Zetasizer nano zs machine

Manufactured by Malvern Panalytical

Sourced in United Kingdom

The Zetasizer Nano ZS is a laboratory instrument designed for the measurement of particle size, zeta potential, and molecular weight. It utilizes the principles of dynamic light scattering and electrophoretic light scattering to determine these properties. The instrument can analyze a wide range of samples, including suspensions, emulsions, and macromolecular solutions.

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Close spelling variants of this product

Zetasizer Nano ZS (5740 citations) Nano ZS (1243 citations) Zetasizer (953 citations) Zetasizer Nano (836 citations) Nano ZS Zetasizer (223 citations) Zetasizer ZS (91 citations) Nano ZS machine (2 citations) ZetaSizer machine (2 citations)

18 protocols using zetasizer nano zs machine

Nanoparticle Characterization by DLS

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Nanoparticle
sizes and
distributions were analyzed using dynamic light scattering (DLS) with
a Malvern Zetasizer Nano-ZS machine equipped with a red laser (633
nm) detecting backscattering at an angle of 173°. All measurements
were performed at 25 °C with an equilibration time of 60 s before
the experiment. Data are the average from 7 measurements, consisting
of at least 10 subruns of 10 s each. The analysis model applied was
general purpose (normal resolution) with the assumption of spherical
particles. All samples were diluted in buffer consisting of 50 mM
Tris-HCl, pH 8, 150 mM NaCl (viscosity 0.96066 cP, RI 1.332), and
the material was approximated as polystyrene latex (RI 1.59, abs 0.01).
Nanodisc samples contained ∼2 mM phospholipid (DMPC) and ∼0.4%
(w/v) polymer and were allowed to reach equilibrium by incubation
at 30 °C for 1 h with shaking at 650 rpm, followed by incubation
in the fridge (4 °C) for at least 1 day. Polymer only samples
had a concentration of ∼0.4% (w/v) polymer and were allowed
to equilibrate by the same treatment as performed on the nanodiscs.

Kopf A.H., Lijding O., Elenbaas B.O., Koorengevel M.C., Dobruchowska J.M., van Walree C.A, & Killian J.A. (2022). Synthesis and Evaluation of a Library of Alternating Amphipathic Copolymers to Solubilize and Study Membrane Proteins. Biomacromolecules, 23(3), 743-759.

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Characterization of Biosynthesized Gold Nanoparticles

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The size distribution through DLS (Dynamic Light Scattering) and the zeta potential of the biosynthesized SSP-AuNPs were evaluated by the zeta potential machine (Malvern Zetasizer Nano-ZS machine, Malvern, UK) at the temperature of 25°C.

Das G., Seo S., Yang I.J., Nguyen L.T., Shin H.S, & Patra J.K. (2023). Synthesis of Biogenic Gold Nanoparticles by Using Sericin Protein from Bombyx mori Silk Cocoon and Investigation of Its Wound Healing, Antioxidant, and Antibacterial Potentials. International Journal of Nanomedicine, 18, 17-34.

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Characterization of Carbon Nanohorns

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Unless otherwise noted, all of the reactions were conducted in a dry reaction flask made of glass under argon or nitrogen at atmospheric pressure. The water content of the solvent was confirmed with a Karl-Fischer Moisture Titrator (MKC-210, Kyoto Electronics Company) to be <100 ppm. Distilled water was further purified with Millipore Milli-Q. Bath sonication for dispersion of CNHs was carried out on a Honda Electronics WT-200-M instrument. Powder X-ray diffraction (PXRD) measurements were carried out on Rigaku SmartLab. Dynamic laser light scattering (DLS) was carried out on a Malvern Zetasizer Nano ZS machine. pH measurement was carried out by using pH paper (UNIV, Toyo Roshi Co. Ltd) or HORIBA pH metre D-51 with an electrode 9680 S. FE-SEM observation was carried out on a FEI Magellan 400 L operating with acceleration voltage of 1 kV and beam current of 1.3–25 pA. The size analysis of TEM and SEM images was conducted by ImageJ 1.47t software.

Xing J., Schweighauser L., Okada S., Harano K, & Nakamura E. (2019). Atomistic structures and dynamics of prenucleation clusters in MOF-2 and MOF-5 syntheses. Nature Communications, 10, 3608.

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Characterization of Lipid Nanoparticle Formulations

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DNA or mRNA concentration in LNP formulations was determined using a NanoDrop Spectrophotometer (Thermo Fisher Scientific). To calculate mRNA encapsulation efficiency within LNPs, a modified Quant-iT RiboGreen RNA assay (Thermo Fisher Scientific, R11490) was used as previously described²⁶. LNP hydrodynamic diameter and polydispersity (PDI) were measured using a Zetasizer Nano ZS machine (Malvern Instrument). For analysis of LNP structure using cryogenic-transmission electron microscopy (Cryo-TEM), LNP samples were prepared in a vitrification system (25°C, ~100% humidity). A 3 μL sample of LNP solution was dropped on a lacey copper grid coated with a continuous carbon film and blotted to remove excess sample without damaging the carbon layer. A grid was mounted on a Gatan 626 single tilt cryogenic-holder equipped in the TEM column. Images of LNP samples were recorded on an UltraScan 1000 CCD camera (Gatan).

Guimaraes P.P., Zhang R., Spektor R., Tan M., Chung A., Billingsley M.M., El-Mayta R., Riley R.S., Wang L., Wilson J.M, & Mitchell M.J. (2019). Ionizable lipid nanoparticles encapsulating barcoded mRNA for accelerated in vivo delivery screening. Journal of controlled release : official journal of the Controlled Release Society, 316, 404-417.

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Physicochemical Characterization of Hydrocortisone Nano-Formulations

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The following chemicals were sourced and utilized throughout this study: acetone, ethanol, methanol, Hydrocortisone (HCT), sorbitan monostearate (SMS), polysorbate 80 (Tween 80), distilled water (dH₂O), hydrochloric acid (HCl), stearic acid, oleic acid, sodium hydroxide (NaOH), Carboxymethyl Cellulose (CMC), Trypan blue dye, polycaprolactone (PCL), phosphate buffered saline (PBS), Dulbecco’s Modified Eagle’s Medium (DMEM), Trypsin, Dimethyl sulfoxide (DMSO), dimethylimidazole diphenyl tetrazolium bromide (MTT), and mannitol. All chemicals utilized were sourced from Sigma Aldrich (St. Louis, MO, USA) and were of high analytical and experimental grade.
The following equipment was sourced and utilized throughout this study: nanophotometer (Implen^™ NanoPhotometer^™ NP80 UV/Vis Spectrophotometer (Munich, Germany)), DSC (Mettler Toledo, DSC1, STARe Instrument (Schwerzenback, Switzerland)], FTIR [PerkinElmer Spectrum 100, Beaconsfield, UK) TGA [PerkinElmer TGA 4000, Beaconsfield, UK], Zeta sizer [Zeta-sizer Nano-ZS machine (Malvern Instruments, Worcestershire, UK)), sonicator (Scientech Ultrasonic Cleaner, Labotec, South Africa], lyophilizer (FreeZone^® 2.5, Labconco^®, Kansas City, MS, USA), incubator (RS Biotechnological Galaxy, Irvine, UK), and inverted light microscope, Model CKX53 (Olympus, Tokyo, Japan).

Kondiah P.P., Rants’o T.A., Mdanda S., Mohlomi L.M, & Choonara Y.E. (2022). A Poly (Caprolactone)-Cellulose Nanocomposite Hydrogel for Transdermal Delivery of Hydrocortisone in Treating Psoriasis Vulgaris. Polymers, 14(13), 2633.

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Nanoparticle Size Characterization by DLS

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Measurements were made on a Zetasizer Nano ZS machine (Malvern Instruments). Dispersion technology software (Malvern Instruments) with a standard setting was used for data analysis.

Jiang D., Ge Z., Im H.J., England C.G., Ni D., Hou J., Zhang L., Kutyreff C.J., Yan Y., Liu Y., Cho S.Y., Engle J.W., Shi J., Huang P., Fan C., Yan H, & Cai W. (2018). DNA origami nanostructures can exhibit preferential renal uptake and alleviate acute kidney injury. Nature biomedical engineering, 2(11), 865-877.

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Physicochemical Characterization of OEA-SPC Nanoparticles

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The OEA–SPC NPs was analysed using XRD (Phillips X’pert Pro Super), FTIR (Bruker IFS-55 FTIR spectrometer), and H¹NMR (AVANCE III 600 MHz). The bulk OEA powers, SPC, and the physical mixture of OEA and SPC were used as control. Morphology of the OEA–SPC NPs was examined by SEM (UV-70) and TEM (JEM-2100) at 5 and 200 kV, respectively. The Size and zeta-potential values were determined by a Malvern Zetasizer Nano-ZS machine (Malvern Instruments, Malvern). Three parallel measurements were carried out to determine the average values. The content of OEA in OEA–SPC NPs was determined by LC–MS (3200Qtrap). The content efficiency was calculated by Eq. (1):

Drug loading content of OEA (%) = (weight of OEA in NPs) / (weight of NPs) \times 100 %

Yang X., Xu L., Zhou J., Ge Y., Wu S., Huang J., Li Y., Zhu M., Jin X, & Yang L. (2019). Integration of phospholipid-complex nanocarrier assembly with endogenous N-oleoylethanolamine for efficient stroke therapy. Journal of Nanobiotechnology, 17, 8.

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Zeta Potential Measurement Protocol

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ζ potential measurements
were performed on a Malvern ZetaSizer Nano ZS machine, which measures
mobilities by means of laser Doppler microelectrophoresis. Since the
measurements involved inorganic solvents, a Malvern “dip cell”
probe and a fused glass cuvette were employed. Mobility measurements
consisted of 50–100 runs at a temperature of 25 °C and
a voltage of 40 V. For data acquisition, we used Malvern ZetaSizer
software version 5.1.

Kamp M., de Nijs B., van der Linden M.N., de Feijter I., Lefferts M.J., Aloi A., Griffiths J., Baumberg J.J., Voets I.K, & van Blaaderen A. (2020). Multivalent Patchy Colloids for Quantitative 3D Self-Assembly Studies. Langmuir, 36(9), 2403-2418.

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Characterization of Au-Cys-MTX/DOX Nanocomposites

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The structure of TRCys-MTX and TRCys-MTX/DOX were analyzed by H¹NMR (AVANCE III 600 MHz). Morphology of the Au-Cys-MTX/DOX NCs and Au NCs was examined by SEM (UV-70) at 10 kV and TEM (Tecnai G2 Spirit) at 20 kV. The energy spectrum analysis of Au-Cys-MTX/DOX NCs and Au NCs was also examined by UV-70. The Size and zeta-potential values were determined by a Malvern Zetasizer Nano-ZS machine (Malvern Instruments, Malvern). Three parallel measurements were carried out to determine the average values. The content of Cys-MTX/DOX in Au-Cys-MTX/DOX NCs was determined by thermogravimetry analysis (SDT_Q 600) and ultraviolet spectrophotometry (Cary5000).

Wu S., Yang X., Luo F., Wu T., Xu P., Zou M, & Yan J. (2018). Biosynthesis of flower-shaped Au nanoclusters with EGCG and their application for drug delivery. Journal of Nanobiotechnology, 16, 90.

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Nanoparticle Characterization by Zetasizer

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The size, stability, and polydispersity index (PdI) the nanoparticles were determined using a Zeta sizer Nano-ZS machine (Malvern Instruments, Worcestershire, UK). Briefly, 5 mg of each lyophilized sample was dissolved in distilled water and filtered through 0.22 µm syringe filter (Millipore Corp., Bedford, MA, USA). The samples were transferred to cuvettes and sonicated to ensure complete dissolution. The analyses were performed in triplicates. All readings were performed at 25 °C. Results were obtained using dynamic light scattering and recorded [42 (link),43 (link)].

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