Sample collection. A total of 6 OSF tissue samples and 6 normal buccal mucosa tissues (Nor) were obtained from the Xiangya Hospital of Central South university according to the legislation and the Ethics Board of Xiangya Hospital. All subjects or their caregivers provided written informed consent. All samples were collected and identified by histopathological evaluation. All the samples were stored at -80˚C until being used.
Cell culture and treatment. HaCaT cells were purchased from ProCell Co. (Wuhan, China). All the cells were cultured in MEM supplemented with 15% fetal bovine serum in a 5% CO 2 humidified atmosphere at 37˚C. The cells were transfected with miR-203 mimics, miR-203 inhibitor, and a negative control (NC) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) at a final concentration of 30 nM. To detect the effect of arecoline on EMT, the HaCaT cells were treated with arecoline (Selleck Chemicals, Houston, TX, uSA) at the indicated concentrations for 72 h.
Cell culture and treatment. HaCaT cells were purchased from ProCell Co. (Wuhan, China). All the cells were cultured in MEM supplemented with 15% fetal bovine serum in a 5% CO 2 humidified atmosphere at 37˚C. The cells were transfected with miR-203 mimics, miR-203 inhibitor, and a negative control (NC) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) at a final concentration of 30 nM. To detect the effect of arecoline on EMT, the HaCaT cells were treated with arecoline (Selleck Chemicals, Houston, TX, uSA) at the indicated concentrations for 72 h.