Porcine cholesterol esterase

Gallic acid, rat intestinal acetone powder, porcine pancreatic α-amylase, 4-methylumbelliferone, glucose oxidase kits and 3,5-dinitrosalicylic acid p-nitrophenylbutylrate (p-NPB), oleic acid, phosphatidylcholine, glycodeoxycholic acid, taurodeoxycholic acid, taurocholic acid, porcine cholesterol esterase, porcine pancreatic lipase were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Cholesterol test kits were purchased from HUMAN GmbH Co. (Wiesbaden, Germany). Total bile acid kit was purchased from Bio-Quant Co. (San Diego, CA, USA). All other chemical reagents used in this study were of analytical grade.

Porcine pancreatic lipase, 4-methylumbelliferyl oleate (4-MUO), oleic acid, phosphatidylcholine, glycodeoxycholic acid, taurodeoxycholic acid, taurocholic acid, porcine cholesterol esterase, orlistat, and p-nitrophenylbutylrate (p-NPB) were purchased from Sigma (St. Louis, MO, USA). Total bile acid colorimetric assay kit and cholesterol test kit were obtained from GenWay (San Diego, CA, USA) and HUMAN GmbH Co. (Wiesbaden, Germany), respectively. Cholestyramine resin was purchased from RUBIÓ (Barcelona, Spain). Ezetimibe was purchased from Merck, Sharp & Dohme (Kenilworth, NJ, USA). 22-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-23,24-bisnor-5-cholen-3-ol (NBD-cholesterol) and TRIzol reagent were obtained from Invitrogen (Eugene, OR, USA). Cyanidin-3-rutinoside chloride was synthesized from quercetin-3-rutinoside according to a previous study [24 (link)]. All other chemical reagents used in this study were analytical grade.

Folin-Ciocalteu, quercetin, catechin, gallic acid, rat intestinal acetone powder, porcine pancreatic α-amylase, vanillin, 3,5-dinitrosalicylic acid, glucose oxidase kits, p-nitrophenylbutylrate (p-NPB), p-nitrophenylpalmitate (p-NPP), taurodeoxycholic acid, taurocholic acid, porcine cholesterol esterase, porcine pancreatic lipase were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). All others chemicals used were of analytical grade.