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Multiplex platform

Manufactured by Merck Group
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The Multiplex platform is a versatile laboratory equipment designed for high-throughput analysis and quantification of multiple analytes simultaneously. It enables the detection and measurement of various biomolecules, such as proteins, nucleic acids, or small molecules, within a single sample. The core function of the Multiplex platform is to facilitate efficient and comprehensive analysis, providing researchers and scientists with a powerful tool for their analytical needs.

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Lab products found in correlation

Analyst software, by Merck Group (4 mentions) Er tracker, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

Luminex Multiplex platform (9 citations) Luminex xMap platform multiplex assay (2 citations) HSP2MAG-63K multiplex bead platform (2 citations)

10 protocols using multiplex platform

1

Burned Patient Metabolism and Inflammation

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Patients admitted to the Ross Tilley Burn Centre at Sunnybrook Hospital or patients undergoing elective surgery were consented pre-operatively for tissue collection. Approval for our study was obtained from the Research Ethics Board at Sunnybrook Hospital. We enrolled 20 severely burned adults (46.3 ± 3.9 years old; 14 males and 6 females) with burns encompassing 48% ± 3.9% of their total body surface area (TBSA). Fat obtained at first OR (less than 3 days) and last OR (greater than 10 days) from patients were immediately transferred to the laboratory and either frozen at −80°C until further analysis or transferred in fixative. MREE was measured as previously described (Jeschke et al., 2011 (link)). Propranolol was administrated according to standard dosing and protocols in order to decrease heart rate below 100 bpm. IL-6 in plasma was determined using a multiplex platform (Millipore) in accordance with manufacturer’s protocol.
Patsouris D., Qi P., Abdullahi A., Stanojcic M., Chen P., Parousis A., Amini-Nik S, & Jeschke M.G. (2015). Burn Induces Browning of the Subcutaneous White Adipose Tissue in Mice and Humans. Cell reports, 13(8), 1538-1544.
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2

Multiplex Analysis of Inflammatory Cytokines

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Three inflammatory cytokines (IL-6, IL-1β, IL-10) were analyzed using the Multiplex platform (Millipore, MA) according to the manufacturer’s protocol. Raw data were processed using Millipore Analyst software. Cytokine concentrations are denoted as mean ± SEM, expressed in pg/mL.
Qi P., Abdullahi A., Stanojcic M., Patsouris D, & Jeschke M.G. (2016). Lipidomic analysis enables prediction of clinical outcomes in burn patients. Scientific Reports, 6, 38707.
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3

Plasma Biomarker Analysis in Mice

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Rodent EDTA-anticoagulated blood samples were collected from all mice at the time of sacrifice and stored in −80°C until analysis. Blood samples were centrifuged for 20 minutes at 3,000 rpm at 4 °C. Plasma samples were used to compare inflammatory, chemokine and immune mediators between groups using a Multiplex platform (Millipore, MA). Experimental kits were all conducted in accordance with manufacturers’ protocol. Raw data was processed using Millipore Analyst software. All values are presented as mean ± SEM and expressed in pg/ml.
Vinaik R., Stanojcic M, & Jeschke M.G. (2018). NLRP3 Inflammasome Modulates Post-Burn Lipolysis and Hepatic Fat Infiltration via Fatty Acid Synthase. Scientific Reports, 8, 15197.
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4

Cytokine Profiling in Rodent and Human Sera

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Rodent and human sera, as well as macrophage-conditioned medium, were collected and using a Multiplex platform (Millipore, MA) and cytokine profiles were measured.
Abdullahi A., Auger C., Stanojcic M., Patsouris D., Parousis A., Epelman S, & Jeschke M.G. (2019). Alternatively Activated Macrophages Drive Browning of White Adipose Tissue in Burns. Annals of surgery, 269(3), 554-563.
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5

Immunofluorescence and Cytokine Analysis

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Cells on eight chamber slides were fixed in paraformaldehyde, blocked with 1% serum bovine serum albumin in PBS and were stained with antibodies against cleaved-ATF6, BiP at a dilution ratio of 1:200 overnight. Slides were washed and incubated for 1 hr at room temperature with the appropriate secondary antibodies (each at a dilution factor of 1:500). The ER tracker used was from Invitrogen and used according to the manufacturers protocol. Slides were washed and counterstained with DAPI before mounting. Slides were stored in 4°C. Slides were imaged using a Zeiss spinning disk confocal microscope. Cytokine/inflammatory profile. Rodent sera was collected and utilized to compare inflammatory profiles between control, thapsigargin and tunicamycin treated samples. Using the Multiplex platform (Millipore, MA), a panel of pro-inflammatory cytokines, chemokines and growth factors were all analyzed. Raw data was processed using Millipore Analyst software and all values are presented as mean ± SEM for the respective cytokine concentration, expressed in μg/ml.
Abdullahi A., Stanojcic M., Parousis A., Patsouris D, & Jeschke M.G. (2017). Modeling Acute ER stress in vivo and in vitro. Shock (Augusta, Ga.), 47(4), 506-513.
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6

Inflammatory Trajectories in Thermal Injury

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EDTA-anticoagulated samples were drawn within 96-hours post thermal injury and processed using a standard Percoll-based PBMC isolation from periphery. Plasma samples from non-septic and septic patients were used to compare anti-inflammatory trajectories for Interleukin (IL) 10 and IL-1 receptor antagonist (RA) using a Multiplex platform (Millipore, MA). Experimental kits were all conducted in accordance with manufacturers’ protocol. Raw data was processed using Millipore Analyst software. All values are presented as mean ± SEM and expressed in pg/ml
Chen P., Stanojcic M, & Jeschke M.G. (2017). The Septic Predictor Index (SPI): a novel platform to identify thermally-injured patients susceptible to sepsis. Surgery, 163(2), 409-414.
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7

Multiplex Cytokine Profiling

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Rodent and human sera were collected and using a Multiplex platform (Millipore, MA) IL-6 and IL-4 levels was measured.
Abdullahi A., Chen P., Stanojcic M., Sadri A.R., Coburn N, & Jeschke M.G. (2017). IL-6 signal from the bone marrow is required for the browning of white adipose tissue post burn injury. Shock (Augusta, Ga.), 47(1), 33-39.
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8

Burned Patient Metabolism and Inflammation

Cited 1 time
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Patients admitted to the Ross Tilley Burn Centre at Sunnybrook Hospital or patients undergoing elective surgery were consented pre-operatively for tissue collection. Approval for our study was obtained from the Research Ethics Board at Sunnybrook Hospital. We enrolled 20 severely burned adults (46.3 ± 3.9 years old; 14 males and 6 females) with burns encompassing 48% ± 3.9% of their total body surface area (TBSA). Fat obtained at first OR (less than 3 days) and last OR (greater than 10 days) from patients were immediately transferred to the laboratory and either frozen at −80°C until further analysis or transferred in fixative. MREE was measured as previously described (Jeschke et al., 2011 (link)). Propranolol was administrated according to standard dosing and protocols in order to decrease heart rate below 100 bpm. IL-6 in plasma was determined using a multiplex platform (Millipore) in accordance with manufacturer’s protocol.
Patsouris D., Qi P., Abdullahi A., Stanojcic M., Chen P., Parousis A., Amini-Nik S, & Jeschke M.G. (2015). Burn Induces Browning of the Subcutaneous White Adipose Tissue in Mice and Humans. Cell reports, 13(8), 1538-1544.
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9

Cytokine Secretion in B Cells

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ELISA was performed on supernatants from healthy donor volunteer B cells cultured in either RPMI media alone or with CD40L and anti-human IgM with or without KU55933. Soluble IL-6, TNFα, IL-10, and OPG were measured using a multiplex platform (Millipore). Soluble RANKL was measured by ELISA (PeproTech).
Mensah K.A., Chen J.W., Schickel J.N., Isnardi I., Yamakawa N., Vega-Loza A., Anolik J.H., Gatti R.A., Gelfand E.W., Montgomery R.R., Horowitz M.C., Craft J.E, & Meffre E. (2019). Impaired ATM Activation in B Cells is Associated with Bone Resorption in Rheumatoid Arthritis. Science translational medicine, 11(519), eaaw4626.
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10

Plasma Biomarker Analysis in Mice

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EDTA-anticoagulated blood samples were collected from all mice at the
time of sacrifice and stored in −80°C until analysis. Plasma
samples were used to compare inflammatory, chemokine and immune mediators
between groups using a Multiplex platform (Millipore, MA). Experimental kits
were all conducted in accordance with manufacturers’ protocol. Raw data
was processed using Millipore Analyst software. All values are presented as mean
± SEM and expressed in pg/ml.
Satokata I., Tanaka K., Miura N., Miyamoto I., Satoh Y., Kondo S, & Okada Y. (1990). Characterization of a splicing mutation in group A xeroderma pigmentosum.. Proceedings of the National Academy of Sciences of the United States of America, 87(24), 9908-9912.
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