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X tremegen 9 dna transfection reagent

Manufactured by Roche
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X-tremegen 9 DNA Transfection Reagent is a laboratory product designed for the efficient delivery of DNA into a variety of mammalian cell lines. The reagent facilitates the uptake and expression of genetic material within the target cells.

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Lab products found in correlation

E2940, by Promega (2 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions) Ht 29, by American Type Culture Collection (2 mentions) Dulbecco modified eagle medium (dmem), by American Type Culture Collection (2 mentions)

Spelling variants of this product

Transfection reagent (49 citations) X-tremeGEN™ transfection reagent (2 citations)

4 protocols using x tremegen 9 dna transfection reagent

1

Dual Luciferase Assay for NF-κB Activation

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HEK293T cells (2.5 × 105 cells ml−1) were used to seed a 96-well plate. They were transfected, in triplicate, the following day, with WT and mutant RIPK3 plasmids along with 100 ng NF-κB promoter-firefly luciferase reporter plasmid and 100 ng of Renilla luciferase reporter plasmid per well, in the presence of X-tremegen 9 DNA Transfection Reagent (Roche). Luciferase activities were assessed 24 h later, in a dual luciferase assay (Promega, E2940). Firefly luciferase activities were normalized against Renilla luciferase activities.
Liu Z., Garcia Reino E.J., Harschnitz O., Guo H., Chan Y.H., Khobrekar N., Hasek M.L., Dobbs K., Rinchai D., Materna M., Matuozzo D., Lee D., Bastard P., Chen J., Lee Y.S., Kim S.K., Zhao S., Amin P., Lorenzo L., Seeleuthner Y., Chevalier R., Mazzola L., Gay C., Stephan J.L., Milisavljevic B., Boucherit S., Rozenberg F., Perez de Diego R., Dix R.D., Marr N., Béziat V., Cobat A., Aubart M., Abel L., Chabrier S., Smith G.A., Notarangelo L.D., Mocarski E.S., Studer L., Casanova J.L, & Zhang S.Y. (2023). Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Science immunology, 8(82), eade2860.
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2

Generating Immortalized Cell Lines from Human Fibroblasts

Cited 1 time
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Primary human fibroblasts were obtained from skin biopsy specimens from controls and P1, and were cultured in DMEM (GIBCO BRL, Invitrogen) supplemented with 10% fetal calf serum (FCS) (GIBCO BRL, Invitrogen). Immortalized SV40-transformed fibroblast cell lines (SV40-F) and Epstein-Barr virus (EBV)-transformed B-cell lines (EBV-B) were generated as previously described (33 (link)). More technical details are provided in Supplementary Materials. HEK293T, HeLa and HT29 cells (ATCC) were maintained in DMEM supplemented with 10% FCS. SV40-F, HEK293T and HeLa cells were transiently transfected with the aid of X-tremegen 9 DNA Transfection Reagent (XTG9-RO, Roche). RIPK3 KO cell lines were transduced with a lentiviral system, with a mock vector (Luci), or with WT and mutant RIPK3 constructs, and were then cultured under puromycin selection.
Liu Z., Garcia Reino E.J., Harschnitz O., Guo H., Chan Y.H., Khobrekar N., Hasek M.L., Dobbs K., Rinchai D., Materna M., Matuozzo D., Lee D., Bastard P., Chen J., Lee Y.S., Kim S.K., Zhao S., Amin P., Lorenzo L., Seeleuthner Y., Chevalier R., Mazzola L., Gay C., Stephan J.L., Milisavljevic B., Boucherit S., Rozenberg F., Perez de Diego R., Dix R.D., Marr N., Béziat V., Cobat A., Aubart M., Abel L., Chabrier S., Smith G.A., Notarangelo L.D., Mocarski E.S., Studer L., Casanova J.L, & Zhang S.Y. (2023). Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Science immunology, 8(82), eade2860.
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3

Dual Luciferase Assay for NF-κB Activation

Check if the same lab product or an alternative is used in the 5 most similar protocols
HEK293T cells (2.5 × 105 cells ml−1) were used to seed a 96-well plate. They were transfected, in triplicate, the following day, with WT and mutant RIPK3 plasmids along with 100 ng NF-κB promoter-firefly luciferase reporter plasmid and 100 ng of Renilla luciferase reporter plasmid per well, in the presence of X-tremegen 9 DNA Transfection Reagent (Roche). Luciferase activities were assessed 24 h later, in a dual luciferase assay (Promega, E2940). Firefly luciferase activities were normalized against Renilla luciferase activities.
Liu Z., Garcia Reino E.J., Harschnitz O., Guo H., Chan Y.H., Khobrekar N., Hasek M.L., Dobbs K., Rinchai D., Materna M., Matuozzo D., Lee D., Bastard P., Chen J., Lee Y.S., Kim S.K., Zhao S., Amin P., Lorenzo L., Seeleuthner Y., Chevalier R., Mazzola L., Gay C., Stephan J.L., Milisavljevic B., Boucherit S., Rozenberg F., Perez de Diego R., Dix R.D., Marr N., Béziat V., Cobat A., Aubart M., Abel L., Chabrier S., Smith G.A., Notarangelo L.D., Mocarski E.S., Studer L., Casanova J.L, & Zhang S.Y. (2023). Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Science immunology, 8(82), eade2860.
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4

Generating Immortalized Cell Lines from Human Fibroblasts

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary human fibroblasts were obtained from skin biopsy specimens from controls and P1, and were cultured in DMEM (GIBCO BRL, Invitrogen) supplemented with 10% fetal calf serum (FCS) (GIBCO BRL, Invitrogen). Immortalized SV40-transformed fibroblast cell lines (SV40-F) and Epstein-Barr virus (EBV)-transformed B-cell lines (EBV-B) were generated as previously described (33 (link)). More technical details are provided in Supplementary Materials. HEK293T, HeLa and HT29 cells (ATCC) were maintained in DMEM supplemented with 10% FCS. SV40-F, HEK293T and HeLa cells were transiently transfected with the aid of X-tremegen 9 DNA Transfection Reagent (XTG9-RO, Roche). RIPK3 KO cell lines were transduced with a lentiviral system, with a mock vector (Luci), or with WT and mutant RIPK3 constructs, and were then cultured under puromycin selection.
Liu Z., Garcia Reino E.J., Harschnitz O., Guo H., Chan Y.H., Khobrekar N., Hasek M.L., Dobbs K., Rinchai D., Materna M., Matuozzo D., Lee D., Bastard P., Chen J., Lee Y.S., Kim S.K., Zhao S., Amin P., Lorenzo L., Seeleuthner Y., Chevalier R., Mazzola L., Gay C., Stephan J.L., Milisavljevic B., Boucherit S., Rozenberg F., Perez de Diego R., Dix R.D., Marr N., Béziat V., Cobat A., Aubart M., Abel L., Chabrier S., Smith G.A., Notarangelo L.D., Mocarski E.S., Studer L., Casanova J.L, & Zhang S.Y. (2023). Encephalitis and poor neuronal death-mediated control of herpes simplex virus in human inherited RIPK3 deficiency. Science immunology, 8(82), eade2860.
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