Megm complete media

Manufactured by Lonza

Sourced in Switzerland

MEGM complete media is a cell culture medium designed to support the growth and maintenance of human mammary epithelial cells. It provides the necessary nutrients and growth factors required for these cell types.

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Lab products found in correlation

Recombinant bmp7, by R&D Systems (1 mentions) A83 01, by Bio-Techne (1 mentions) Penicillin, by Thermo Fisher Scientific (1 mentions) Hyclone fetal bovine serum fbs, by GE Healthcare (1 mentions) Hmec cells, by Lonza (1 mentions) Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Hek293t, by American Type Culture Collection (1 mentions)

Close spelling variants of this product

Complete MEGM MEGM media

3 protocols using megm complete media

3D Breast Tumor Biopsy Culturing

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The collection of fresh breast tumor biopsy material received local ethical approval from the Lothian Tissue Governance Committee (10/S1402/33). Informed consent from patients for the collection and use of tissue samples was obtained prior to use. Breast tumor biopsy material was trimmed and cut into 1mm³ pieces. Collagen type I (Wako Chemicals) was mixed with 1:1000 filtered acetic acid, DMEM/F12 (X 10) and 0.22M NaOH in a ratio of 3:5:1:1 (final collagen concentration of 0.3 mg/ml). Compounds in collagen were added to 24-well plate (0.5 ml/well) and 1 piece of tissue added. After incubation at 37°C for 1 hour to solidify the collagen, 500 μl of MEGM complete media (Lonza, Switzerland) containing compound was added per well. Treatments continued for up to 15 days. At termination of experiments, cultures were fixed in 10% phosphate buffered formalin, wax embedded, and processed for immunohistochemistry. 3D spheroid invasion assays were performed in the same manner except that DMEM culture media (prepared as above) was used, and experiments terminated by 48 hours. Results were quantified using Image J Software.

Ward C., Meehan J., Mullen P., Supuran C., Dixon J.M., Thomas J.S., Winum J.Y., Lambin P., Dubois L., Pavathaneni N.K., Jarman E.J., Renshaw L., Um I., Kay C., Harrison D.J., Kunkler I.H, & Langdon S.P. (2015). Evaluation of carbonic anhydrase IX as a therapeutic target for inhibition of breast cancer invasion and metastasis using a series of in vitro breast cancer models. Oncotarget, 6(28), 24856-24870.

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Immortalized Mammary Epithelial Cell Culture

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Immortalized mammary epithelial cells (IMECs) and IMEC-sh-p53 cells were derived as previously described (14 (link), 15 (link)). IMECs were cultured in MEGM complete media (Lonza CC-3051) with 50 μg/ml puromycin, Bovine Pituitary Extract (BPE), and 100 units/ml Penicillin/Streptomycin. Treatments with TGFβ1 and A83-01 were performed without BPE and with the addition of 0.1%BSA. H1299 cells were cultured according to ATCC guidelines. Cells were serum starved for 3 hours prior to all TGFβ treatments. Recombinant TGFβ1 (Miltenyi) was used at 500pM. A83-01 (Tocris) was used at 2uM for 1 hr prior to TGFβ1 treatment, or as indicated. LDN193189 (Stemgent Technologies) was used at 1uM, unless otherwise indicated. Recombinant BMP7 (R&D systems) was used at 50 ng/ml.

Balboni A.L., Cherukuri P., Ung M., DeCastro A.J., Cheng C, & DiRenzo J. (2015). p53 and ΔNp63α Co-regulate the Transcriptional and Cellular Response to TGFβ and BMP Signals. Molecular cancer research : MCR, 13(4), 732-742.

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Cell Culture Maintenance Protocol

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HEK
293T, HFF and A549 cell lines were
obtained from American Tissue Culture Collection (ATCC, Manassas,
VA). MDA-MB-231, MDA-MB-468, and MCF-7 cell lines were obtained from
Developmental Therapeutics Program at the National Cancer Institute
(NCI). The cells were maintained in DMEM (Life Technologies, Grand
Island, NY) with nonessential amino acids (Life Technologies) and
10% (v/v) HyClone fetal bovine serum (FBS, GE Healthcare Life Science,
Logan, UT) at 37 °C with 5% CO₂. HMEC cells were obtained
from Lonza (Walkersville, MA) and were cultured in MEGM complete media
(Lonza) supplemented with 10 μg/mL penicillin and 10 μg/mL
streptomycin (Life Technologies) at 37 °C under 5% CO₂.

Xie F., Li B.X., Kassenbrock A., Xue C., Wang X., Qian D.Z., Sears R.C, & Xiao X. (2015). Identification of a Potent Inhibitor of CREB-Mediated Gene Transcription with Efficacious in Vivo Anticancer Activity. Journal of Medicinal Chemistry, 58(12), 5075-5087.

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