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Mp7452

Manufactured by Vector Laboratories
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The MP7452 is a high-precision spectrophotometric microplate reader capable of absorbance, fluorescence, and luminescence detection. It features a wide wavelength range, adjustable bandwidths, and advanced detector technology to provide accurate and reproducible measurement of a variety of assays performed in microplate format.

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Lab products found in correlation

Mp 7451, by Vector Laboratories (3 mentions) Glp 1 sc 57166, by Santa Cruz Biotechnology (2 mentions) Bcl 2 sc 7382, by Santa Cruz Biotechnology (2 mentions) Bcl xl sc 56021, by Santa Cruz Biotechnology (2 mentions) Unconjugated hla allele specific anti hla a2 monoclonal igg antibody, by Thermo Fisher Scientific (2 mentions) Ab17147, by Abcam (1 mentions) Ab16669, by Abcam (1 mentions) Dab substrate, by Vector Laboratories (1 mentions)

3 protocols using mp7452

1

Exosome Isolation and Characterization

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MHC specific antibody was covalently conjugated to N-hydroxysuccinamide magnetic beads (Pierce) per manufacturer’s protocol. 50 to 100 ug protein equivalent of EVs were incubated with antibody beads overnight at 4°C. The bead bound EV fractions were separated per manufacturer’s protocol. EVs bound to beads were eluted using tris glycine and utilized for downstream analysis. Unconjugated HLA allele-specific anti-HLA A2 monoclonal IgG antibody (Catalogue # 0791HA) was purchased from One Lambda (West Hills, CA, USA), for donor HLA class I specific exosome isolation from recipient mouse plasma total pool of exosomes. Antibodies to insulin (15848–1-AP; used at a dilution of 1:200), TSG 101(28283–1-AP; used at a dilution of 1:500) were purchased from Proteintech Lab; antibodies to GLP1R (sc-390774; used at a dilution of 1:200), GLP-1(sc-57166; used at a dilution of 1:200), Bcl-2 (sc-7382; used at a dilution of 1:200), and Bcl-XL (sc-56021; used at a dilution of 1:200) were purchased from Santa Cruz Biotechnologies, Inc. Secondary antibodies conjugated to HRP (ready-to-use anti-rabbit, anti-mouse were purchased from Vector Lab: MP7451 and MP7452, respectively).
Yu M., Agarwal D., Korutla L., May C.L., Wang W., Griffith N.N., Hering B.J., Kaestner K.H., Velazquez O.C., Markmann J.F., Vallabhajosyula P., Liu C, & Naji A. (2020). Islet Transplantation in the Subcutaneous Space Achieves Long-term Euglycemia in Preclinical Models of Type 1 Diabetes. Nature metabolism, 2(10), 1013-1020.
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2

Immunohistochemical Analysis of ELOVL2, CD3, and CD8

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Serial FFPE sections (4 um) were deparaffinized in xylene and then rehy- drated in 100%, 90% and 70% alcohol successively. Antigen unmasking was performed with a preheated epitope retrieval solution (100X citrate buffer, pH 6.0), and endogenous peroxidase was inactivated by incubation in 3% H2O2 for 20 mins. Next, the sections were preincubated with 10% normal goat serum and then incubated overnight with the following primary antibodies: anti-ELOVL2 antibody (1:50, 20308-1-AP, Proteintech); anti-CD3 antibody (1:100, ab16669, Abcam); anti-CD8 antibody (1:100, ab17147, Abcam). Next, sections were incubated with the corresponding horseradish peroxidase (HRP)-conjugated secondary antibodies (ready-to-use, MP-7451 and MP-7452, VectorLab) for 30 mins at room temperature and and development with DAB substrate (Vector Laboratories). Sections were counterstained with hematoxylin. Slides were scanned using PANNORAMIC Digital Slide Scanners, and QuPath software was used to quantify positive staining cells.
Wang Z., Tao P., Fan P., Wang J., Rong T., Hou Y., Zhou Y., Lu W., Hong L., Ma L., Zhang Y, & Tong H. (2023). Insight of a lipid metabolism prognostic model to identify immune landscape and potential target for retroperitoneal liposarcoma. Frontiers in Immunology, 14, 1209396.
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3

Exosome Isolation and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
MHC specific antibody was covalently conjugated to N-hydroxysuccinamide magnetic beads (Pierce) per manufacturer’s protocol. 50 to 100 ug protein equivalent of EVs were incubated with antibody beads overnight at 4°C. The bead bound EV fractions were separated per manufacturer’s protocol. EVs bound to beads were eluted using tris glycine and utilized for downstream analysis. Unconjugated HLA allele-specific anti-HLA A2 monoclonal IgG antibody (Catalogue # 0791HA) was purchased from One Lambda (West Hills, CA, USA), for donor HLA class I specific exosome isolation from recipient mouse plasma total pool of exosomes. Antibodies to insulin (15848–1-AP; used at a dilution of 1:200), TSG 101(28283–1-AP; used at a dilution of 1:500) were purchased from Proteintech Lab; antibodies to GLP1R (sc-390774; used at a dilution of 1:200), GLP-1(sc-57166; used at a dilution of 1:200), Bcl-2 (sc-7382; used at a dilution of 1:200), and Bcl-XL (sc-56021; used at a dilution of 1:200) were purchased from Santa Cruz Biotechnologies, Inc. Secondary antibodies conjugated to HRP (ready-to-use anti-rabbit, anti-mouse were purchased from Vector Lab: MP7451 and MP7452, respectively).
Yu M., Agarwal D., Korutla L., May C.L., Wang W., Griffith N.N., Hering B.J., Kaestner K.H., Velazquez O.C., Markmann J.F., Vallabhajosyula P., Liu C, & Naji A. (2020). Islet Transplantation in the Subcutaneous Space Achieves Long-term Euglycemia in Preclinical Models of Type 1 Diabetes. Nature metabolism, 2(10), 1013-1020.
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