Equal volumes of protein extracts prepared from equivalent number of cells were loaded onto SDS-PAGE gels to analyze CFTR expression and processing. Gels were then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Burlington, MA, USA). The primary antibody was the anti-CFTR monoclonal antibody 596 (CFF) (against amino acids 1204-1211) at 1:3000 dilution and the secondary antibody was horseradish peroxidase-labeled anti-mouse IgG at 1:3000 (BioRad, Hercules, CA, USA) [39 (link)]. Alpha tubulin was used as loading control and detected by anti-alpha tubulin antibody (1:10,000) (Sigma, Darmstadt, Germany). Images were acquired using ChemiDoc XRS+ imaging system BioRad and analyzed with the Image Lab 6.0.1 software.
Horseradish peroxidase labeled anti mouse igg
Manufactured by Bio-Rad
Sourced in United States
Horseradish peroxidase-labeled anti-mouse IgG is a secondary antibody conjugated with the enzyme horseradish peroxidase. It is used to detect and quantify the presence of mouse immunoglobulin G (IgG) in various immunoassays and other applications.
Automatically generated - may contain errors
2 protocols using horseradish peroxidase labeled anti mouse igg
1
Western Blot Analysis of CFTR Protein
2
Immunoblotting of T Cell Lysates
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