Expression levels and phosphorylation of Aurora A and histone H3 were evaluated by performing immunoblotting analysis as described previously (14 (link)). Antibodies against Aurora A (p-Aurora A) and PARP were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti-histone H3 antibody and anti-phosphorylated histone H3 (anti-p-H3; Ser10) antibody were obtained from Abcam (Cambridge, MA, USA) and Millipore (Billerica, MA, USA), respectively. The monoclonal antibody against β-actin (Sigma-Aldrich, St. Louis, MO, USA) was used for a loading control.
P aurora a
Manufactured by Cell Signaling Technology
Sourced in United States
P∼Aurora-A is a laboratory reagent that detects the phosphorylated form of the Aurora-A protein kinase. Aurora-A is a key regulator of mitotic cell division. The P∼Aurora-A product allows for the specific detection of the active, phosphorylated state of Aurora-A in cellular samples.
Automatically generated - may contain errors
3 protocols using p aurora a
1
Evaluating Aurora A and Histone H3
2
Immunoblot, Immunofluorescence, and FACS Assays
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Immunoblot, Immunofluorescence and FACS assays were performed as previously described [26 (link)]. Antibodies employed to perform these studies were the followings: p53 (D07 DAKO), p21 (Oncogene), Centrin 20h5 (Mayo Clinic, Rochester, Minnesota, USA), H2AX, Aurora-A, p∼Aurora-A, SMAD5, PLK1, cleaved-PARP, CD44 (Cell Signaling, Danvers, Massachusetts, USA), CD44, CD24 (BD Pharmigen, San Jose, California, USA), PROCR and Beta-actin (Sigma, St. Louis, Missouri, USA). Results are derived from three independent experiments with comparable outcomes.
3
NanoPro 1000 Phosphoprotein Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Lysates were analyzed using a NanoPro 1000 system (ProteinSimple Inc.) with an optimized protocol. Primary antibodies were used at 1:50 dilution for 2 hours. Antibodies employed to perform the NIA assay were the followings: p∼Aurora-A (Cell Signaling, Danvers, Massachusetts, USA) and p∼SMAD5 (Abcam, Cambridge, Massachusetts, USA). Results are derived from three independent experiments with comparable outcomes.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!