Dimethyl γ butyrolactone acrylate dba glycidyl methacrylate gma acrylic acid 2 2 azobis 2 methylpropionitrile azobisisobutyronitrile aibn n n methylenebisacrylamide mba piperazine pip glycine

NiPAAm, dimethyl-γ-butyrolactone acrylate (DBA), glycidyl methacrylate (GMA), acrylic acid (AA), 2,2’-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N’-methylenebisacrylamide (MBA), piperazine (PiP), glycine, and glutaraldehyde were purchased from Sigma Aldrich (Sigma, St. Louis, MO) and used as received. Anhydrous 1,4-dioxane, diethyl ether, and acetone in analytical grade; and water, acetonitrile, chloroform, and methanol in HPLC-grade were purchased from VWR (Radnor, PA) and used as received. PBS (powder, pH 7.4) was obtained from Gibco Life, Grand Island, NY. Ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA). Acidic gelatin (IEP=5.0) was obtained from Nitta Gelatin (Osaka, Japan). Complete osteogenic medium (COM) was made from minimal essential medium α modification (αMEM) (Gibco Life, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Cambrex BioScience, Walkersville, MD), 10⁻⁸ M dexamethasone, 10 mM β-glycerol 2-phosphate, 50 mg/L ascorbic acid, and 10 mL/L antibiotic-antimycotic solution (Gibco, Life, Grand Island, NY). Live/Dead viability/cytotoxicity kit was purchased from Molecular Probes (Eugene, OR). The calcium assay was purchased from Genzyme Diagnostics (Cambridge, MA).

NiPAAm, dimethyl-γ-butyrolactone acrylate (DBA), glycidyl methacrylate (GMA), acrylic acid (AA), 2,2’-azobis(2-methylpropionitrile) (azobisisobutyronitrile, AIBN), N,N’-methylenebisacrylamide (MBA), piperazine (PiP), glycine, and glutaraldehyde were purchased from Sigma Aldrich (Sigma, St. Louis, MO) and used as received. Anhydrous 1,4-dioxane, diethyl ether, and acetone in analytical grade; and water, acetonitrile, chloroform, and methanol in HPLC-grade were purchased from VWR (Radnor, PA) and used as received. Phosphate buffered saline (PBS) (powder, pH 7.4) was obtained from Gibco Life, Grand Island, NY. Ultrapure water was obtained from a Millipore Super-Q water system (Millipore, Billerica, MA). Acidic gelatin (isoelectric point = 5.0) was obtained from Nitta Gelatin (Osaka, Japan). Complete osteogenic medium (COM) was made from minimal essential medium α modification (αMEM) (Gibco Life, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) (Cambrex BioScience, Walkersville, MD), 10⁻⁸ M dexamethasone, 10 mM β-glycerol 2-phosphate, 50 mg/L ascorbic acid, and 10 mL/L antibiotic-antimycotic solution (Gibco, Life, Grand Island, NY). Live/Dead viability/cytotoxicity kit was purchased from Molecular Probes (Eugene, OR). The calcium assay was purchased from Genzyme Diagnostics (Cambridge, MA).