Nf kb egfp

Manufactured by Addgene

The NF-kB-eGFP is a reporter system that allows for the visualization of NF-kB activation in live cells. It consists of a plasmid expressing the enhanced green fluorescent protein (eGFP) under the control of an NF-kB responsive promoter. When NF-kB is activated, it induces the expression of eGFP, which can be detected using fluorescence microscopy or flow cytometry.

Automatically generated - may contain errors

Lab products found in correlation

7gli gfp, by Addgene (2 mentions) Torcar t a, by Addgene (2 mentions) P38ktrmcerulean3, by Addgene (2 mentions) Jnkktrmruby2, by Addgene (2 mentions) 12xcsl d1egfp, by Addgene (2 mentions) Top gfp, by Addgene (1 mentions)

2 protocols using nf kb egfp

Multifaceted Signaling Pathway Profiling in Liver Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols

Wnt/β-catenin, Notch, Hedgehog, mTOR, NF-kB, P38, JNK, ERK and PKA reporter plasmids were overexpressed liver cancer cells, and treated with lactic acid or DM-αKG^{59 (link)}. The activity levels of each signaling pathway were detected by FACS. For example, Wnt activity level = (TOP-GFP intensity)/(FOP-GFP intensity). The plasmids used in this assay are: TOP-GFP (addgene no. 35489), FOP-GFP (addgene no. 35490), 12XCSL-d1EGFP (addgene no. 47684), 7Gli:GFP (addgene no.110494), TORCAR (addgene no. 64927), NF-kB-eGFP (addgene no. 118093), TORCAR(T/A) (addgene no. 64928), p38KTRmCerulean3 (addgene no. 59155), JNKKTRmRuby2 (addgene no. 59154), PKAKTRClover (addgene no.59153), ERKKTRClover (addgene no. 59150).

Chen Z., He Q., Lu T., Wu J., Shi G., He L., Zong H., Liu B, & Zhu P. (2023). mcPGK1-dependent mitochondrial import of PGK1 promotes metabolic reprogramming and self-renewal of liver TICs. Nature Communications, 14, 1121.

+ Open protocol

+ Expand

Signaling Pathway Reporter Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Signaling pathway (including Wnt/β-catenin, Notch, Hedgehog, mTOR, NF-kB, P38, JNK, ERK and PKA) reporter plasmids and control plasmids were overexpressed in HLM006474 treated and control cells, and the activity of signaling pathway can be detected by FACS. For example, Wnt activity= (TOP-GFP intensity)/(FOP-GFP intensity).
The plasmids used in this assay are: TOP-GFP (addgene no. 35489), FOP-GFP (addgene no. 35490), 12XCSL-d1EGFP (addgene no. 47684), 7Gli:GFP (addgene no.110494), TORCAR (addgene no. 64927), TORCAR(T/A) (addgene no. 64928), NF-kB-eGFP (addgene no. 118093), p38KTRmCerulean3 (addgene no. 59155), JNKKTRmRuby2 (addgene no. 59154), ERKKTRClover (addgene no. 59150), PKAKTRClover (addgene no.59153).

Chen Z., Liu B., Huang L., Zhong X., Yan Z., & Zhu P. (2021). rtcisE2F drives liver TIC self-renewal and metastasis via m⁶A-modulated mRNA stability of E2F6 and E2F3.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!