the constraints of this request.

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Lab products found in correlation

Ab28028, by Abcam (1 mentions) Ab6142, by Abcam (1 mentions) Ab41621, by Abcam (1 mentions) Ab5804, by Merck Group (1 mentions) A11008, by Thermo Fisher Scientific (1 mentions) A11005, by Thermo Fisher Scientific (1 mentions) Ab143, by Merck Group (1 mentions) Ab3594, by Merck Group (1 mentions) Mab302, by Merck Group (1 mentions) Mab377, by Merck Group (1 mentions) Ab3623, by Merck Group (1 mentions) Mab345, by Merck Group (1 mentions) Mab 1621, by Merck Group (1 mentions) Ab9610, by Merck Group (1 mentions) Mab3418, by Merck Group (1 mentions) Mms 435p 250, by Fortrea (1 mentions) Ab8207, by Abcam (1 mentions) Ab200, by Abcam (1 mentions) Mab4360, by Merck Group (1 mentions) Mab4304, by Merck Group (1 mentions)

3 protocols using mab4343

1

Comprehensive Neuroanatomical Profiling of Cells

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Mouse anti-TUJ1 1:1000 (MMS-435P-250, Covance), rabbit anti-CHAT 1:1000 (AB 143, Millipore), mouse anti-NFM 1:500 (MAB1621, Millipore), rabbit anti-ISLET1 1:1000 (20670, Abcam), rabbit anti-GFAP 1:1000 (AB5804, Millipore), rabbit anti-AQP4 1:100 (AB3594, Millipore), rabbit anti-EAAT2 1:100 (ab41621, Abcam), mouse anti-GS 1:100 (MAB302, Millipore), mouse anti-O4 1:1000 (MAB345, Millipore), rabbit anti-IBA1 1:500 (019-19741, Wakochemical), rat anti-CD11b 1:200 (550282, BD pharmingen), mouse anti-MAP2 1:500 (MAB3418, Millipore), mouse anti-TDP-43 1:300 (gift from Dr J-P Julien’s laboratory), mouse anti-NeuN 1:500 (MAB377, Millipore), mouse anti-CNPase 1:200 (NE1020, Millipore), mouse anti-Nestin 1:200 (AB6142, Abcam), mouse anti-SOX2 1:500 (MAB4343, Millipore), mouse anti-Vimentin 1:1000 (AB28028, Abcam), rabbit anti-OLIG2 1:500 (AB9610, Millipore), rat anti-CD31 1:500 (550274, BD Biosciences), rabbit anti-cleaved caspase 3 1:200 AB3623, Millipore), Alexa fluor 488 goat anti-rabbit (A-11008, Life Technologies), Alexa fluor 594 goat anti-mouse (A-11005, Life Technologies)
Beaudet M.J., Yang Q., Cadau S., Blais M., Bellenfant S., Gros-Louis F, & Berthod F. (2015). High yield extraction of pure spinal motor neurons, astrocytes and microglia from single embryo and adult mouse spinal cord. Scientific Reports, 5, 16763.
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2

Pluripotency and Lineage Marker Analysis

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The procedure has been described in a previously published study30 (link). The primary antibodies used were as follows: anti-OCT4 (1:400, 2750,CST), anti-TRA-1-60 (1:300, MAB4360, EMD Millipore, Billerica, MA, USA), anti-SSEA-4 (1:100, MAB4304, Millipore), anti-SOX2 (1:1000, MAB4343, Millipore), anti-TRA-1-81 (1:200, 14-8883, eBioscience), anti-AFP (1:200, 14-6583, eBioscience), anti-α-SMA (1:100, ab8207, Abcam), anti-Tuj-1 (1:500, MAB1637, Millipore), anti-WRN(1:200, ab200, Abcam), anti-γH2AX (1:200, 9718, CST), anti-RPA32(1:400, 2208, CST), anti-Rad 51 (1:400, sc-8349, Santa Cruz), anti-NANOG (1:400, ab80892, Abcam), and anti-FOXA2 (1:400, 3143, CST).
Wang S., Liu Z., Ye Y., Li B., Liu T., Zhang W., Liu G.H., Zhang Y.A., Qu J., Xu D, & Chen Z. (2018). Ectopic hTERT expression facilitates reprograming of fibroblasts derived from patients with Werner syndrome as a WS cellular model. Cell Death & Disease, 9(9), 923.
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3

Comprehensive Protein Expression Analysis

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Western blotting was performed with specific antibodies against human STAT3 (9132, Cell Signaling), phosphorylated STAT3 (9138, Cell Signaling), Oct3/4 (sc-5279, Santa Cruz biotechnology), Nanog (ab21624, Abcam), Sox2 (MAB4343, Millipore), H3K79me2 (ab3594, Abcam), H3K9me2 (ab1220, Abcam), H3K9me3 (ab8898, Abcam), H4K20me3 (07-463, Millipore), H3K27me3 (07-449, Millipore), H3K36me3 (ab9050, Abcam), acetyl-Histone H3 (06-599, Millipore), Histone H3 (9715, Cell Signaling), β-Actin (A5441, Sigma), Dot1L (ab72454, Abcam), and Cleaved Notch1 (NICD, ab52301, Abcam). Signals were detected by ECL reagents (GE Healthcare, Buckinghamshire, UK).
Kryczek I., Lin Y., Nagarsheth N., Peng D., Zhao L., Zhao E., Vatan L., Szeliga W., Dou Y., Owens S., Zgodzinski W., Majewski M., Wallner G., Fang J., Huang E, & Zou W. (2014). IL-22+CD4+ T cells promote colorectal cancer stemness via STAT3 transcription factor activation and induction of the methyltransferase DOT1L. Immunity, 40(5), 772-784.
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