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Mito gcamp5g

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Mito-GCaMP5G is a fluorescent calcium indicator designed for monitoring mitochondrial calcium dynamics. It is a genetically encoded calcium sensor that can be targeted to the mitochondria. The core function of Mito-GCaMP5G is to report changes in mitochondrial calcium levels through changes in fluorescence intensity.

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Lab products found in correlation

Pcdna3.1 vector, by Thermo Fisher Scientific (2 mentions) Gfp rab7, by Addgene (2 mentions) Lipofectamine ltx transfection reagent, by Thermo Fisher Scientific (2 mentions) Mkeima red mito 7, by Addgene (2 mentions) Percivalhr, by Addgene (2 mentions) Egfp lc3, by Addgene (2 mentions)

Spelling variants of this product

GCaMP5G (5 citations)

2 protocols using mito gcamp5g

1

Fluorescent Markers for Neuronal Organelles

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
GFP-SNPH and GFP SNPH1–469 plasmids were gifts from Sheng laboratory at NIH. For non-GFP SNPH vectors, we cloned SNPH and SNPH1–469 into pcDNA3.1 vectors (Invitrogen) at HindIII and Xho1 restriction sites. We used Addgene plasmids Mito-GCaMP5G (Addgene plasmid #105009; Kwon et al., 2016 (link)), PercivalHR (Addgene plasmid #49082; Tantama et al., 2013 (link)), mKeima-Red-Mito-7 (Addgene plasmid #56018, a gift from Michael Davidson), EGFP-LC3 (Addgene plasmid #11546; Jackson et al., 2005 (link)) and GFP-RAB7 (Addgene plasmid #12605; Choudhury et al., 2002 (link)). pF(UG) hSyn Lamp1-msGFP (#JV012) plasmid was a gift from Jason Vevea/Edwin R. Chapman at UW, Madison. These plasmids were transfected in primary hippocampal neurons on DIV4 using lipofectamine LTX transfection reagent (Thermo Fisher scientific) for 5 hr as described in manufacturer’s protocol.
Joshi D.C., Zhang C.L., Babujee L., Vevea J.D., August B.K., Sheng Z.H., Chapman E.R., Gomez T.M, & Chiu S.Y. (2019). Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell reports, 29(3), 685-696.e5.
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2

Fluorescent Markers for Neuronal Organelles

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
GFP-SNPH and GFP SNPH1–469 plasmids were gifts from Sheng laboratory at NIH. For non-GFP SNPH vectors, we cloned SNPH and SNPH1–469 into pcDNA3.1 vectors (Invitrogen) at HindIII and Xho1 restriction sites. We used Addgene plasmids Mito-GCaMP5G (Addgene plasmid #105009; Kwon et al., 2016 (link)), PercivalHR (Addgene plasmid #49082; Tantama et al., 2013 (link)), mKeima-Red-Mito-7 (Addgene plasmid #56018, a gift from Michael Davidson), EGFP-LC3 (Addgene plasmid #11546; Jackson et al., 2005 (link)) and GFP-RAB7 (Addgene plasmid #12605; Choudhury et al., 2002 (link)). pF(UG) hSyn Lamp1-msGFP (#JV012) plasmid was a gift from Jason Vevea/Edwin R. Chapman at UW, Madison. These plasmids were transfected in primary hippocampal neurons on DIV4 using lipofectamine LTX transfection reagent (Thermo Fisher scientific) for 5 hr as described in manufacturer’s protocol.
Joshi D.C., Zhang C.L., Babujee L., Vevea J.D., August B.K., Sheng Z.H., Chapman E.R., Gomez T.M, & Chiu S.Y. (2019). Inappropriate Intrusion of an Axonal Mitochondrial Anchor into Dendrites Causes Neurodegeneration. Cell reports, 29(3), 685-696.e5.
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