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Ethilon 9 0

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Ethilon® 9-0 is a sterile, non-absorbable, monofilament surgical suture. It is composed of a long-chain aliphatic polyamide polymer, nylon.

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Lab products found in correlation

Vicryl 3, by Johnson & Johnson (1 mentions) Alfamine, by Alfasan (1 mentions) Alfazyne, by Alfasan (1 mentions) Lactated ringers, by Pfizer (1 mentions) 8 0 prolene, by Johnson & Johnson (1 mentions)

7 protocols using ethilon 9 0

1

Sciatic Nerve Transection and Repair in Rats

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The rats were subjected to general anesthesia using an intraperitoneal injection of a combination of ketamine (75 mg/kg) and xylazine (10 g/kg). Following that, the rats were carefully placed in a supine position on the surgical table. The sciatic nerves’ right and left sciatic nerve exposure was performed using an aseptic technique, commencing 1 cm distal to the sciatic notch and continuing 1 cm distal to the nerve’s trifurcation. The nerve segments, approximately 3–3.5 cm long and proximal to the trifurcation, were carefully dissected to isolate the sciatic nerve from the surrounding soft tissue. Following that, the nerves were surgically transected using micro scissors at a precise location located 1.5 cm proximal to the trifurcation site. This indicates the point of origin of the tibial nerve, common peroneal nerve, and caudal sural cutaneous nerve. The surgeon employed three epineural sutures (Ethilon® 9-0, Ethicon, Raritan, NJ, USA) to reestablish the nerves’ connection. Following the procedure, the incision was closed using a 3-0 Vicryl® material. Subsequently, the rats were provided with a period of recovery. After the rats regained consciousness from anesthesia, they were returned to their enclosures and provided unrestricted food and water resources.
Yalçın M.B., Bora E.S, & Erbaş O. (2024). The Effect of Liraglutide on Axon Regeneration and Functional Recovery after Peripheral Nerve Lesion. Current Issues in Molecular Biology, 46(1), 327-339.
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2

Rat Sciatic Nerve Transection and Repair

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Under the induction of general anesthesia of 75 mg/kg ketamine (Alfamine; Alfasan International B.V. Holland) and 10 g/kg xylazine (Alfazyne; Alfasan International B.V. Holland) administered by intraperitoneal injection, rats were fixed to the operating table in the prone position. Both sciatic nerves were exposed from 1 cm distal of the sciatic notch to 1 cm distal to trifurcation of the nerve using an aseptic technique. 3–3.5 cm long nerve segments above the trifurcation were dissected carefully to isolate the sciatic nerve from surrounding soft tissue. The nerves were then transected by the micro scissors at a level of 1.5 cm above the trifurcation (i.e., starting point of the tibial nerve, common peroneal nerve, and caudal sural cutaneous nerve). Nerves were repaired with three epineural sutures (Ethilon® 9-0; Ethicon, Somerville, NJ, USA) by the same surgeon. The wound was closed with a Vicryl® 3-0 (Ethicon), and the rats were allowed to recover. After the recovery from anesthesia, rats were put back to their cages and allowed freely to get food and water.
Gurkan G., Erdogan M.A., Yigitturk G, & Erbas O. (2021). The Restorative Effect of Gallic Acid on the Experimental Sciatic Nerve Damage Model. Journal of Korean Neurosurgical Society, 64(6), 873-881.
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3

Sciatic Nerve Transection and Repair in Rats

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Under the induction of general anesthesia (75 mg/kg ketamin and 10 g/kg xylazine, administered by intraperitoneal injection), rats were fi xed to the operating table in prone position. Both sciatic nerves were exposed from 1 cm distal of the sciatic notch to 1 cm distal to trifurcation of the nerve using aseptic technique. 3-3.5 cm long nerve segments above the trifurcation were dissected carefully to isolate the sciatic nerve from surrounding soft tissue. Then the nerves were transected by the micro scissors at a level of 1.5 cm above the trifurcation (i.e., starting point of tibial nerve, common peroneal nerve, and caudal sural cutaneous nerve).
Nerves were repaired with three epineural sutures (Ethilon® 9-0, Ethicon) by the same surgeon.
The wound was closed with a 3-0 Vicryl® and the rats were allowed to recover. After the recovery from anesthesia, rats were returned to their cages and allowed freely to get food and water.
Karahan G., Kaya H., Erdogan M.A., Yigitturk G., Gokyayla E, & Erbas O. (2019). Effects of trimetazidine on nerve regeneration in a rat sciatic nerve injury model. Bratislavske lekarske listy, 120(10).
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4

Spinal Cord Stimulation for Limb Activation

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Stimulation was delivered via stainless steel wires (AS632, Cooner Wire, Chatsworth, CA, USA) implanted on the midline of the spinal cord facing the dorsal aspect over segments L2 and S1 23 . The wires were secured with sutures (Ethilon 9.0, Ethicon, Johnson & Johnson Medical Ltd, New Brunswick, NJ, USA) either side of the notch to the spinal dura. The implanted wires were subcutaneously tunneled to a skull mounted head plug (MCS-16SS; Omnetics, Minneapolis, MN, USA), and stimulated via a stimulation (S88X stimulator; Astro-Med®, Inc. Grass instruments, Middleton, WA, USA) and isolation unit (SIU-V Isolation unite; Astro-Med®, Inc. Grass instruments, Middleton, WA, USA). Animals were suspended in a custom-made jacket during training. Stimulation was delivered using continuous rectangular pulses (200 s in duration at 40 Hz, with no delay) 20, 23, 37 with a voltage sufficient to activate the hind limb muscles.
Kissane R.W.P., Wright O., Al'Joboori Y.D., Marczak P., Ichiyama R.M, & Egginton S. (2019). Effects of treadmill training on microvascular remodeling in the rat after spinal cord injury. Muscle & nerve, 59(3).
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5

Sciatic Nerve Allograft Repair in Rats

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Female Sprague-Dawley rats were anesthetized with inhaled isoflourane and the left hindlimb shaved with clippers and prepped aseptically. A 1-cm incision was made parallel, and just caudal, to the femur. Using sharp dissection, the cephalad border of the biceps femoris was freed to allow for caudal retraction and exposure of the left sciatic nerve. The exposed nerve was then dissected free of perineural tissue using sharp dissection and minimal retraction.
A 5 mm segment of sciatic nerve was then excised with iris scissors and the wound was irrigated with Lactated Ringers (Hospira; Lake Forest, IL). Using standard microsurgical techniques, the processed allograft was trimmed at each end, for a final graft length of 10mm, and was then sutured in place using 9-0 Ethilon (Ethicon, Sommerville, NJ). The trimmed ends of the chondroitinase ABC treated grafts were collected and frozen for C4S analysis. Proximal and distal ends were approximated in an end-to-end fashion using an interrupted suturing technique and microscopic magnification, followed by wound irrigation with sterile Lactated Ringers. All animals were then given a subcutaneous injection of ketoprofen (5 mg/kg) and allowed to emerge from anesthesia.
Boyer R.B., Sexton K.W., Rodriguez-Feo C.L., Nookala R., Pollins A.C., Cardwell N.L., Tisdale K.Y., Nanney L.B., Shack R.B, & Thayer W.P. (2014). Adjuvant neurotrophic factors in peripheral nerve repair with chondroitin sulfate proteoglycan-reduced acellular nerve allografts. The Journal of surgical research, 193(2), 969-977.
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6

Sciatic Nerve Autograft Reversal with LIPUS

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80 SD rats were randomly divided into four groups: the control group (n = 20): autografting without ultrasonic treatment, low-dose group (n = 20): autografting with low-dose LIPUS treatment, mid-dose group (n = 20): autografting with mid-dose LIPUS treatment and high-dose group (n = 20): autografting with high-dose LIPUS treatment. Rats were anesthetized by 40 mg.kg−1 injection of 2% sodium pentobarbital into abdominal cavity.
All the rats received sciatic nerve autograft reversal transplantation surgery on the right hind legs. A 10-mm-long sciatic nerve was excised and then grafted in the reverse direction between the two nerve stumps using 9-0 Ethilon (Ethicon Inc., Sommerville, NJ, USA) sutures on either side to manufacture a 10-mm-long sciatic nerve autograft models. Muscle and skin were closed with 3-0 Ethilon sutures. All animals were raised in cages after operation.
5 rats of each group were sacrificed at week 2, 4, 8 and 12 after surgery for electrophysiological evaluation, ultrasonic testing, molecular biological detection and pathology detection.
Jiang W., Wang Y., Tang J., Peng J., Wang Y., Guo Q., Guo Z., Li P., Xiao B, & Zhang J. (2016). Low-intensity pulsed ultrasound treatment improved the rate of autograft peripheral nerve regeneration in rat. Scientific Reports, 6, 22773.
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7

Surgical Induction of Myocardial Infarction in Mice

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Myocardial infarction was induced by ligation of the left anterior descending (LAD) coronary artery in P1 mice as described by Haubner et al. (2012) (link) and Porrello et al. (2013) (link). Briefly, anaesthesia was induced by a combination of hypothermia and breathable (isoflurane) anaesthetic. The animal was secured onto an ice pack for the duration of the procedure to maintain adequate anaesthesia. A 5 mm skin incision was placed over the left thorax above the fifth rib, the skin was blunt dissected and a small opening was created at the fifth intercostal space to visualise the left pulmonary lobes and heart. The LAD coronary artery was identified and ligated (9-0 Ethilon, Ethicon, Somerville, New Jersey, USA). The ribs, pectoral muscles and skin were closed (8-0 Prolene, Ethicon). Analgesia was administrated with one drop of 1/50 bupivacaine (Marcain Polyamp Steripack 0.25%, AstraZeneca, Cambridge, UK) in 0.9% saline (B Braun, Melsungen, Germany) onto the wound. The entire litter was tattooed for identification purposes, dabbed with material from the maternal cage and returned to the nest. Studies were conducted at day 1 and day 21 post-MI to respectively assess successful induction of injury and regeneration with ultrasound, histologic and biochemical techniques.
Castellan R.F., Thomson A., Moran C.M, & Gray G.A. (2020). Electrocardiogram-gated Kilohertz Visualisation (EKV) Ultrasound Allows Assessment of Neonatal Cardiac Structural and Functional Maturation and Longitudinal Evaluation of Regeneration After Injury. Ultrasound in Medicine & Biology, 46(1), 167-179.
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