PDLSCs were starved overnight and then cultured in the medium with 4 μg/ml Dil-acLDL (Yeasen Biotechnology, Shanghai, 20606ES76). After 4h culture, cells were fixed and analyzed under a ZEISS fluorescence microscope.
Dil acldl
Manufactured by Yeasen
Sourced in China
Dil-acLDL is a laboratory reagent used to detect and measure low-density lipoprotein (LDL) levels. It is a modified form of acetylated LDL (acLDL) that is designed for use in cellular assays and other analytical techniques.
Automatically generated - may contain errors
3 protocols using dil acldl
1
Dil-acLDL Uptake in PDLSCs
2
Dil-Ac-LDL Uptake Assay
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Cells were incubated with 20 μg/mL Dil-Ac-LDL (human Dil-Acetylated Low Density Lipoprotein, YEASEN, Shanghai, China) in EGM-2 for 4 h at 37 °C. Thereafter, cells were washed three times with PBS and visualized with a Nikon 80i fluorescence microscope.
3
Endothelial Cell Characterization Assay
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EPCs were washed with phosphate-buffered saline (PBS) three times, and incubated in medium containing 20 μg/ml Dil-ac-LDL (YEASEN Biotech, China) for 4 h at 37 °C, 5% CO2. Cells were fixed with 4% paraformaldehyde and incubated for 1 h with 10 μg/ml FITC-UEA-1 (Invitrogen, USA) at room temperature. Nuclei were labeled with DAPI (C0065, Solarbio, China). The incorporation of Dil-ac-LDL and binding of FITC-UEA-1 were assessed and photos were taken in an Intelligent Full-Automatic Fluorescence Microscopy Imaging System (Invitrogen™ EVOS™ FL Auto 2, Thermo Scientific, USA).
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