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αil 4 clone 11b11

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αIL-4 (clone 11B11) is a monoclonal antibody that binds to the cytokine interleukin-4 (IL-4). It can be used for research applications that involve the detection or neutralization of IL-4.

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Lab products found in correlation

Rmil 12, by Thermo Fisher Scientific (2 mentions) Rmil 6, by Thermo Fisher Scientific (2 mentions) αifn γ clone xmg1, by BioXCell (2 mentions) αcd3 clone 17a2, by BioXCell (2 mentions) αcd28 clone 37, by BioXCell (2 mentions) αtgf β clone 1d11, by BioXCell (2 mentions) αil 12 clone r1 5d9, by BioXCell (2 mentions) Cd4 t cell isolation kit, by Miltenyi Biotec (2 mentions) Multiskan fc multiplate photometer, by Thermo Fisher Scientific (1 mentions) Elisa development kit, by Thermo Fisher Scientific (1 mentions)

3 protocols using αil 4 clone 11b11

1

Cytokine Quantification by ELISA

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IL-13 concentrations were measured with an ELISA Development Kit (Peprotech). IL-4 ELISA were performed with α -IL-4 (clone 11B11, BioXcell) as coating antibody and biotinylated α-IL-4 (clone BVD6-24G2, Southern Biotech, Birmingham, AL) as detection Ab. Alkaline phosphatase-coupled streptavidin and para-Nitrophenylphosphat substrate (both from Southern Biotec) were used for detection. GM-CSF concentrations were measured with a commercial ELISA kit (R&D Systems). ELISAs were measured with a Multiskan FC multiplate photometer (ThermoScientific, Waltham, MA).
Willebrand R, & Voehringer D. (2016). IL-33-Induced Cytokine Secretion and Survival of Mouse Eosinophils Is Promoted by Autocrine GM-CSF. PLoS ONE, 11(9), e0163751.
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2

Naïve SMARTA Cell Polarization Protocols

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Naïve SMARTA cells were negatively isolated by using CD4+ T cell isolation kit (Miltenyi or StemCell). 2 × 106 SMARTA cells were seeded on 24 well plates coated with 8 µg/mL αCD3 (clone 17A2, BioXcell) and αCD28 (clone 37.51, BioXcell). For TH1polarization, Smarta cells were given with 20 µg/mL of αIL-4 (clone 11B11, BioXcell) and αTGF-β (clone 1D11, BioXcell) and 20 ng/mL of rmIL-12 (Peprotech). For IL-6 condition, 10 µg/mL of αIFN-γ (clone XMG1.2, BioXcell) and αIL-12 (clone R1-5D9, BioXcell) and 20 ng/mL of rmIL-6 (Peprotech) were added in culture media.
Choi Y.S., Gullicksrud J.A., Xing S., Zeng Z., Shan Q., Li F., Love P.E., Peng W., Xue H.H, & Crotty S. (2015). LEF-1 and TCF-1 orchestrate T follicular helper cell differentiation by regulating differentiation circuits upstream of Bcl6. Nature immunology, 16(9), 980-990.
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3

Naïve SMARTA Cell Polarization Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols
Naïve SMARTA cells were negatively isolated by using CD4+ T cell isolation kit (Miltenyi or StemCell). 2 × 106 SMARTA cells were seeded on 24 well plates coated with 8 µg/mL αCD3 (clone 17A2, BioXcell) and αCD28 (clone 37.51, BioXcell). For TH1polarization, Smarta cells were given with 20 µg/mL of αIL-4 (clone 11B11, BioXcell) and αTGF-β (clone 1D11, BioXcell) and 20 ng/mL of rmIL-12 (Peprotech). For IL-6 condition, 10 µg/mL of αIFN-γ (clone XMG1.2, BioXcell) and αIL-12 (clone R1-5D9, BioXcell) and 20 ng/mL of rmIL-6 (Peprotech) were added in culture media.
Choi Y.S., Gullicksrud J.A., Xing S., Zeng Z., Shan Q., Li F., Love P.E., Peng W., Xue H.H, & Crotty S. (2015). LEF-1 and TCF-1 orchestrate T follicular helper cell differentiation by regulating differentiation circuits upstream of Bcl6. Nature immunology, 16(9), 980-990.
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