Mouse anti gfap

Briefly, 6-μm-thick sections from the hippoampal DG of the Foxg1 genotype mice (n = 6 in each group) were prepared for immunofluorescence analyses as described previously [12 (link),13 (link)]. To visualize protein expression, sections were first incubated with primary antibodies, including rabbit anti-GFAP (1:200, Wanleibio, cat # WL0836, Shenyang, China), mouse anti-GFAP (1:200, Proteintech, cat# 60190-1-lg, Rosemont, USA), EGFP (1:200, Beyotime, cat # AG281, Shanghai, China), brain lipid binding protein (BLBP) (1:200, Proteintech, cat # 51010-1-AP, Rosemont, USA), Proliferating Cell Nuclear Antigen (PCNA) (1:250, Proteintech, cat # 10205-2-AP, Rosemont, USA), Tbr2 (1:200, Bioss, cat # bs-11331R, Beijing, China), Oligo2 (1:200, Bioss, cat # bs-11194R, Beijing, China), NeuN (1:1200, Bioss, cat # bs-10394R, Beijing, China) and Dcx (1:200, Proteintech, cat # 13925-1-AP, Rosemont, USA). Immunofluorescence secondary antibodies, Dylight 488 (cat # BA1126) and CY3 (cat # BA1032), were obtained from Boster (Wuhan, China). DAPI (KeyGen Biotech, cat # KGA215-50, Shanghai, China) was used to stain the nuclei. After being severally rinsed in PBT, the sections were mounted using antifade mounting medium (Beyotime, Shanghai, China) and fluorescence was visualized using a fluorescence microscope (BX41, Olympus, Tokyo, Japan).

IF assay for tissue samples was performed according to a previous protocol (Gao and Chen, 2013 (link)). The samples were incubated with rabbit anti-BAF45D (1:100, Proteintech, Chicago, IL, USA), mouse anti-GFAP (1:100, Proteintech, Chicago, IL, USA), mouse anti-NEUN (1:100, Millipore, Belecula, CA, USA) and mouse anti-beta-III-tubulin (1:200, Millipore, Belecula, CA, USA) overnight at 4°C. After washed with PBS, the samples were incubated with Alexa Flour-488 anti-mouse (1:500) and Alexa Fluor-594 anti-rabbit (1:500) antibodies. The nuclei were counterstained with 4, 6-diamidino-2-phenylindole (DAPI). A NIKON Eclipse 80i fluorescence microscope and a NIKON Eclipse Ti-S inverted fluorescence microscope were used for visualization. In some cases, a Leica DMI6000CS confocal microscope was used for the visualization. More descriptions of the antibodies used for IF were shown in Table S1.