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Dental cement is a material used in various dental procedures to secure and stabilize dental restorations, such as crowns, bridges, and inlays. It functions as an adhesive agent, binding the restoration to the underlying tooth structure.

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Lab products found in correlation

Millmax, by Dentsply (3 mentions) Heating pad, by Harvard Apparatus (3 mentions) 33g beveled needle, by World Precision Instruments (2 mentions) Absorptive spears, by Fine Science Tools (2 mentions) Liquid mold rubber, by Smooth-On (2 mentions) C b metabond, by Parkell (2 mentions) Nanoinjector 2, by Drummond (2 mentions) Xylazine, by Merck Group (1 mentions) Ketamine hcl, by Merck Group (1 mentions) Stereotaxic frame, by Kopf Instruments (1 mentions) Methyl methacrylate liquid, by Dentsply (1 mentions) Loctite 401, by Henkel Adhesive Technologies (1 mentions) Viscotears, by Alcon (1 mentions) Medetomidine, by Orion Pharma (1 mentions) Stereotaxic apparatus, by Kopf Instruments (1 mentions) Hd s02, by Data Sciences International (1 mentions) Meloxicam, by Boehringer Ingelheim (1 mentions) Guide cannula, by Bioanalytical Systems (1 mentions)

19 protocols using dental cement

1

In Vivo Ca2+ Imaging Using GRIN Lens

Cited 2 times
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For in vivo Ca2+ imaging, mice underwent a single surgery in which 400 nl of AAV8-CamkII-GCamp6f virus were injected unilaterally with a 10 μl NanoFil syringe and a 33g beveled needle (World Precision Instruments, Sarasota, FL) at a constant speed of 100 nl/min prior to implanting a GRIN lens over the injection site. GRIN lenses were 6.1 mm long with a 0.5 mm diameter for vDG imaging (Inscopix, Palo Alto, CA). GRIN lens implantation was performed as previously described27 (link),32 (link). Briefly, a craniotomy centered at the lens implantation site was made and dura was carefully removed from the brain surface and cleaned with a stream of sterile saline and absorptive spears (Fine Science Tools, Foster City, CA). No brain tissue was aspirated prior to lowering the GRIN lens. Three 1/16” microscrews (Antrin Miniature Specialities, Inc) were inserted in evenly spaced locations around the implantation site. The lens was lowered in 0.1 mm DV steps and then fixed to the skull with dental cement (Dentsply, Sinora, PA). Viral injections coordinates were −3.6 mm AP, ±2.8 mm ML, −3.2 mm DV (from brain). Lens placement coordinates were −3.6 mm AP, ±2.8 mm ML, −2.9 mm DV (from brain). At the completion of surgery, the lens was protected with liquid mold rubber (Smooth On, Lower Macungie, PA), and imaging experiments commenced 4 weeks later.
Anacker C., Luna V.M., Stevens G., Millette A., Shores R., Jimenez J.C., Chen B, & Hen R. (2018). Hippocampal neurogenesis confers stress resilience by inhibiting the ventral dentate gyrus. Nature, 559(7712), 98-102.
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2

Surgical Implantation of EEG and EMG Electrodes in Mice

Cited 1 time
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Surgical and implantation procedures were performed as previously described (Kam et al., 2016 (link)). Mice were anesthetized continuously with inhaled isoflurane and placed in a stereotaxic apparatus (David Kopf). After exposing the skull, five electrodes were positioned. Two subdural electrodes (2.5 mm diameter screws with tapered tips, Pinnacle Technologies), symmetrically placed over left and right primary motor cortices (1.5 mm anterior to Bregma, ± 2.0 mm lateral to the midline) served as EEG electrodes. Two epidural screw electrodes were placed above the cerebellum to serve as reference and ground. A bipolar, twisted stainless steel electrode (California Fine Wire Co.) inserted into the nuchal muscles served as an electromyogram (EMG) site. After implantation, a 6-pin connector (Mill-max) was centered over the skull with dental cement (Dentsply) and the animal was placed in its home cage on top of a heating pad set to 37°C (Harvard Apparatus) until fully ambulatory. All animals were supplied with subcutaneous hydration and pain control (buprenorphine) following surgery.
Pettibone W.D., Kam K., Chen R.K, & Varga A.W. (2019). Necessity of Sleep for Motor Gist Learning in Mice. Frontiers in Neuroscience, 13, 293.
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3

In Vivo Ca2+ Imaging Using GRIN Lens

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vivo Ca2+ imaging, mice underwent a single surgery in which 400 nl of AAV8-CamkII-GCamp6f virus were injected unilaterally with a 10 μl NanoFil syringe and a 33g beveled needle (World Precision Instruments, Sarasota, FL) at a constant speed of 100 nl/min prior to implanting a GRIN lens over the injection site. GRIN lenses were 6.1 mm long with a 0.5 mm diameter for vDG imaging (Inscopix, Palo Alto, CA). GRIN lens implantation was performed as previously described27 (link),32 (link). Briefly, a craniotomy centered at the lens implantation site was made and dura was carefully removed from the brain surface and cleaned with a stream of sterile saline and absorptive spears (Fine Science Tools, Foster City, CA). No brain tissue was aspirated prior to lowering the GRIN lens. Three 1/16” microscrews (Antrin Miniature Specialities, Inc) were inserted in evenly spaced locations around the implantation site. The lens was lowered in 0.1 mm DV steps and then fixed to the skull with dental cement (Dentsply, Sinora, PA). Viral injections coordinates were −3.6 mm AP, ±2.8 mm ML, −3.2 mm DV (from brain). Lens placement coordinates were −3.6 mm AP, ±2.8 mm ML, −2.9 mm DV (from brain). At the completion of surgery, the lens was protected with liquid mold rubber (Smooth On, Lower Macungie, PA), and imaging experiments commenced 4 weeks later.
Anacker C., Luna V.M., Stevens G., Millette A., Shores R., Jimenez J.C., Chen B, & Hen R. (2018). Hippocampal neurogenesis confers stress resilience by inhibiting the ventral dentate gyrus. Nature, 559(7712), 98-102.
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4

Implantation of EEG/EMG Electrodes in Mice

Cited 1 time
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Implantation of EEG/EMG electrodes were performed as previously described (Kam, Duffy et al. 2016 (link)). Mice were anesthetized with inhaled isoflurane and placed in a stereotaxic apparatus (David Kopf). After exposing the skull, 5 electrodes were positioned. Two subdural electrodes (2.5 mm diameter screws with tapered tips, Pinnacle Technologies), symmetrically placed over left and right primary motor cortices (1.5 mm anterior to Bregma, ± 2.0 mm lateral to the midline) served as EEG electrodes. Two epidural screw electrodes were placed above the cerebellum to serve as reference and ground. A bipolar, twisted stainless steel electrode (California Fine Wire Co.) inserted into the nuchal muscles served as an electromyogram (EMG) site. After implantation, a 6-pin connector (Millmax) was centered over the skull with dental cement (Dentsply) and the animal was placed in its home cage on top of a heating pad set to 37°C (Harvard Apparatus) until fully ambulatory. All animals were supplied with subcutaneous hydration and pain control (buprenorphine) following surgery.
Kam K., Pettibone W.D., Shim K., Chen R.K, & Varga A.W. (2019). Dynamics of Sleep Spindles and Coupling to Slow Oscillations Following Motor Learning in Adult Mice. Neurobiology of learning and memory, 166, 107100.
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5

Stereotaxic Electrode Implantation for Neural Recordings

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Electrodes were designed and constructed in-house and were similar to those used in our previous publications [26 (link)–28 (link)]. Animals were anesthetized with isoflurane gas (4% induction, 2% maintenance) and mounted in a stereotaxic frame. Custom electrodes were implanted bilaterally targeting the PL mPFC (from bregma: DV −4 mm; AP +3.4 mm; ML ±0.75 mm), IL mPFC (from bregma: DV −5 mm; AP +3.4 mm; ML ±0.75 mm), NAc shell (from bregma: DV −8 mm; AP +1.2 mm; ML ±1.0 mm), and CA1 of the hippocampus (from bregma: DV −2.5 mm; AP −3.8 mm; ML ±2.5 mm). Four stainless steel skull screws were placed around the electrode site and dental cement (Dentsply) was applied to secure the electrodes in place. Rats were allowed to recover for at least 7 days before any experimentation began.
Henricks A.M., Sullivan E.D., Dwiel L.L., Li J.Y., Wallin D.J., Khokhar J.Y, & Doucette W.T. (2022). Maternal immune activation and adolescent alcohol exposure increase alcohol drinking and disrupt cortical-striatal-hippocampal oscillations in adult offspring. Translational Psychiatry, 12, 288.
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6

In Vivo Calcium Imaging of Dorsal CA1

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Mice underwent two surgical procedures under isoflurane (1%-2% vol/vol). We injected 500 nL of a 1:3 dilution in PBS of AAV2/1 serotype virus expressing GCaMP6f under the control of the CaMKII promoter (UPENN Vector Core, AAV1.CamKII.GCaMP6f.WPRE.SV40, titer 1-3 × 10 13 vg/ml) with a thin glass pipette into the left hemisphere of dorsal CA1 (-2.2 mm from bregma, 1.6 mm mediolateral, -1.2 mm dorsoventral). 1-2 weeks after viral injection we implanted a 1.8 mm diameter imaging cannula (metal cannula with a glass coverslip attached at the bottom, Inscopix part number 1050-002189) over the dorsal surface of CA1 centered on the site of viral injection after aspiration of the overlying cortical area as previously described (Barretto et al., ). We then secured the cannula and a custom metal head bar to the cranium of the mice using dental cement (Dentsply). 1-2 weeks after cannula implantation we inserted a 1 mm diameter gradient refractory index (GRIN) micro-endoscope (Inscopix part number 1050-002176) into the cannula and a plastic baseplate (Inscopix part number 1050-002192) was cemented into place after confirming even expression of GCaMP6f in healthy tissue using a miniaturized fluorescent microscope (Inscopix nVista, v2.0).
Fischler-Ruiz W., Clark D.G., Joshi N.R., Devi-Chou V., Kitch L., Schnitzer M., Abbott L.F, & Axel R. (2021). Olfactory landmarks and path integration converge to form a cognitive spatial map. Neuron, 109(24).
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7

Intracerebral CNiFER Cell Implantation

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CNiFERs were harvested without trypsin from 80 % confluent culture flasks, centrifuged, and re-suspended in ACSF for injection. For the in vivo dose response experiment, a open craniotomy was used. For all other in vivo experiments, a ‘thinned skull’ craniotomy34 (link) was used. CNiFER cells were loaded into a 40 μm inner-diameter glass pipette connected to a Nanoinjector II (Drummond) and injected into neocortex through the thinned skull ~200 μm from the cortical surface. CNiFERs were injected into adjacent sites within the following stereotaxic coordinates: +1 to +2 mm A/P; +1 to +2 mm M/L. After implantation in several adjacent sites (typically two injection sites per CNiFER variant), the craniotomy was sealed with a glass coverslip. A custom-built head-bar was attached to the skull with C&B-METABOND (Parkell, Inc.), and the preparation surrounding the imaging window was covered with dental cement (Dentsply). Mice were immunosuppressed by daily cyclosporine injection (20 μl/100 g, i.p., Belford Laboratories).
Muller A., Joseph V., Slesinger P.A, & Kleinfeld D. (2014). Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex. Nature methods, 11(12), 1245-1252.
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8

Intracerebral CNiFER Cell Implantation

Check if the same lab product or an alternative is used in the 5 most similar protocols
CNiFERs were harvested without trypsin from 80 % confluent culture flasks, centrifuged, and re-suspended in ACSF for injection. For the in vivo dose response experiment, a open craniotomy was used. For all other in vivo experiments, a ‘thinned skull’ craniotomy34 (link) was used. CNiFER cells were loaded into a 40 μm inner-diameter glass pipette connected to a Nanoinjector II (Drummond) and injected into neocortex through the thinned skull ~200 μm from the cortical surface. CNiFERs were injected into adjacent sites within the following stereotaxic coordinates: +1 to +2 mm A/P; +1 to +2 mm M/L. After implantation in several adjacent sites (typically two injection sites per CNiFER variant), the craniotomy was sealed with a glass coverslip. A custom-built head-bar was attached to the skull with C&B-METABOND (Parkell, Inc.), and the preparation surrounding the imaging window was covered with dental cement (Dentsply). Mice were immunosuppressed by daily cyclosporine injection (20 μl/100 g, i.p., Belford Laboratories).
Muller A., Joseph V., Slesinger P.A, & Kleinfeld D. (2014). Cell-based reporters reveal in vivo dynamics of dopamine and norepinephrine release in murine cortex. Nature methods, 11(12), 1245-1252.
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9

Neurophysiological Recording of Auditory Responses

Cited 1 time
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Methods for neurophysiological recording are as described in Phan et al. (2006 (link)); Phan and Vicario (2010 (link)); Tsoi et al. (2014 (link)) and Bell et al. (2015 (link)). Briefly, the subject underwent surgical preparation for the neural recording prior to the presentation of auditory stimuli used for electrophysiological assessment of evoked responses to sound. Each bird was anesthetized (1.5%–2.0% isoflurane in oxygen), and surgically implanted with a head fixation pin and recording chamber over a craniotomy centered over the auditory forebrain using dental cement (Dentsply Caulk, Milford, DE, USA). A motorized microdrive (Eckhorn, Thomas Recording, Giessen, Germany) was used to advance independently 16 tungsten microelectrodes (quartz platinum/tungsten, impedance: 2–4 MΩ, Thomas Recording) bilaterally into the brain, targeting areas NCM (four electrodes per hemisphere) and the Field L complex (four electrodes per hemisphere; consisting of L1, L2a and L3; referred to as Non-NCM in the text). Both areas were defined prior to electrode placement from fiduciary landmarks centered on the bifurcation of the midsagittal sinus. Additionally, NCM and non-NCM areas were located electrophysiologically by their characteristic response patterns to white noise search stimuli shaped with the amplitude envelope of zebra finch song.
Phan M.L., Gergues M.M., Mahidadia S., Jimenez-Castillo J., Vicario D.S, & Bieszczad K.M. (2017). HDAC3 Inhibitor RGFP966 Modulates Neuronal Memory for Vocal Communication Signals in a Songbird Model. Frontiers in Systems Neuroscience, 11, 65.
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10

Bilateral VTA Cannula Implantation in Rats

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Prior to surgery, rats were anesthetized with ketamine HCL (100 mg/kg, i.p., Sigma Aldrich, USA) and xylazine (10 mg/kg, i.p., Sigma Aldrich, USA). Rats were then placed in a stereotaxic frame (David Kopf Instruments, Tujunga, CA, USA) for intra-cranial cannula implantation. Coordinates were obtained from the rat brain atlas [28 ] with anteroposterior (AP), mediolateral (ML), and dorsoventral (DV) positions referenced from Bregma. A bilateral cannula apparatus with cannula spaced 1 mm apart (Plastics One, Roanoke, VA, USA) was placed 1 mm above the VTA (AP-5.2 mm, ML ±0.5 mm, DL −7.0 mm from dura) and secured to the skull using screws (Gexpro, High Point, NC) and dental cement (Dentsply, Milford, DE, USA). After surgery, rats were singly housed and allowed to recover for 5–7 days before behavioral testing. Animal protocols were approved by Yale University Institutional Animal Care and Use Committee (IACUC) and performed in accordance with the National Institutes of Health (NIH) Guide for the Care and Use of Laboratory Animals.
Small K.M., Nunes E., Hughley S, & Addy N.A. (2016). Ventral tegmental area muscarinic receptors modulate depression and anxiety-related behaviors in rats. Neuroscience letters, 616, 80-85.
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