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Modular a1 a1r confocal laser scanning microscope system

Manufactured by Nikon

The Modular A1+/A1R+ confocal laser scanning microscope system is a high-performance microscope designed for advanced imaging applications. The system utilizes a modular design that allows for customization to meet specific research requirements. It features a range of laser options, advanced detectors, and specialized optics to provide high-resolution, high-sensitivity imaging capabilities.

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2 protocols using modular a1 a1r confocal laser scanning microscope system

1

Fluorescence Microscopy for Protein Localization

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FWA-GFP in the central cell and endosperm was detected using an Olympus BX53 fluorescence microscope equipped with an Olympus DP80 digital camera [57 (link)]. DRE2-GFP in the root was detected using the Nikon’s modular A1+/A1R+ confocal laser scanning microscope system (National Center for Protein Sciences at Peking University, Beijing).
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2

Immunofluorescence Staining of DRE2-HA Transgenic Plant

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Immunofluorescence staining was performed using leaves from 21-day-old DRE2-HA transgenic plant as previously described [59 (link)]. Nuclei preparations were incubated with a mouse anti-HA monoclonal antibody (Sigma, H3663) overnight at room temperature and then incubated with goat anti-mouse Alexa Fluor 488 secondary antibody (Invitrogen, A-11001) for 2 h at 37°C. DNA was counterstained using DAPI in Prolong Gold (Invitrogen, P36931). Pictures were taken using the Nikon’s modular A1+/A1R+ confocal laser scanning microscope system (National Center for Protein Sciences at Peking University, Beijing).
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