Anti syntenin

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-Syntenin is a laboratory antibody product manufactured by Santa Cruz Biotechnology. It is designed to detect and measure the presence of the Syntenin protein, which is involved in various cellular processes.

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Lab products found in correlation

Anti slug, by Santa Cruz Biotechnology (1 mentions) Magna chip a g, by Merck Group (1 mentions) Anti hdac1, by Merck Group (1 mentions) Heparin, by Merck Group (1 mentions) Anti lamp1, by Santa Cruz Biotechnology (1 mentions) Anti rab9, by Cell Signaling Technology (1 mentions) Anti rab7, by Cell Signaling Technology (1 mentions) Anti α actinin, by Cell Signaling Technology (1 mentions) Anti myc tag, by Santa Cruz Biotechnology (1 mentions) Phalloidin tritc, by Merck Group (1 mentions) Anti cortactin, by Cell Signaling Technology (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Mouse anti n cadherin, by BD (1 mentions) Mouse anti gfp, by BD (1 mentions) Pegfp, by Thermo Fisher Scientific (1 mentions) Mouse anti vimentin, by BD (1 mentions) Mouse anti β actin, by Santa Cruz Biotechnology (1 mentions) Mouse anti flag, by Merck Group (1 mentions) Mouse anti ha, by Fortrea (1 mentions) Mouse anti e cadherin, by BD (1 mentions)

4 protocols using anti syntenin

Western Blot Antibody Validation

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We used the following commercial antibodies for Western blot analyses: anticalnexin (Sc-6465), anti-CD81 (Sc-166029), and anti-Tsg101 (Sc-7964) from Santa Cruz Biotechnology; anti-CD9 (553758) and anti-Flotillin (610820) from BD Biosciences; anti-FLAG (F3165) from Sigma; anti-Histone H2A.Z (2718) from Cell Signaling; antilactate dehydrogenase (AB1222) from Millipore; and antivimentin (20R-VP004) from Fitzgerald. The antisyntenin antibody used in this study was affinity purified further from antisyntenin (Santa Cruz Biotechnology, sc-48742). We used anti-F-actin (abcam, ab205) for coimmunoprecipitation.

Qiu X., Campos Y., van de Vlekkert D., Gomero E., Tanwar A.C., Kalathur R., Weesner J.A., Bongiovanni A., Demmers J, & d’Azzo A. (2022). Distinct functions of dimeric and monomeric scaffold protein Alix in regulating F-actin assembly and loading of exosomal cargo. The Journal of Biological Chemistry, 298(10), 102425.

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Chromatin Immunoprecipitation of Slug, HDAC1, and Syntenin

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CL1–5, CL1–5/shCtl, or CL1–5/shSyntenin-b (1 × 10⁷) cells were analyzed by Magna ChIP™ A/G (Millipore, Billerica, MA) according to the manufacturer's instructions. Briefly, the cells was cross-linked in culture media with 1% formaldehyde and quenched by 0.125 M glycine. After washing with cold PBS, the cells were scraped and soluble chromatin lysates were extracted by sonication and centrifugation, and then mixed with anti-Slug (Santa Cruz), anti-HDAC1 (Millipore), or anti-Syntenin (Santa Cruz) antibodies and protein A/G magnetic beads overnight at 4°C. The complexes were pelleted and washed with indicated buffers.
The DNA/protein solution was eluted with proteinase K containing elution buffer at 65°C for 2 h to reverse the formaldehyde cross-links. The DNA elutes were purified and E-cadherin-specific amplified by PCR using indicated primers (forward, 5′-CGAACCCAGTGGAATCAGAA-′3 and reverse, 5′-GCGGGCTGGAGTCTGAACTG-3′).

Wang L.K., Pan S.H., Chang Y.L., Hung P.F., Kao S.H., Wang W.L., Lin C.W., Yang S.C., Liang C.H., Wu C.T., Hsiao T.H., Hong T.M, & Yang P.C. (2015). MDA-9/Syntenin-Slug transcriptional complex promote epithelial-mesenchymal transition and invasion/metastasis in lung adenocarcinoma. Oncotarget, 7(1), 386-401.

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Antibodies for Heparanase and Syndecan-1 Detection

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Rabbit polyclonal antibodies #1453 was prepared against purified latent 65 kDa heparanase [8 (link)]. Anti-heparanase monoclonal antibody was kindly provided by ImClone Systems (New York, NY). Anti-heparanase-2 antibody (20C5) has been described [15 (link)]. Rat anti-mouse syndecan-1 monoclonal antibody (clone 281-2) was kindly provided by Dr. Ralph D. Sanderson (UAB). This antibody is directed against the ectodomain of mouse syndecan-1 and is suitable for flow cytometry, immune staining and immunoblotting. Anti-vinculin and anti-actin monoclonal antibodies, phalloidin-TRITC, heparin, and methyl-β-cyclodextrin were purchased from Sigma (St. Louis, MO). Anti-α-actinin, anti-cortactin, anti-Rab7 and anti-Rab9 antibodies were from Cell Signaling (Beverly, MA). Anti-syntenin, anti-LAMP1, and anti-Myc Tag antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). Latent 65 kDa heparanase was purified from medium conditioned by HEK-293 cells over expressing heparanase essentially as described [24 (link)].

Shteingauz A., Ilan N, & Vlodavsky I. (2014). Processing of heparanase is mediated by syndecan-1 cytoplasmic domain and involves syntenin and α-actinin. Cellular and molecular life sciences : CMLS, 71(22), 4457-4470.

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Molecular Cloning and Antibody Validation

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Human MDA-9/Syntenin cDNA were obtained from H1299 by RT-PCR. Its full length or deletion constructs were sub-cloned into pACT2, pcDNA3.1-HA, pCMV-tag2 (Clontech, CA, USA), pEGFP (Invitrogen), and pLKO.1-AS2-neo vectors for the production of lentivirus or pET28a vectors for bacterial expression by standard molecular cloning procedure. Flag-tagged Slug was as described previously [10 (link)]. Full length or deletion constructs of Slug were sub-cloned into pAS2–1, pCI-neo, pcDNA3.1-HA, pDsRED, and AS2-neo vectors. pcDNA3-HDAC1-Flag was kindly provided by Dr. Wen-Ming Yang (Institute of Molecular Biology, National Chung Hsing University, Taichung, Taiwan).
The primary antibodies used for immunoblot analysis were anti-Syntenin (mouse monoclonal, S-31 or rabbit polyclonal, H-48, Santa Cruz Biotechnology, CA, USA and rabbit monoclonal, Abcam, MA, USA), goat anti-Slug (Santa Cruz Biotechnology), goat anti-HDAC1 (Santa Cruz Biotechnology) and mouse anti-β-actin (Santa Cruz Biotechnology), mouse anti-Flag (Sigma-Aldrich, MO, USA), mouse anti-HA (Covance, Berkeley, California, USA), mouse anti-E-cadherin (BD Biosciences, Inc., CA, USA), mouse anti-vimentin (BD Biosciences), mouse anti-N-cadherin (BD Biosciences), and mouse anti-GFP (BD Biosciences).

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