S carbidopa

Manufactured by Merck Group

Sourced in United States

S‐(−)‐Carbidopa is a chemical compound used in laboratory settings. It is the levorotatory stereoisomer of Carbidopa, which is a medication used in combination with Levodopa for the treatment of Parkinson's disease. S‐(−)‐Carbidopa is primarily utilized as a reference standard or an analytical tool in research and development applications.

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Lab products found in correlation

L dopa, by Merck Group (3 mentions) D lys3 ghrp 6, by Phoenix Pharmaceuticals (1 mentions) 3 iodo l tyrosine 3iy, by Merck Group (1 mentions) L 3 4 dihydroxyphenylalanine l dopa, by Merck Group (1 mentions) Polyclonal antibodies against glyceraldehyde 3 phosphate dehydrogenase gapdh, by Merck Group (1 mentions) Buffers and hplc reagents, by Merck Group (1 mentions) Irdye secondary antibodies for odyssey imaging systems, by LI COR (1 mentions) Pargyline hcl, by Merck Group (1 mentions) Methamphetamine hcl, by Merck Group (1 mentions)

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Carbidopa (21 citations)

4 protocols using s carbidopa

Drosophila Strain Handling and Supplementation

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Drosophila strains used in this study are listed in the Supplementary file 2 KEY RESOURCES TABLE. Crosses listed in Supplementary file 3 were kept on standard cornmeal food supplemented with retinal (0.2 mM all-trans-retinal prior to eclosion and then 0.4 mM) at 21°C at 60% relative humidity in the dark. Female flies were sorted on cold plates at least 1 d prior to the experiments, and 4–10 day old flies were used for experiments. Additional drugs were administered by feeding with retinal containing fly food mixed with drugs. The L‐DOPA (D9628, Sigma) or L-NNA were mixed directly into melted fly food at final concentrations of 1 mg/ml or 1–100 mM, respectively. S‐(−)‐Carbidopa (C1335, Sigma) was dissolved in 1 ml of water at 10x the final concentration and mixed with 9 ml of melted fly food.

Aso Y., Ray R.P., Long X., Bushey D., Cichewicz K., Ngo T.T., Sharp B., Christoforou C., Hu A., Lemire A.L., Tillberg P., Hirsh J., Litwin-Kumar A, & Rubin G.M. (2019). Nitric oxide acts as a cotransmitter in a subset of dopaminergic neurons to diversify memory dynamics. eLife, 8, e49257.

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Rikkunshito Composition and Preparation

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Rikkunshito, powdered extract consisting of Atractylodis lanceae rhizoma (4 g, 18.6%), Ginseng radix (4 g, 18.6%), Pinelliae tuber (4 g, 18.6%), Hoelen (4 g, 18.6%), Zizyphi fructus (2 g, 9.3%), Aurantii nobilis pericarpium (2 g, 9.3%), Glycyrrhizae radix (1 g, 4.7%), and Zingiberis rhizoma (0.5 g, 2.3%) (Tsumura & Co., Tokyo, Japan) was suspended in water. l-dopa (l-3,4-dihydroxyphenylalanine methyl ester hydrochloride) and s-(−)-carbidopa (CD) were dissolved in vehicle composed of 10% dimethyl sulfoxide, 5% Tween-80 and 85% saline, all from Sigma-Aldrich. [d-Lys³]-GHRP-6 (Phoenix Pharmaceuticals, CA, USA) was dissolved in sterile saline.

Wang L., Mogami S., Karasawa H., Yamada C., Yakabi S., Yakabi K., Hattori T, & Taché Y. (2014). Preventive effect of rikkunshito on gastric motor function inhibited by l-dopa in rats. Peptides, 55, 136-144.

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Dopaminergic Drugs Feeding Assay

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3-Iodo-L-tyrosine (3-IY) (I8250, Sigma) and L-DOPA (D9628, Sigma) were mixed directly into melted fly food at final concentrations of 1.25 mg/ml and 1 mg/ml, respectively. S-(−)-Carbidopa (C1335, Sigma) was dissolved in 1 ml of water at the 10× final concentration and mixed with 9 ml of melted fly food. The Trikinetics activity monitor tubes were loaded with a plug of food at one end, with drug feeding throughout the course of the experiment.

Cichewicz K., Garren E.J., Adiele C., Aso Y., Wang Z., Wu M., Birman S., Rubin G.M, & Hirsh J. (2016). A New Brain Dopamine Deficient Drosophila and its Pharmacological and Genetic Rescue. Genes, brain, and behavior, 16(3), 394-403.

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Synthesis and Characterization of Methcathinone and Mephedrone Derivatives

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(R,S)-N-Methcathinone HCl and (R,S)-mephedrone HCl were provided by the NIDA Research Resources Drug Supply Program. Racemic 4-methlymethamphetamine HCl was synthesized as described by Davis et al. (2012) (link) from methylamine HCl and 4-methylphenylacetone purchased from Alfa Aesar (Ward Hill, MA, USA). (+)- Methamphetamine HCl, pargyline HCl, L-3,4-dihydroxyphenylalanine (L-DOPA), S-(−)-carbidopa, DA, polyclonal antibodies against glyceraldehyde 3-phosphate dehydrogenase (GAPDH), and all buffers and HPLC reagents were purchased from Sigma-Aldrich (St. Louis, MO, USA). Bicinchoninic acid protein assay kits for Western blot analysis were obtained from Pierce (Rockford, IL, USA). Polyclonal antibodies against rat TH were produced as previously described (Kuhn and Billingsley, 1987 (link)). Monoclonal antibodies against rat DAT were generously provided by Dr. Roxanne Vaughan (University of North Dakota, Grand Forks, ND, USA). IRDye secondary antibodies for Odyssey Imaging Systems were purchased from LiCor Biosciences (Lincoln, NE, USA).

Anneken J.H., Angoa-Perez M., Sati G.C., Crich D, & Kuhn D.M. (2017). Assessing the role of dopamine in the differential neurotoxicity patterns of methamphetamine, mephedrone, methcathinone and 4-methylmethamphetamine. Neuropharmacology, 134(Pt A), 46-56.

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