Nicotine nic

Manufactured by Merck Group

Sourced in United States

Nicotine (Nic) is a chemical compound found in the tobacco plant. It is a colorless, volatile, and highly toxic liquid. Nicotine is commonly used in research and laboratory settings as a reference standard for analytical and pharmacological studies.

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Nicotine

5 protocols using nicotine nic

Radioactive α-Btx Binding Assay

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[¹²⁵I]-labelled α-Btx was from Perkin Elmer, while unlabelled α-Btx and 1-(5-Chloro-2,4-dimethoxy-phenyl)-3-(5-methyl-isoxazol-3-yl)-urea, known as PNU-120596, were from Tocris Biosciences. All other chemicals, including acetylcholine chloride (ACh) and nicotine (Nic), were from Sigma-Aldrich.

daCosta C.J., Free C.R, & Sine S.M. (2015). Stoichiometry for α-bungarotoxin block of α7 acetylcholine receptors. Nature Communications, 6, 8057.

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Radioactive α-Bungarotoxin Receptor Assay

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[¹²⁵I]-labeled α-bungarotoxin (α-Btx) was from Perkin Elmer, while unlabeled α-bungarotoxin and 1-(5-Chloro-2,4-dimethoxy-phenyl)-3-(5-methyl-isoxazol-3-yl)-urea, known as PNU-120596 (PNU), were from Tocris Biosciences. All other chemicals, including acetylcholine chloride (ACh) and nicotine (Nic), were from Sigma-Aldrich.

daCosta C.J., Free C.R, & Sine S.M. (2015). Stoichiometry for α-bungarotoxin block of α7 acetylcholine receptors. Nature Communications, 6, 8057.

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Neurotransmitter Receptor Binding Assays

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Methanol, hexane, chloroform, ethyl acetate, butanol, acetonitrile, (−)-nicotine (NIC), methyllycaconitine (MLA) citrate salt hydrate, mecamylamine (MEC) hydrochloride, nomifensine, (−)-lobeline hydrochloride, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma Aldrich (St. Louis, MO, USA). Streptomycin (10,000 μg/ml), penicillin (10,000 units/ml), fetal bovine serum (FBS), and Dulbecco’s Modified Eagle Medium (DMEM) were purchased from Life Technologies Corporation (Grand Island, NY, USA). Quercetin was purchased from Chromadex (Irvine, CA, USA). [³H]-epibatidine (S.A. = 30 Ci/mmol), [³H]-cytisine (S.A. = 16 Ci/mmol), [³H]-MLA (S.A. = 60 Ci/mmol), [³H]-GBR12935 (S.A. = 40 Ci/mmol), ⁴⁵CaCl₂ (S.A. = 12.05 mCi/mg), and [³H]-DA (S.A. = 60 Ci/mmol) were purchased from American Radiolabeled Chemicals, Inc. (St. Louis, MO, USA). All other chemicals and materials were purchased from Fisher Scientific (Pittsburgh, PA, USA), unless otherwise stated.

Brown D.P., Rogers D.T., Pomerleau F., Siripurapu K.B., Kulshrestha M., Gerhardt G.A, & Littleton J.M. (2016). Novel multifunctional pharmacology of lobinaline, the major alkaloid from Lobelia cardinalis. Fitoterapia, 111, 109-123.

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Cytotoxicity Evaluation of Drug Compounds

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MAN (purity > 98%) was purchased from SanHerb Bioscience Inc. (Chengdu,
Sichuan, China). Lipopolysaccharide (LPS, from gram-negative bacteria E.
coli 055:B5), nicotine (Nic) and methyl lycaconitine citrate
hydrate (MLA) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Fetal
bovine serum (FBS), RMPI-1640 medium, phosphate buffered saline (PBS),
penicillin-streptomycin together with enhanced chemiluminescence (ECL) detection
kit were supplied by Thermo Scientific (Rockford, IL, USA). Antibodies and BCA
protein quantitative kit were purchased from Cell Signaling Technology (Beverly,
MA, USA) and Keygen Biotech (Nanjing, Jiangsu, China), respectively. Ultra-pure
water was prepared by using a Milli-Q purification system (Millipore, Bedford,
MA, USA).

Yang Z., Yin Q., Olatunji O.J., Li Y., Pan S., Wang D.D, & Zuo J. (2020). Activation of cholinergic anti-inflammatory pathway involved in therapeutic actions of α-mangostin on lipopolysaccharide-induced acute lung injury in rats. International Journal of Immunopathology and Pharmacology, 34, 2058738420954941.

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Nicotine-Induced O-GlcNAcylation in Breast Cancer

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MCF-7, T47D, MDA-MB-435, and MDA-MB-231 cells were obtained from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China) and were used within 6 months from resuscitation. All the cells were cultured in 90 % RPMI-1640 (Gibco, USA) supplemented with 1% penicillin/streptomycin antibiotics (Gibco, USA) and 10% fetal bovine serum (FBS, Gibco, USA). Nicotine (Nic, Sigma, MO, USA) was added to the cells cultured in indicated concentrations. Azaserine (AZA), PugNAc were purchased from Sigma (MO, USA). L01 was purchased from BioBioPha Co., Ltd. (Kunming, China). Lipofectamine 2000 was from Invitrogen (NY, USA). O-GlcNAc enzymatic labeling system were from Invitrogen (CA, USA). Human CEBPB and CHOP cDNA were respectively subcloned into the pcDNA3.1 mammalian expression vector (Invitrogen, NY, USA). GFAT promoter report constructs were made by cloning wild type 2384 bp of human GFAT promoter (WT-Luc) or mutants (MUT-Luc, GATTACTCCAC → GAAAACTCCAC; ATTACACAAG → AAAACACAAG) into luciferase vector pGL3. Primers were shown in Supporting information Table S1.

Zhang N., Zhu T., Yu K., Shi M., Wang X., Wang L., Huang T., Li W., Liu Y, & Zhang J. (2019). Elevation of O-GlcNAc and GFAT expression by nicotine exposure promotes epithelial‐mesenchymal transition and invasion in breast cancer cells. Cell Death & Disease, 10(5), 343.

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