Cell culture plastics were purchased from Becton Dickinson (Lincoln Park, NJ, USA). Fetal bovine serum (FBS), phosphate-buffered saline (PBS), L-glutamine, trypsin, and antibiotics were purchased from Gibco (Life Technologies, Carlsbad, CA, USA). Trans-polydatin with a purity grade higher than 99% was provided by Glures S.r.l., Academic Spin-Off of Ca’ Foscari University of Venice (Venice, Italy). Minimum Essential Medium Eagle Alpha Modification (α-MEM) was purchased from Gibco, Thermo Fisher Scientific, Inc. (Waltham, MA, USA). All other regents used in this study were of analytical grade. Polycaprolactone was purchased from Sigma-Aldrich (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Chloroform and ethanol were purchased from VWR International (VWR International, Radnor, PA, USA).
Cell culture plastics
Manufactured by BD
Sourced in United States
Cell culture plastics are laboratory equipment designed for the growth and maintenance of cells in controlled environments. These products provide a surface for cells to adhere to and proliferate, facilitating various research and experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Galactose, by Merck Group (3 mentions)
Anti v5 hrp antibody, by Thermo Fisher Scientific (3 mentions)
Raffinose, by Merck Group (3 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Yeast dob, by MP Biomedicals (2 mentions)
Mutagenesis reagents, by Agilent Technologies (2 mentions)
Glucose, by Merck Group (2 mentions)
Chloroform, by Avantor (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
Minimum essential medium eagle alpha modification α mem, by Thermo Fisher Scientific (1 mentions)
Dextrose, by Merck Group (1 mentions)
Trypsin solution, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Glutamine, by Merck Group (1 mentions)
Bicinchoninic acid (bca), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium, by Merck Group (1 mentions)
Bradford protein assay, by Thermo Fisher Scientific (1 mentions)
Oxyblottm protein oxidation detection kit, by Merck Group (1 mentions)
Chemicals for sds page, by Bio-Rad (1 mentions)
Penicillin, by Merck Group (1 mentions)
6 protocols using cell culture plastics
1
Polycaprolactone Scaffold Fabrication
2
Transpolydatin Cell Culture Protocol
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Cell culture plastics were purchased from Becton Dickinson (Lincoln Park, NJ). Fetal bovine serum (FBS), phosphate-buffered saline (PBS), L-glutamine, trypsin, and antibiotics were purchased from Gibco (Life Technologies, Carlsbad, CA). Transpolydatin with a purity grade higher than 99% has been provided by Glures S.r.l., Academic Spin-Off of Ca' Foscari University of Venice (Venice, Italy).
3
Yeast Cell Culture and Protein Purification
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Yeast DOB were obtained in powder form from MP Biomedicals (Solon, OH, USA). Cell culture plastics were from BD Falcon (San Jose, CA, USA). Dextrose, galactose, and raffinose were obtained from Sigma (St. Louis MO, USA). Glass beads for yeast cell lysis were from B. Braun Biotech (Allentown, PA, USA). Anti-V5-HRP antibody was from Invitrogen (Carlsbad, CA, USA). Mutagenesis reagents were obtained from Agilent (Santa Clara, CA, USA). All other chemicals were reagent grade or better, were purchased from Sigma (St. Louis MO, USA) and used without additional purification.
4
Yeast Cell Lysis and Protein Purification
5
Yeast Cell Lysis and Protein Expression
6
Quantitative Cell Culture Assay Protocol
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Dulbecco's Modified Eagle Medium (4.5 mg mL -1 glucose), penicillin, streptomycin, glutamine, trypsin solution for cell culture (0.05% (w/v)) were purchased from Sigma-Aldrich (Saint-Quentin Fallavier, France) and fetal bovine serum from Invitrogen Life technologies (Cergy Pontoise, France). Cell culture plastics were purchased from BD Falcon (Le Pont de Claix, France). Cell proliferation kit II (XTT) was obtained from Roche Diagnostics (Meylan, France). Western blot reagents and membranes were purchased from GE Healthcare (Saclay, France). The bicinchoninic acid (BCA) and Bradford protein assays were purchased from Thermo scientific (Villebon-sur-Yvette, France) and from Expedeon (San Diego, USA), respectively and chemicals for SDS/PAGE from Bio-Rad (Marnes La Coquette, France).
OxyBlot TM protein oxidation detection kit was purchased from Millipore (Molsheim, France).
All other reagents were obtained from Sigma-Aldrich, unless specified.
OxyBlot TM protein oxidation detection kit was purchased from Millipore (Molsheim, France).
All other reagents were obtained from Sigma-Aldrich, unless specified.
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