In vitro drug release studies were performed using an Automated Transdermal Diffusion Cells Sampling System (Logan System 912-6, Somerset, KY, USA). The drug-loaded samples PDMSCN-CoAM, PDMSPA1, CN-CoAM, PDMSPA2,CN-CoAM, PDMSPA3, CN-CoAM, PDMSPA5, CN-CoAM were cut into 10 mm × 10 mm squares and placed on the top of a PET mesh. The receptor compartment was filled with Phosphate Buffered Saline (PBS, purchased from Sigma-Aldrich, Germany) with a pH value of 7.4 and its temperature was maintained at 37 °C. During the dissolution testing the medium was stirred continuously with a magnetic bar. Samples were collected over a period of 24 h at different time intervals (1, 5, 10, 20, 30, 60, 120, 180, 240, 300, 360 and 1440 min), while the released/dissolved CoAM concentration in the receptor medium was determined by a UV-Vis spectrophotometer (Cary 60 UV-Visible Spectrophotometer, Agilent, Germany) by quantification of the absorption band at 276 nm. The withdrawn sample volumes were replaced by fresh PBS with a stable temperature of 37 °C of the same volume. Sink conditions were assured due to sample withdrawal, followed by sample dilution through media replacement. In calculation of concentrations using the Beer–Lambert Law, this dilution was accounted for. All release studies were performed in triplicates and are reported as average value with standard errors.
Cary 60 uv visible spectrophotometer
Manufactured by Agilent Technologies
Sourced in United States, Germany
The Cary 60 UV-visible spectrophotometer is a laboratory instrument used to measure the absorbance or transmittance of light by a sample across the ultraviolet and visible regions of the electromagnetic spectrum. It is designed to quantify the concentration of analytes in a solution by analyzing the interaction of light with the sample.
Automatically generated - may contain errors
Lab products found in correlation
Quartz cuvette, by Hellma (2 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Hematocrocrit centrifuge, by Thermo Fisher Scientific (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Cary winuv software package, by Agilent Technologies (1 mentions)
Avance 3 hd spectrometer, by Bruker (1 mentions)
Model 3k 30, by Merck Group (1 mentions)
Jem 3010 uhr, by JEOL (1 mentions)
Lambda 35 uv visible spectrophotometer, by PerkinElmer (1 mentions)
Jem 2100, by JEOL (1 mentions)
Alpha 300r confocal raman microscope, by WITec (1 mentions)
Uv 2450, by Shimadzu (1 mentions)
Sigma 3 30 ks, by Merck Group (1 mentions)
Vivaspin 6 concentrator, by Sartorius (1 mentions)
Optima 2100, by PerkinElmer (1 mentions)
Nanolab g3 cx, by Thermo Fisher Scientific (1 mentions)
Isoniazid inh, by Merck Group (1 mentions)
Whatman filter paper, by Cytiva (1 mentions)
Whatman, by Cytiva (1 mentions)
K4fe cn 6, by Merck Group (1 mentions)
39 protocols using cary 60 uv visible spectrophotometer
1
In Vitro Drug Release from PDMS-based Films
2
Hemoprotein UV-Vis Spectroscopy Protocol
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The UV-visible spectra of WT and mutant forms OleTJE were collected using a Cary 60 UV-visible spectrophotometer (Agilent). Spectra were recorded at ambient temperature for the proteins in buffer A, typically at concentrations of ∼4–6 μm . The ferrous and FeII-CO forms of the hemoproteins were produced under anaerobic conditions using degassed buffer A. Reduced enzymes were formed by the addition of a few grains of solid sodium dithionite to ferric enzymes. The FeII-CO complexes were then formed by slowly bubbling CO gas into reduced OleTJE proteins until no further spectral shift was observed.
3
Bacterial Pigment Extraction and Quantification
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At the end of the incubation, bacterial growth was quantified at 764 nm (
Joshi
et al., 2016 ). This was followed by pigment extraction and quantification, as per the method described below for each of the pigments. Purity of each of the extracted pigment was confirmed by running a UV-vis scan (Agilent Cary 60 UV-visible spectrophotometer). Appearance of single major peak (at the λ
max reported in literature) was taken as indication of purity.
et al., 2016
max reported in literature) was taken as indication of purity.
4
Photometric Bacterial Growth Quantification
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At the end of the incubation, bacterial growth was quantified photometrically by measuring the culture density at 764 nm wavelength [19 (link)]. This was followed by pigment extraction and quantification, as per the method described below for each of the pigment. Purity of each of the extracted pigment was confirmed by running a UV-vis scan (Agilent Cary 60 UV-visible spectrophotometer). Appearance of single major peak (at the λmax reported in literature) was taken as indication of purity.
5
Quantification of Hemolysis in Blood Samples
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Percent hemolysis was measured based on % hematocrit, supernatant hemoglobin (Hb) (g/dL), and total (Hb) (supernatant + RBC, g/dL) in 50 microliter samples obtained weekly from storage bags. Supernatant and RBCs were separated using a hematocrocrit centrifuge (ThermoFisher, Frederick, MD, United States). Hematocrit was recorded and supernatant was separated from RBCs. Supernatant and lysed RBC Hb levels were measured using a Cary 60 UV-visible spectrophotometer (Agilent Technologies, Santa Clara, CA, United States). Oxy ferrous Hb (HbFe2+O2) and ferric Hb (HbFe3+) concentrations were determined based on the extinction coefficients for each species. Molar extinction coefficients used to calculate Hb concentrations in heme equivalents were: 15.2 mM–1 cm–1 at 576 nm for Hb(O2) and 4.4 mM–1 cm–1 at 631 nm for ferric Hb using 50 mM potassium phosphate buffer, pH 7.0, at ambient temperature, in both cases. Total heme was calculated by adding these values and converting (heme) (microM) to total (Hb) (g/dL).
6
Bacterial Pigment Extraction and Quantification
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At the end of the incubation, bacterial growth was quantified at 764 nm [17 (link)]. This was followed by pigment extraction and quantification, as per the method described below for each of the pigment. Purity of each of the extracted pigment was confirmed by running a UV-Vis scan (Agilent Cary 60 UV-visible spectrophotometer). Appearance of a single major peak (at λmax reported in the literature) was taken as indication of purity.
7
Quantifying Lipid Oxidation via TBARS
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The TBARS tests were carried out as described previously with some modifications [6 (link)]. Briefly, 100 mg of lipid sample was mixed with 25 mL of 1-butanol. Then, 5 mL of the mixture was transferred into a screw-capped bottle containing 5 mL of freshly prepared TBA reagent (0.5 g of TBA in 250 mL of 1-butanol) and thoroughly mixed. Contents were incubated at 95 °C for 2 h, cooled on ice and centrifuged at 6500 rpm for 5 min. The clear-coloured liquid was transferred to a clean cuvette, and absorbance was read at 535 nm in a Cary 60 UV-Visible Spectrophotometer (Agilent Technologies; Santa Clara, CA, USA). The results were calculated using the formula C = (0.415 × A)/w, where A is the absorbance at 532 nm, w is the sample mass (g), and factor 0.415 was obtained from the calibration curve of the MDA standard (1,1,3,3-tetramethoxypropane). The final results of TBARS were expressed as milligram (mg) MDA equivalents per kilogram (kg) of lipid sample.
8
Citrus Wine Color Analysis
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Citrus wine samples were centrifuged and filtered through 0.45-μm filters prior to analysis. The color values of the wine were evaluated using CIELab color space (Pérez-Caballero et al., 2003 ) and were measured with a Cary 60 UV-visible spectrophotometer (Agilent Technologies) at four wavelengths (450, 520, 570, and 630 nm). The values L* (lightness), a* (from red to green), and b* (from blue to yellow) (Pérez-Caballero et al., 2003 ), as well as C* (chroma), H* (hue-angle), and E* (color difference) (Ramos-Escudero et al., 2012 ), were calculated according to the following six formulae
9
Phenolic Content Determination by Folin-Ciocalteu
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The content of phenols was obtained by a colorimetric method described by Velioglu et al. [11 (link)] with some modifications. Briefly, 100 μL of the extract (at 2 mg/mL in 80% methanol) was mixed with 750 μL of the Folin-Ciocalteu reagent (diluted in a 1/10 proportion of Milli-Q water). After 5 min in the dark, 750 μL of sodium bicarbonate (60 g/L) was added to the mixture. The tubes were kept in the dark for 90 min at 30 °C, then the absorbance was obtained at 725 nm using a Cary60 UV-visible spectrophotometer (Agilent, Santa Clara, CA, USA). Gallic acid (10–100 μg, Y = 0.0076X − 0.0182; R2 = 0.9998) was used for the preparation of the standard curve. The content regarding phenolic compounds was then expressed as mg gallic acid per gram of extract. Three experiments were performed in triplicate for each measurement and the values were recorded as the mean ± SD.
10
Enzymatic Activity Measurement for MGST1, GSTA1, and GSTP1
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