Five micron sections of tumor biopsies were subjected to immunohistochemistry using Ventana reagents and a Ventana autostainer in a CLIA histology laboratory. Each biopsy, dependent upon sufficient sample, was assessed for the expression of ER, PR, AR, HER2 and Ki67.All stained sections, including H&Es, were scanned on a Leica AT2 digital scanner. PD-L1 IHC 22C3 pharmDx assay was used to qualitatively determine percentage of PD-L1 expression on cancer cells.
At2 digital scanner
Manufactured by Leica
Sourced in Germany
The AT2 digital scanner is a high-resolution scanner designed for digitizing large-format documents and technical drawings. It features a robust construction and advanced optics to capture images with exceptional detail and accuracy. The scanner supports a wide range of media, including papers, films, and engineering blueprints, and can handle materials up to 42 inches wide. Its intuitive interface and customizable settings make it a versatile tool for various applications.
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Lab products found in correlation
3 protocols using at2 digital scanner
1
Comprehensive Tumor Immunoprofiling
2
Immunohistochemical Analysis of IPF Lung
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Serial sections of paraffin-embedded parenchymal lung tissue from IPF patients were immunolabelled for p21 using a rabbit polyclonal antibody (Cell Signaling Technology, Danvers, MA, USA, #2947, diluted 1 in 50) and detected using the Dako EnVision anti-rabbit kit (Dako Corp., Carpinteria, CA, USA) and 3,3′-diaminobenzidine (DAB, Sigma-Aldrich, St Louis, MO, USA); or stained with Masson’s Trichrome to identify collagen. Masson’s trichrome staining was performed at The Hunter Medical Research Institute’s (HMRI’s) Pathology Service laboratory and 40× magnification images digitalised using the Aperio AT2 digital scanner (Leica, Wetzlar, Hesse, Germany).
3
Histological Evaluation of Distal Colon
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Distal Colons were fixed in 10% formalin and stained with routine H&E. Histology was scored in a blinded fashion as described previously.29 (link) Briefly, histology scores include a combination of epithelium morphology change (score 0–4; same below), inflammatory cell infiltration/inflammation and mucosal ulceration. Score 1–4 indicated that the damage was observed in <25%; 25-50%; 50-75% and 75% area, respectively, while score 0 indicated normal condition. A total number of four H&E sections per animal were assessed for each sample and the average scores were used. Finally, the sum scores were determined by the combined numbers of all three parameters, representing the overall histopathology status of each sample. Representative images were acquired with a Leica AT2 digital scanner. Histology images with a score close to group average were displayed as examples.
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