Mgso4 7h2o

Analytical grade Na₂SeO₃ (≥99.0%), HCl (≥37%), Glucose anhydrous (≥99.0%), Sucrose anhydrous (≥99.0%), NaCl (≥99.0%), KCl, MgSO₄×7H₂O (≥98.0%), TrisHCl (≥99.0%), NaOH (≥98.0%), Na₂S(≥99.0%), Na₂HPO₄×12H₂O (≥99.0%) and KH₂PO₄ (≥99.5%), were purchased from Sinopharm Chemical Reagent Co. Ltd, China. Biological grade peptone was acquired from Beijing Aoboxing Biotechnology Co. Ltd. Biological grade agar was produced by the Beijing Kangbeisi Technology Co. Ltd. Yeast Extract LP0021 was from Oxoid, made in France. Potassium persulfate (≥99.0%) and 2,2-Diphenyle-1-picrylhydrazyl (DPPH, ≥95.0%) were purchased from Sigma-Aldrich.

All samples were collected between September and October 2021; the samples were transported at room temperature to the lab and stored at 4 °C before use. Three samples were taken from Linfen (Shanxi, China), including expJ from a chicken stable, expY from a sheep stable and expT from a wheat field. The sample expM was taken from a horse stable in Hangzhou (Zhejiang, China). ExpJ, expY and expM were materials of stable soil with animal waste and ExpT was the soil in the wheat field; the samples were collected with a grid soil-sampling method composed of four parallel transects [40 (link)] in closed bags with the aseptic technique.
Deoxynivalenol (≥99.9%), (Triple Chemical Corp. Ltd., Guelph, ON, Canada), Acetonitrile (>99.9%), (Anhui Tiandi High Purity Organic Solvent Co., Ltd., Anqing, China), high-performance liquid chromatography (HPLC), and a Waters e2695 and Waters 2998 photodiode array detector (Waters Shanghai Corp., Ltd., Shanghai, China) were used.
Culture medium: NaHCO₃ 0.06 g/L (Sinopharm Group Chemical Reagent Co., Ltd., Shanghai, China), KCl 0.01 g/L (Shanghai Lingfeng Chemical Reagent Co., Ltd., Shanghai, China), MgSO₄·7H₂O 0.12 g/L (Sinopharm Group Chemical Reagent Co., Ltd.), and CaCl₂ 0.29 g/L (Chengdu Kelon Chemical Co., Ltd., Chengdu, China); pH 7.8 ± 0.2.

For the cathode electrolyte, 0.5 g of potassium ferricyanide (Macklin biochemical Co., Ltd, China, Shanghai) was dissolved in 100 ml of phosphate buffer solution (PBS) with a pH of 6.5 to obtain a 5 g/L solution. The anode electrolyte contained basal medium (7.5 g/L NH₄SO₄ (Macklin biochemical Co., Ltd, China, Shanghai), 14.4 g/L KH₂PO₄ (Aladdin Biochemical Technology Co., Ltd, China, Shanghai), 0.5 g/L MgSO₄·7H₂O (Sinopharm Chemical Reagent Co., Ltd, China, Beijing), 0.1% vitamin solution, and 0.1% trace metal solution [62 (link)]). The anode electrolyte was adjusted to a pH of 6.5 with KOH and autoclaved at 116 °C for 25 min.

The reference standard of ECH was purchased from Shanghai YuanYe Biotechnology Co., Ltd. (Shanghai, China); glucose and peptone were purchased from Beijing AoBoXing Bio-Tech Co. Ltd. (analytical grade, Beijing, China); KH₂PO₄ and MgSO₄·7H₂O were purchased from Sinopharm Chemical Reagent Co. Ltd. (analytical grade, Shanghai, China); methanol, ethanol, acetonitrile, and formic acid were purchased from Sinopharm Chemical Reagent Co. Ltd. (chromatographic grade, Shanghai, China); and HgCl₂ was purchased from Tianjin dongfang chemical factory (analytical grade, Tianjin, China).
TDL-5-A centrifuge (ShangHai Anting Scientific Instrument Factory, Shanghai, China); L-2000 Elite high performance liquid chromatograph (Hitachi Limited, Tokyo, Japan); KQ 5200E ultrasound cleaner (Kunshan ultrasonic instrument Co. Ltd., Kunshan, China); LRH-250-Z incubator (medical equipment factory in Guangdong, China); and HX-I incubator shaker (medical equipment factory in Guangdong, China) comprise the used equipment.

Zymomonas mobilis ZM4 (ATCC 31821) was revived from frozen glycerol stocks in 10 mL RM^OXOID (50 g/L glucose, 10 g/L YE, 2 g/L KH₂PO₄) at 30°C for about 6∼8 h prior to inoculating overnight seed cultures at 30°C at 100 rpm in RM^OXOID using shake flasks with a sealing gas permeable membrane sealed filled to 80% capacity. Glucose, KH₂PO₄, K₂HPO₄, NaCl, MgSO₄⋅7H₂O, Na₂MoO₄⋅2H₂O, and NH₄Cl were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). YE was purchased from OXOID, Becton Dickinson, and Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China). Tryptone was purchased from OXOID. Peptone was purchased from Becton Dickinson. Corn steep liquid (CSL) was purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China).
The recipes of different rich medium (RM) and minimal medium (MM) with different nitrogen sources used in this work were listed in Tables 5, 6, respectively.

The 12 isolates were incubated on fresh PDA cultures for 3 days at 30 °C. Then, the spore suspensions were washed using sterile normal saline and adjusted to a cell density of 10⁵~10⁶ by counting board under a microscope and 5 μL were taken for inoculation in a YES toxigenic medium (yeast extract 20 g, sucrose 150 g, MgSO₄·7H₂O 0.5 g(Sinopharm Inc., Shanghai, China), ZnSO₄·7H₂O 0.01 g (Sinopharm Inc., Shanghai, China), CuSO₄·5H₂O 0.005 g (Sinopharm Inc., Shanghai, China), agar 20 g (Sinopharm Inc., Shanghai, China) and l L distilled water) and a CYA toxigenic medium (NaNO₃ 3 g, K₂HPO₄ 1 g (Sinopharm Inc., Shanghai, China), KCl 0.5 g (Sinopharm Inc., Shanghai, China), MgSO₄·7H₂O 0.5 g, FeSO₄·7H₂O 0.01 g (Sinopharm Inc., Shanghai, China), ZnSO₄·7H₂O 0.01 g, CuSO₄·5H₂O 0.005 g, yeast extract (difco) 5 g, sucrose 30 g, agar 20 g and l L distilled water) and cultured at 30 °C for 7 days. The mycelium products were extracted and quantitatively detected by UPLC-MS/MS.