Nanophotometer p300

Manufactured by Implen

Sourced in Germany, United States

The NanoPhotometer P300 is a compact and versatile UV-Vis spectrophotometer designed for accurate and reliable measurement of samples with small volumes. It features a high-resolution touch screen display, intuitive software, and a robust design for use in various laboratory settings.

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Lab products found in correlation

Magna pure lc, by Roche (2 mentions) Lightcycler 480, by Roche (2 mentions) Superscript 3 first strand synthesis system for rt pcr, by Thermo Fisher Scientific (2 mentions) Sensimix sybr no rox, by Meridian Bioscience (2 mentions) Revertaid first strand cdna synthesis kit, by Thermo Fisher Scientific (2 mentions) Turbo dna free kit, by Thermo Fisher Scientific (2 mentions) Isolate 2 rna plant kit, by Meridian Bioscience (2 mentions) Tc20 automated cell counter, by Bio-Rad (1 mentions) Maxwell 16 lev simplyrna tissue kit, by Promega (1 mentions) High capacity cdna reverse transcriptase kit, by Thermo Fisher Scientific (1 mentions) Qiaamp dna blood mini kit, by Qiagen (1 mentions) T1000 thermal cycler, by Bio-Rad (1 mentions) Tri reagent, by Merck Group (1 mentions) Quantitect reverse transcription kit, by Qiagen (1 mentions) Goscript reverse transcription system, by Promega (1 mentions) Magna pure lc rna isolation kit 3 tissue, by Roche (1 mentions) Light cycler 480 rna master hydrolysis probes for rna, by Roche (1 mentions) Magna lyser, by Roche (1 mentions) Genejet plasmid miniprep kit, by Thermo Fisher Scientific (1 mentions) Rnaextm total rna isolation solution, by Generay (1 mentions)

43 protocols using nanophotometer p300

Microalgal Fatty Acid Profiles under Nutrient Manipulation

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Four sets of cultivation conditions were used to assess fatty acid profiles of microalga at different nitrogen and phosphorus concentrations: (1) Bold’s basal medium with 2.94 mM NaNO₃, 0.43 mM K₂HPO₄, and 1.29 mM KH₂PO₄ (control); (2) BBM depleted of NaNO₃ (-N); (3) BBM depleted of K₂HPO₄ and KH₂PO₄ (-P); and (4) BBM depleted of NaNO₃, K₂HPO₄, and KH₂PO₄ (-N-P). All experiments were conducted in three independent culture replicates.
The cultures were maintained in 250-mL Erlenmeyer glass flasks with 150 mL medium, under constant orbital shaking (150 rpm in ELMI Sky Line Shaker S-3L, ELMI Ltd, Latvia) for 25 or 60 days at 25 °C. The light intensity was 100 μmol photons m⁻² s⁻¹ with 16:8 h light/dark photoperiod. IMPLEN Nanophotometer P300 (Implen GmbH, Germany) was used to measure optical density at λ = 720 nm (OD₇₂₀). Cell concentrations were measured with a TC20 Automated Cell Counter (Bio-Rad Laboratories, USA). The initial cultures had OD₇₂₀ of 0.07925 corresponding to 0.01 × 10⁶ cells mL⁻¹.

Maltsev Y., Krivova Z., Maltseva S., Maltseva K., Gorshkova E, & Kulikovskiy M. (2021). Lipid accumulation by Coelastrella multistriata (Scenedesmaceae, Sphaeropleales) during nitrogen and phosphorus starvation. Scientific Reports, 11, 19818.

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Fly Brain RNA Isolation Protocol

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Twenty heads of exercising and 20 heads of non-exercising flies were collected at day 16 of the experiment (2 days after completing exercise phase) for RNA isolation. Each experiment was carried out in biological triplicates. Maxwell^® 16 LEV simplyRNA Tissue Kit (Promega GmbH, Mannheim, Germany) was used according to manufacturer instructions. Heads were kept in 200 μl of homogenization solution and then carefully ground with a sterile pestle for 2 min, then lysis buffer was added. Implen Nanophotometer P300 (Implen GmbH, Munich, Germany) was used to measure RNA concentrations. The High Capacity cDNA Reverse Transcriptase Kit (Applied Biosystems, Darmstadt, Germany) was used to obtain cDNA from purified RNA samples. Reverse transcription reaction was carried out in MJ Thermal Cycler PTC 200 (MJ Research Inc., Ramsey, MN, USA).

Berlandi J., Lin F.J., Ambrée O., Rieger D., Paulus W, & Jeibmann A. (2017). Swing Boat: Inducing and Recording Locomotor Activity in a Drosophila melanogaster Model of Alzheimer’s Disease. Frontiers in Behavioral Neuroscience, 11, 159.

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Determination of MDA Levels in Leaf Samples

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MDA level in the leaf samples was analyzed according to Heath and Packer [55 (link)]. About 100 mg of fresh leaves were ground into a fine powder in a mortar with 1.5 mL 0.1% trichloroacetic acid (TCA) and transferred into a 2 mL centrifuge tube. The ground sample was centrifuged at 13,000× g for 10 min at 4 °C. The supernatant (300 µL) was mixed with 1 mL mixture containing 0.5% (v/v) thiobarbituric acid (TBA) in 20% (w/v) TCA. The mixture was heated at 95 °C for 30 min, ice-cooled, and centrifuged at 10,000× g for 10 min. The MDA content was calculated with the formula below from the absorbance measurement of the supernatant at 532 and 600 nm wavelengths using a spectrophotometer, NanoPhotometer P300 (Implen GmbH, Munich, Germany).

The extinction coefficient of this MDA-TBA abduct at 532 nm is 155 mM⁻¹ cm⁻¹.

Mohd Amnan M.A., Teo W.F., Aizat W.M., Khaidizar F.D, & Tan B.C. (2023). Foliar Application of Oil Palm Wood Vinegar Enhances Pandanus amaryllifolius Tolerance under Drought Stress. Plants, 12(4), 785.

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Genomic DNA Extraction from Blood and Saliva

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All gDNA extractions were performed within 24 h after sample collection. The gDNA from blood and saliva samples were isolated using the isolation kit QIAamp® DNA Blood Mini Kit (QIAGEN, Germany) with a sample origin-dependent protocol. Blood-derived gDNA isolates were obtained from 1 mL of collected blood samples according to the manufacturer's protocol "DNA Purification from Blood or Body Fluids," whereas saliva-derived gDNA isolates were extracted from 2 mL of saliva-media mixture according to the modified QIAGEN Supplementary Protocol “Isolation of genomic DNA from saliva and mouthwash using the QIAamp® DNA Blood Mini Kit, Spin procedure protocol.” The purity of gDNA was evaluated based on the A260/280 absorbance ratio acquired spectrophotometrically on NanoPhotometer P300 (Implen, Germany) from 1 µl of gDNA isolates. The concentrations of gDNA were measured in duplicates (2 × 1 µl) using the Qubit 1 × dsDNA High Sensitivity (HS) Kit (Thermo Fisher Scientific, USA) (Additional File 1, Table 1). The integrity of gDNA was evaluated using the 0.8% agarose gel electrophoresis (GelRed 1:10 000, 100 V, 40 min), (Additional File 2, Fig. 1).

Kvapilova K., Misenko P., Radvanszky J., Brzon O., Budis J., Gazdarica J., Pos O., Korabecna M., Kasny M., Szemes T., Kvapil P., Paces J, & Kozmik Z. (2024). Validated WGS and WES protocols proved saliva-derived gDNA as an equivalent to blood-derived gDNA for clinical and population genomic analyses. BMC Genomics, 25, 187.

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RNA Extraction and cDNA Synthesis Workflow

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RNA was extracted as described previously [14] using TRI Reagent (Sigma) following the manufacturer's guidelines and quantified using a NanoPhotometer P300 (Implen). 260/280 absorbance ratios, as an index of RNA purity, were consistently >2 and RNA integrity (RIN numbers) were >7.5 indicating the presence of intact RNA. Total RNA was reverse-transcribed into cDNA as per the manufacturer's instructions using the QuantiTect Reverse Transcription Kit (Qiagen; 15 min, 42 °C; 5 min, 95 °C) or using GoScript™ Reverse Transcription System (Promega; 5 min, 70 C; 5 min, 4 °C; 5 min, 25 °C; 60 min, 42 °C, 15 min, 70 °C) using a BioRad T1000 Thermal Cycler. cDNA was diluted four-fold in nuclease-free water and 2 μl diluted cDNA was used per reaction in the qPCR analysis detailed below. Expression of hnRNA or mRNA in samples was calculated based on the Pfaffl method of relative quantification [16] using primer/probes as previously published [14] and standardized to the expression of house-keeping genes (HKGs) hypoxanthine phosphoribosyltransferase 1 (Hprt1) and tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta (Ywhaz). These genes were selected as HKGs due to their stability across experimental groups (Suppl. Fig. 2 (metyrapone experiment) & 3 (ADX experiment)). The data were expressed as fold change relative to the relevant control condition.

Kennedy C.L.M., Carter S.D., Mifsud K.R, & Reul J.M.H.M. (2020). Unexpected effects of metyrapone on corticosteroid receptor interaction with the genome and subsequent gene transcription in the hippocampus of male rats. Journal of neuroendocrinology, 32(2).

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RNA Extraction and RT-qPCR for Aquatic Viruses

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For the cases investigated, an automated tissue homogenization of samples was performed using the MagNA Lyser instrument (Roche, Basel, Switzerland). Total RNA was extracted using a robot (Roche MagNA Pure LC instrument) with the MagNA Pure LC RNA isolation kit III—Tissue (for the virus), according to the manufacturer’s instructions. The extracted RNA was eluted in 50 μL of nuclease-free water, RNA yields were quantified, and purity was analyzed using the OD260/280 ratio and a NanoPhotometer^® P 300 (Implen, Westlake Village, CA, USA). The eluted RNA was tested immediately following quantitation. The RT-qPCR analysis was done using Light-Cycler 480 RNA Master Hydrolysis Probes for RNA (Roche). For the detection of IPNV, thermocycling conditions and specific primers described by Ingerslev et al. [33 (link)] were used. For piscine orthoreovirus (PRV), the RT-qPCR was done using PRV-specific primers and conditions as described by Palacios et al. [34 (link)]. For the detection of infectious salmon anemia virus (ISAV) in tissue homogenates, ISAV-specific primers and conditions described by Snow et al. [35 ] were used.

Godoy M., Kibenge M.J., Montes de Oca M., Pontigo J.P., Coca Y., Caro D., Kusch K., Suarez R., Burbulis I, & Kibenge F.S. (2022). Isolation of a New Infectious Pancreatic Necrosis Virus (IPNV) Variant from Genetically Resistant Farmed Atlantic Salmon (Salmo salar) during 2021–2022. Pathogens, 11(11), 1368.

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Genomic DNA Extraction from Whole Blood

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From whole-blood samples collected from each subject in 2 and 3 mL sterile vacutainers with anticoagulant ethylenediaminetetraacetic acid (EDTA), DNA was extracted using a Ready DNA Spin Kit (inno-train Diagnostik GmbH, Kronberg, Germany) with silica spin filter technology. In total, 200 µL of whole blood was mixed with 200 µL of lysis buffer and 25 µL of proteinase K and incubated at 56 °C for 10 min, and then 200 µL of absolute ethanol was added. The resulting mixture was transferred to a spinning column and centrifuged for 1 min at 10,000× g.
To purify the DNA, consecutive DNA washes with buffer 1 (kit reagent reconstituted with absolute ethanol) and buffer 2 (kit reagent reconstituted with absolute ethanol) were performed in sterile tubes, followed by 1 min centrifugation at 10,000× g. After the last wash and the subsequent third centrifugation, the DNA was eluted from the spinning column and transferred at room temperature to a sterile tube in TRIS buffer (kit reagent), being subjected to a final 1 min centrifugation at 10,000× g. The DNA was then concentrated, its purity being analyzed using a NanoPhotometer P300 (Implen GmbH, Munich, Germany).

Chiorean A.D., Nicula G.Z., Bâlici Ș., Vică M.L., Iancu Loga L.I., Dican L, & Matei H.V. (2024). HLA Class II Allele Groups Involved in Autoimmune Thyroid Diseases: Hashimoto’s Thyroiditis and Basedow–Graves Disease. Life, 14(4), 441.

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Plasmid DNA Extraction and Quantification

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Plasmid vectors were created using Invitrogen's pCRTM2.1 TOPOTM cloning vectors per the manufacture's speci cations. Ligated vectors were then transformed into DH5α™ cells per the manufacture's speci cations. Successfully, transformed colonies subsequently grown overnight in broth.
Plasmid DNA was puri ed using a Thermo Scienti c GeneJET Plasmid Miniprep Kit (#K0503). To determine 16S copy number for each sample we rst calculated the mass of a single plasmid containing our insert using the Applied Biosystems equation. An Implen nanophotometer P300 was used to assess DNA concentration of the puri ed plasmid solution and subsequent 10-fold serial dilutions were made.
The dilutions were then used as standards for all subsequent qPCR quanti cations. Total copy number was determined by rst calculating the 'raw' copy number (n raw ) in 1µl of DNA based on the Cq value and the standard curve using the formula n raw = logarithmic trendline function. To determine the total number of copies present in each extraction the n raw value was normalized according to elution volume and any subsequent dilution(s).

Anderson K.E., Floyd A., Maes P., Mott B.M., & Copeland D. (2022). Microbiomes associated with European foul brood disease, and idiopathic brood disease syndrome in honey bees. .

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DNA Extraction from Wastewater and Dam Water

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WWTP WW and dam-water samples were filtered through membrane filters (20 mL of WW from the nitrification tank, 200 mL of WW from the sedimentation tank, and 200 mL of water from the dam). The volume of filtered samples from the sedimentation tank and the dam was increased due to an insufficient amount of DNA. The filters were preserved at −80 °C. To extract DNA from the filters, a suspension of 1 mol/L CaCO₃ was added to the filters and incubated overnight at 4 °C. Subsequently, bacterial DNA was isolated according to a previously described method (method number 4) [28 (link)]. The purity and concentration of the isolated DNA were analyzed using the Nanophotometer P300 (IMPLEN, Munich, Germany). DNA samples were frozen at −20 °C.

Stachurová T., Sýkorová N., Semerád J, & Malachová K. (2022). Resistant Genes and Multidrug-Resistant Bacteria in Wastewater: A Study of Their Transfer to the Water Reservoir in the Czech Republic. Life, 12(2), 147.

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Total RNA Extraction from Aurantiochytrium

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The total RNA of Aurantiochytrium sp. PKU#SW7 was extracted with RNAEx^TM total RNA isolation solution (Generay, Shanghai, China) according to the manufacturer’s guidelines. Briefly, samples were homogenized in 1 mL RNAEx^TM using a MP homogenizer (MP FastPrep^®-24, Santa Ana, CA, USA). Then, 200 μL of chloroform was added and the mixtures were incubated for 2 min at room temperature. After centrifugation at 12,000 g for 10 min, the aqueous phase was transferred to a fresh tube, and 500 μL of isopropyl alcohol was added for RNA precipitation and recovery through centrifugation at 12,000 g for 10 min. Afterwards, 1 mL of 75% ethanol was added for RNA washing and the RNA pellet was dissolved in DEPC (diethyl pyrocarbonate) treated water. The integrity of RNA was detected by agarose gel electrophoresis and the concentration was estimated by a UV-Vis spectrophotometer Nanophotometer P300 (Implen, Munich, Germany) and Agilent 2100 bioanalyzer (Agilent, Santa Clara, CA, USA). RNA samples with OD260/280 ≥ 1.8, OD260/230 ≥ 1.8, and RIN > 9.5 were selected for cDNA library construction. Finally, the cDNA libraries were sequenced at Beijing Genomic Institute (BGI)-Shenzhen, China, using Illumina Hiseq^TM 2000 according to the manufacturer’s instructions (Illumina, San Diego, CA, USA).

Liang Y., Liu Y., Tang J., Ma J., Cheng J.J, & Daroch M. (2018). Transcriptomic Profiling and Gene Disruption Revealed that Two Genes Related to PUFAs/DHA Biosynthesis May be Essential for Cell Growth of Aurantiochytrium sp.. Marine Drugs, 16(9), 310.

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