Eight-week-old, specific-pathogen free (SPF), male ICR mice were acquired from Beijing HFK Bioscience Co., LTD (Beijing, China). All mice were bred in the Life Sciences animal facility of China Agricultural University in a temperature-controlled (20°C) facility with a 12-h light/dark cycle and received a standard chow diet containing 18% protein, 4% fat, and 5% fiber ad libitum with free access to clean water. The feed and water were changed every morning to keep them fresh. Each group of mice was bred in separate cages. All animal studies were approved by the Ethical Committee of the College of Animal Science and Technology of China Agricultural University, and all subjects gave written informed consent in accordance with the Declaration of Helsinki.
Icr mice
Manufactured by Beijing Huafukang Biotechnology
Sourced in China
ICR mice are a strain of laboratory mice commonly used in biomedical research. They are a general-purpose mouse model with a well-characterized genetic background. ICR mice can be used for a variety of experimental purposes, including the study of disease mechanisms, drug development, and toxicology testing.
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Lab products found in correlation
Sd rats, by Beijing Huafukang Biotechnology (1 mentions)
Balb c mice, by Beijing Huafukang Biotechnology (1 mentions)
Male c57bl 6 mice, by Beijing Huafukang Biotechnology (1 mentions)
Balb c nude mice, by Beijing Huafukang Biotechnology (1 mentions)
Sprague dawley rats, by Beijing Huafukang Biotechnology (1 mentions)
9 protocols using icr mice
1
ICR Mouse Husbandry Protocol
2
Establishment of Glioma-Bearing ICR Mouse Model
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ICR mice (male, 18–20 g) were purchased from Beijing HFK Bioscience Co., Ltd. (Beijing, China). All animals received care in compliance with the guidelines in the Guide for the Care and Use of Laboratory Animals. All procedures were approved by Xuzhou Medical University of China Animal Care and Used committee.
Glioma-bearing ICR mice were prepared by intracranial injection (striatum, 1.8 mm right lateral to the bregma and 3 mm of depth) of 1 × 105 C6-Luci cells suspended in 4 µL of L15 medium into male ICR mice (Li et al., 2014 (link); An et al., 2015 (link)). After 7 days of xenograft glioma, nude mice were randomly divided into five groups (n = 10) and housed in a controlled temperature room with regular alternating cycles of light and darkness.
Glioma-bearing ICR mice were prepared by intracranial injection (striatum, 1.8 mm right lateral to the bregma and 3 mm of depth) of 1 × 105 C6-Luci cells suspended in 4 µL of L15 medium into male ICR mice (Li et al., 2014 (link); An et al., 2015 (link)). After 7 days of xenograft glioma, nude mice were randomly divided into five groups (n = 10) and housed in a controlled temperature room with regular alternating cycles of light and darkness.
3
Comparison of Mouse and Rat Models
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The C57BL/6 male mice (20 to 22 g, 6 to 8 weeks), DBA/1 male mice (21 to 22 g, 8 to 10 weeks), Balb/C mice (21 to 23 g, 6 to 8 weeks), ICR mice (22 to 25 g, 8 to 10 weeks), and SD rats (180-200 g, 6 weeks) were all purchased from the Beijing HFK Bioscience Co. Ltd. (Beijing, China). Animals were housed five per cage at room temperature of 22° ± 2°C in specific pathogen–free conditions under a 12-hour light/12-hour dark cycle, with food and water provided ad libitum. All animal experimental procedures used in this study were approved by the Animal Care and Use Committee of Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College (approval no. 00005958). The animal study also accorded with the Animal Research: Reporting of In Vivo Experiments (ARRIVE) guidelines.
4
Establishing Xenograft Tumor Model in Nude Mice
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Balb/C nude mice (4-5 weeks) and ICR mice (5-6 weeks) were purchased from the Beijing HFK Bioscience Co., Ltd. All animals received care in compliance with the guidelines outlined in the Guide for the Care and Use of Laboratory Animals. The procedures were approved by the Hefei University of Technology Animal Care and Use Committee. To establish a BxPC-3 tumor model, Balb/C nude mice (4~5 weeks) were injected with 5×106 cells with 20% BD gel into the right side of the back. After the tumor volume reached 100 mm3, the mice were used for subsequent experiments.
5
ICR Mouse Behavioral Study
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Fifty male ICR mice (20 ± 1 g) were obtained from Beijing HFK Bioscience Co., Ltd, (Beijing, China). All experiment processes were approved by Jiangsu Normal University Committees (approval No. 3/5/2018) and performed according to the relevant guidelines.
6
Mouse Housing and Care Protocol
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Adult male ICR mice between the ages of 8 to 10 weeks were purchased from Beijing HFK Bioscience Co., Ltd. After admission to the Animal Experimental Center of Hebei Medical University, mice were permitted a recovery period of at least seven days in isolation room. After confirming that the mice were healthy, they will be transferred to the feeding room to wait for the experiment. All animals were housed randomly in groups of four in IVC mouse cages under SPF housing conditions with food and water ad libitum. The ambient temperature was set at 20–22°C, humidity at 50–60%, dark/light cycle at 12 h/12 h (lights on at 20:00 p.m. and off at 08:00 a.m. All experiments were approved by the Local Committee on Animal Care and Use and Protection of Hebei Medical University and performed in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals guidelines.
7
Mouse Husbandry for Biomedical Research
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Six-week-old male ICR mice were purchased from Beijing Huafukang Biotechnology Co., Ltd, Beijing, China. The mean animal weight was 22 ± 2 g. The animals were raised in a specific pathogen-free environment at room temperature (22°C) and relative humidity of 50%, with lights on between 06:00 to 18:00 hours. The mice were provided food and water ad libitum; each cage (25 × 15 × 18 cm3) housed five mice. The study received ethical approval from the experimental animal center of the Cancer Hospital of the Chinese Academy of Medical Sciences (the ethical approval code: XYXKJing2019-0019).
8
Animal Husbandry for Research Protocols
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Institute of Cancer Research (ICR) mice (male, 3 weeks, 18–22 g) and Sprague Dawley (SD) rats (male and female, 3 weeks) at Specific Pathogen Free (SPF) grade were provided by Beijing HFK Bioscience Co., Ltd. (Beijing, China). All animals were housed in plastic animal cages in a ventilated room. The room was maintained at 20–26 °C and 40–60% relative humidity with a 12-h light/dark cycle. Water and commercial laboratory complete food were available ad libitum. They were acclimated to the environment for 7 days before the experiment.
9
ICR Mice Surgical Protocol
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Equal numbers of adult male and female ICR mice (n = 48; 8 weeks of age) weighing 20-22 g were obtained from the Beijing HFK Bioscience Co., Ltd., and used in the study. The animals were housed in a polyacrylic cage with four mice per cage and maintained under standard housing conditions (22 °C ± 2 °C and humidity 50 % ± 5 %) with a 12-h light/12-h dark cycle. Food and water were available ad libitum. All efforts were made to minimise the number of animals and their suffering throughout the experiments. The food was withdrawn 16-18 h prior to the surgical procedure. All animal studies were performed in strict accordance with the P. R. China legislation on the use and care of laboratory animals and the guidelines established by the Institute for Experimental Animals at Liaoning University of Traditional Chinese Medicine (131/2010; approved on November 1, 2011).
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