Il13rα2

After fixation in 10% formalin for 48 hours, the tissue samples were processed with histological embedding techniques and cut into 4 µm-thick sections. The slides were deparaffinized in xylene and rehydrated in graded alcohols. For antigen retrieval, the sections were autoclaved using a high-pressure method for 15 minutes in a commercially available 0.01 M sodium citrate solution (pH 6.0). Subsequently, the slides were incubated with 3% hydrogen peroxide in phosphate-buffered saline (PBS, pH 7.4) for 10 minutes to quench endogenous peroxidases. Slides were then probed with the primary antibody against IL13Rα2 (Abcam, Cambridge, UK) or VEGF (Abcam) for 1 hour at room temperature, followed by horseradish peroxidase-labeled secondary antibody for another 30 minutes at room temperature. After the antibody–antigen reaction, chromogen diaminobenzidine was applied to the slides. Finally, the sections were counterstained with 10% Harris hematoxylin, dehydrated, and mounted.