The concentrations of SCFAs including acetate, propionate, butyrate and valerate were analyzed using the gas chromatographic method. Briefly, approximately 1.0 g of feces were first homogenized in the 1.5 mL deionized water. After centrifuged at 15,000× g for 10 min at 4 °C, supernatants (1 mL of each) were acidified with 25% metaphosphoric acid at a ratio of 1:5 (1 volume of acid for 5 volumes of sample) for 30 min on ice. The sample was injected into a GC 2010 series gas chromatograph (Shimadzu, Japan) equipped with a CP-Wax 52 CB column 30.0 m × 0.53 mm i.d (Chrompack, Netherlands). The injector and detector temperatures were 75 °C and 280 °C, respectively. All procedures in GC were performed in triplicate.
Gc 2010 series gas chromatograph
Manufactured by Shimadzu
Sourced in Japan
The GC 2010 series gas chromatograph is a laboratory instrument manufactured by Shimadzu. It is designed to separate and analyze the components of a complex mixture of gases or volatile liquids.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using gc 2010 series gas chromatograph
1
Quantification of Short-Chain Fatty Acids
2
Quantifying Fecal Short-Chain Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SCFAs concentrations of feces were analyzed through a gas chromatographic method previously described by Bosch et al. (2008) (link). Briefly, approximately 1.5 g of feces were first homogenized in 1.5 mL of deionized water. The samples were centrifuged at 15,000 ×g at 4°C for 10 min. Supernatants (1 mL each) were then acidified with 25% metaphosphoric acid at a 1:5 ratio (1 volume of acid for 5 volumes of sample) for 30 min on ice. The sample was injected into a GC 2010 series gas chromatograph (Shimadzu, Japan) equipped with a CP-Wax 52 CB column 30.0 m × 0.53 mm i.d (Chrompack, Netherlands). The injector and detector temperatures were 75 and 280°C, respectively. Total SCFAs were determined as the sum of analyzed acetate, propionate, and butyrate. Additionally, three-branched fatty acids, namely, isobutyrate, isovalerate, and valerate were measured. All procedures were performed in triplicate.
3
Quantification of Fecal Short-Chain Fatty Acids
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of SCFA in feces were analyzed using a gas chromatographic method, as described by Bosch et al. (2008) (link). Approximately 1.5 g of feces were homogenized in 1.5 mL of deionized water. The samples were centrifuged at 15,000 × g at 4 °C for 10 min. Supernatants (1 mL each) were then acidified with 25% metaphosphoric acid at a 1:5 ratio (vol:vol) for 30 min on ice. The sample was injected into a GC 2010 series gas chromatograph (Shimadzu, Japan) equipped with a CP-Wax 52 CB column 30.0 m × 0.53 mm i.d (Chrompack, Netherlands). The injector and detector temperatures were 75 and 280 °C, respectively. Total SCFA were determined as the sum of analyzed acetic acid, propionic acid, butyric acid, and 3-branched fatty acids, namely, isobutyric acid, isovaleric acid and valeric acid. All procedures were performed in triplicate.
4
Fecal SCFA Analysis by Gas Chromatography
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of SCFAs in feces was analyzed using a gas chromatographic method, as described by Bosch et al. (14 (link)). Briefly, approximately 1.5 g of feces was first homogenized in 1.5 ml of deionized water. The samples were centrifuged at 15,000 × g at 4°C for 10 min. Supernatants (1 ml each) were then acidified with 25% metaphosphoric acid at a 1:5 ratio (1 volume of acid for 5 volumes of the sample) for 30 min on ice. The sample was injected into a GC 2010 series gas chromatograph (Shimadzu, Kyoto, Japan) equipped with a CP-Wax 52 CB column 30 m × 0.53 mm i.d. (Chrompack, Rotterdam, Netherlands). The injector and detector temperatures were 75 and 280°C, respectively. Total SCFAs were determined as the sum of analyzed acetate, propionate, and butyrate. All procedures were performed in triplicate.
5
Quantification of Volatile Fatty Acids in Digesta
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The concentration of volatile fatty acids (VFAs) including short chain fatty acids (SCFAs) and branched chain fatty acids (BCFAs) in digesta were analyzed using a gas chromatographic method. Briefly, approximately 1.0g of digesta samples were first homogenized in the 1.5ml deionized water. After being centrifuged at 15,000×g at 4°C for 10min, supernatants (1ml of each) were acidified with 25% metaphosphoric acid at a 1:5 ratio (1 volume of acid for 5 volumes of sample) for 30min while on ice. The sample was injected into a GC 2010 series gas chromatograph (Shimadzu, Japan) equipped with a CP-Wax 52 CB column 30.0m×0.53mm i.d (Chrompack, Netherlands). The injector and detector temperatures were 75 and 280°C, respectively. All procedures were performed in triplicate and total VFAs were determined as the sum of analyzed SCFAs (acetate, propionate, butyrate, and valerate) and BCFAs (isobutyrate and isovalerate).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!