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Lb agar media

Manufactured by HiMedia

LB Agar media is a common microbiological growth medium used for the cultivation of a variety of bacteria. It provides the necessary nutrients and solidifying agent for bacterial cultures to grow. The media is a formulation of Lysogeny Broth (LB) and agar, which allows for the formation of bacterial colonies on a solid surface.

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3 protocols using lb agar media

1

Synthesis and Evaluation of Polymeric Materials

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Acrylic acid, toluene, hydroquinone, tetrahydrofuran, and xylene were purchased from SD Fine Chemicals. p-toluene sulfonic acid and maleic anhydride were purchased from SRL. Polyethylene glycol (Mn=2000) was purchased from TCI chemicals; methyl iodide was purchased from Sigma Aldrich. Ethylene diamine, p-phenylene diamine, glutaraldehyde, and 1-docosanol were purchased from Merck and used as received. LB Agar media and Luria Bertani (LB) Broth purchased from Himedia, E. coli DH5α, B. Subtilis, B. Amyloliquefaciens, and C. Freundii strains were obtained from Microbial type culture collection at Chandigarh (MTCC).
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2

Chitosan-Silver Nanoparticle Synthesis

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Chitosan (NL-CSN) from
shrimp shells having a high molecular weight (MW) (∼235 kDa)
and 85% degree of deacetylation was used. Silver nitrate (AgNO3) and glacial acetic acid were from SD Fine Chem; Luria–Bertani
(LB) broth and LB agar media were from HiMedia Chemicals. Analytical-grade
chemicals were used without any further purification.
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3

Isolation and Preservation of Siderophore-Producing Bacteria

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In the present study, 23 siderophore-producing bacterial strains were taken from Culture Collection of Rhizosphere Microbiology Laboratory, Department of Environmental Microbiology, BBA University, Lucknow (Uttar Pradesh, India). Bacterial strains were grown on Luria–Bertani (LB) agar media (Himedia, Mumbai) at 28 °C for 48 h. All strains were preserved in LB slants at 4 °C and in 25% glycerol stock solution at − 80 °C.
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