Pe cyanine7 anti mouse cd31

bMSCs were isolated and expanded, as described, with minor modification.²¹ The cells were characterized phenotypically using cell surface markers (positive for CD29, Scal‐1, CD44, and CD105 and negative for CD34, CD45, CD11b, and CD31) with flow cytometric analysis (Figure S1A) and multilineage differentiation with chemical induction and staining, including adipogenic differentiation using oil red O staining, osteogenic differentiation using alizarin red staining, and chondrogenic differentiation using Alcian blue and nuclear fast red staining (Figure S1B through S1E). The antibodies for flow cytometry analysis, including fluorescein isothiocyanate (FITC) anti‐mouse CD29 (102205), Peridinin‐Chlorophyll‐Protein anti‐mouse Sca‐1 (108122), FITC anti‐mouse CD44 (103005), Allophycocyanin (APC) anti‐mouse CD105 (120413), Phycoerythrin anti‐mouse CD34 (119308), APC/cyanine7 anti‐mouse CD45 (103115), AF 700 anti‐mouse CD11b (101222), and PE/cyanine7 anti‐mouse CD31 (102418), were from Biolegend. Cell populations were carefully compensated with isotype antibody staining as control. Fluorescence‐positive cells were quantitatively evaluated using LSRFortessa X‐20 (BD Bioscience, CA), and analyzed using software FlowJo_V10.