Live dead viability assay kit

Zinc acetate, 2-methylimidazole (2-MI) and hemin were purchased from Aladdin Biochemical Technology Co. Ltd. (Shanghai, China). Gox and 2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) were obtained from Sigma-Aldrich (Shanghai, China). 4′,6-diamidino-2-phenylindole (DAPI) and GSH detection kit were purchased from Solarbio (Beijing, China). Cell membrane fluorescence probe (Dio), an ATP detection kit, BCA protein assay kit and live, dead viability assay kit and Protease inhibitor cocktail (100 × ) were purchased from Beyotime Biotechnology (Shanghai, China). Annexin V apoptosis kit was obtained from Yeason (Shanghai, China). Antibodies against CD45, CD3, CD8, CD4, CD11c, CD80, CD86, IFN-γ for flow cytometry were obtained from BioLegend (San Diego, California, USA). ELISA kits for analysis of cytokines were purchased from Thermo Fisher (Waltham, USA). Anti-mouse-PD-L1 antibody (BE0101) were purchased from Bio X Cell (Lebanon, USA). All other chemical reagents were analytically pure and used directly as received.