SN38, Hoechst 33258, cholesterol, Pluronic F68, rhodamine B, and phosphotungstic acid were purchased from Sigma-Aldrich (St Louis, MO, USA). DSPE (1,2-distearoyl-sn-glycero-3-phosphoethanolamine and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine–N-(folate[polyethylene glycol 2,000) (PEG2,000 folate) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Epikuron 200 was obtained from Lucas Meyer (Champlan, France). Sodium hydroxide, phosphoric acid, disodium hydrogen phosphate dehydrate, and sodium dihydrogen phosphate monohydrate were obtained from Merck (Darmstadt, Germany). Methanol, ethanol, and acetonitrile of analytical reagent grade were obtained from Mallinckrodt (Staines-upon-Thames, UK). The human breast adenocarcinoma cell line MCF7 was purchased from the Culture Collection and Research Center of the Food Industry Research and Development Institute (Hsinchu, Taiwan).
Ethanol
Manufactured by Mallinckrodt
Sourced in United States, United Kingdom
Ethanol is a clear, colorless liquid that is commonly used as a laboratory reagent. It has a distinct alcoholic odor and serves as a solvent, disinfectant, and fuel source in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Methanol, by Mallinckrodt (6 mentions)
Acetonitrile, by Mallinckrodt (6 mentions)
Ethyl acetate, by Mallinckrodt (3 mentions)
Acetone, by Mallinckrodt (3 mentions)
Sodium hydroxide (naoh), by Merck Group (2 mentions)
Cholesterol, by Merck Group (2 mentions)
Phosphoric acid, by Merck Group (2 mentions)
Rhodamine b, by Merck Group (2 mentions)
Sodium dihydrogen phosphate monohydrate, by Merck Group (2 mentions)
Hexane, by Mallinckrodt (2 mentions)
Propylene glycol, by Merck Group (2 mentions)
Trichloroacetic acid, by Merck Group (2 mentions)
Thiobarbituric acid, by Merck Group (2 mentions)
Polyvinyl alcohol (pva), by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
N n diisopropylethylamine, by Merck Group (2 mentions)
N boc ethylenediamine, by Merck Group (2 mentions)
Quercetin, by Merck Group (2 mentions)
Pluronic f68, by Merck Group (1 mentions)
Disodium hydrogen phosphate dehydrate, by Merck Group (1 mentions)
12 protocols using ethanol
1
Optimization of Lipid-Based Nanoparticles
2
Liposomal Formulation Development
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Belinostat was purchased from Active Biochem (HK). The 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) and 1,2-dioleoyl-sn-glycero-3-phospho-L-serine (DOPS) were obtained from Avanti Polar Lipids (Alabaster, AL, USA). Carbamazepine, cholesterol, rhodamine B, and uranyl acetate were purchased from Sigma-Aldrich (St. Louis, MO, USA). Sodium phosphate dibasic (Na2HPO4) and citric acid were obtained from Merck (Darmstadt, Germany). Ethanol, chloroform, and acetonitrile of analytical reagent grade were obtained from Mallinckrodt (Staines-upon-Thames, UK).
3
Analysis of Methyltransferase Inhibitors
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AdoMet, S-(59-adenosyl)-L-homocysteine (AdoHcy), adenosine-29,39-dialdehyde (Adox), COMT, [ 13 C 16 ]-PA, tolcapone, 3,4-dihydroxyacetophenone (DHAP), 39-hydroxy-49-methoxyacetophenone (3H4MAP), 49-hydroxy-39-methoxyacetophenone (4H3MAP), N-tris(hydroxymethyl)-methyl-2-aminoethane sulphonic acid, methyl pentadecanoate, PAME, and losartan were purchased from Sigma-Aldrich (St. Louis, MO). Ethyl acetate, ethanol, and hexane were purchased from Mallinckrodt (St. Louis, MO).
4
Formulation and Characterization of Paclitaxel Delivery
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BRIJ 97 (polyoxyethylene 10 oleoyl ether) and propylene glycol were obtained from Sigma-Aldrich Co. (St Louis, MO, USA). Monocaprylin was kindly supplied by Abitec Corporation (Janesville, WI, USA), and myvacet oil (diacetylated monoglycerides from soybean oil) was obtained from Quest (Norwich, NY, USA). Acetonitrile, ethanol, and methanol were purchased from Mallinckrodt Baker (Phillipsburg, NJ, USA), and paclitaxel from Polymed Therapeutics (Houston, TX, USA). Penetratin, transportan, and TAT were purchased from Anaspec (Fremont, CA, USA).
5
Antioxidant Compound Synthesis Protocol
6
Preparation of Biodegradable Polymer Nanoparticles
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PLGA 50:50, molecular weight 38,000 to 54,000 g/mol, polyvinyl alcohol (PVA) 87-89% hydrolyzed (31,000-50,000 g/mol), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo[4,5-b]pyridinium 3-oxid hexafluorophosphate), N-Boc-ethylenediamine, N,N-diisopropylethylamine, trifluoroacetic acid (TFA), triethylamine, TRITC and dichloromethane (DCM) were purchased from Sigma-Aldrich (MO, USA). Chitosan (MW 100,000-300,000 g/mol) was purchased from Fisher Scientific. Acetone, ethanol, ethyl acetate were HPLC grade (Mallinckrodt Baker, NJ, USA).
7
Antioxidant Activity Evaluation
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Quercetin, BHT, 2,2′-diphenyl-1-picrylhydrazyl radical (DPPH), dimethyl sulfoxide (DMSO), thiobarbituric acid, trichloroacetic acid, hydrochloric acid, and 1,1,3,3-tetraethoxypropane used in the present study were obtained from Sigma-Aldrich (St. Louis, MO, USA). A food grade Spanish extra virgin olive oil (EVO) was used (Kuali® trademark, Monterrey, NL, Mexico), while methanol and ethanol were of liquid chromatography and spectrophotometry grade (Mallinckrodt Baker Inc., Mexico City, Mexico). Deionized water (MontRial, San Luis Potosi, Mexico) was used for aqueous solutions.
8
Multifunctional PLGA-Chitosan Nanoparticles
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PLGA (or poly (lactic-co-glycolic) acid at 40–75 g/mol, used 50:50), chitosan (Chi) (100–300 g/mol), polyvinyl alcohol (PVA) (31–50 g/mol) 87–89% hydrolyzed, fluorescein isothiocyanate (FITC), N-Boc-ethylenediamine, N,N-diisopropylethylamine (DIPEA),trifluoroacetic acid (TFA), 1-[Bis(dimethylamino)methylene]-1H-1,2,3-triazolo [4,5-b]pyridinium 3-oxid hexafluorophosphate) (HATU), 4′,6′-diamidino-2-phenylindole (DAPI), boric acid, and potassium iodine were acquired from Sigma-Aldrich (St. Louis, MO, USA). HPLC-grade ethyl acetate, acetone, DCM, ethanol, and acetonitrile were purchased from Mallinckrodt Baker (Pittsburgh, NJ, USA).
9
Milk, Soy, and Analytical Reagents
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Whole milk (4% protein and 4.5% fat) was purchased from the local market. Defatted soy flour (5.6% moisture, 75% protein); gum acacia (food grade) was supplied by Danisco India Pvt. Ltd.; ethanol, methanol, and acetonitrile (analytical grade, J.T. Baker, France), hexane (analytical grade; Mallinckrodt Chemicals; Saint Louis, USA), de-Ionized water (HPLC-grade) maltodextrin DE 18 were supplied by Danisco India Pvt. Ltd.
10
Antioxidant and Cytotoxicity Assay Protocol
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Methanol (MeOH), hydrochloric acid (HCl), sodium carbonate, sodium sulphate, acetone, phosphate buffered saline (PBS) were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Folin-Ciocalteu reagent, quercetin, ascorbic acid, ferulic acid, chlorogenic acid, caffeic acid, p-coumaric acid and syringic acid, xanthophyll, zeaxanthin, β-cryptoxanthin, dichlorofluorescein- diacetate (DCFH-DA), were purchased from Sigma (St. Louis, MO). 2, 2-Azobis-amidinopropane (ABAP) was purchased from Wako Chemicals (Richmond, VA). Gallic acid was purchased from ICN Biomedical Inc. (Costa Mesa, CA). Ethyl acetate, triflouroacetic acid, and ethanol were purchased from Mallinckrodt (Paris, KS). Sodium hydroxide, hexane, acetonitrile, magnesium carbonate, tetrahydrofuran were obtained from Fisher Scientific (Pittsburgh, PA). MDA human breast cancer cell lines and HepG2 liver cancer cell lines are provided by the American Type Culture Collection (ATCC, Rockville, MD). Williams’ medium E (WME), α-MEM, Hanks’ Blanced Salt Solution (HBSS) were purchased from Gibco Life Technologies, and Fetal bovine serum (FBS) was purchased from Atlanta Biologicals (Lawrenceville, GA).
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